Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | cobalt-carbon bond cleavage depends obligatorily on substrate binding in all adenosylcobalamin-dependent enzymes, with the exception of ribonucleotide triphosphate reductase, which can cleave the bond upon binding a nucleotide triphosphate activator | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
500000 | - |
calculated holoenzyme molecular mass | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanolamine | Salmonella enterica subsp. enterica serovar Typhimurium | The family of vitamin B12 coenzyme-dependent enzymes catalyzes the radical-mediated cleavage of unactivated C-H bonds and associated 1,2-cross-migrations of hydrogen and alkyl, carbonyl, hydroxyl, or amino groups | acetaldehyde + NH3 | - |
ir | |
additional information | Salmonella enterica subsp. enterica serovar Typhimurium | the radical intermediate is a substrate radical | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of the purified enzyme with aminoethanol as substrate is 20-30 micromol/min/mg. | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanolamine | The family of vitamin B12 coenzyme-dependent enzymes catalyzes the radical-mediated cleavage of unactivated C-H bonds and associated 1,2-cross-migrations of hydrogen and alkyl, carbonyl, hydroxyl, or amino groups | Salmonella enterica subsp. enterica serovar Typhimurium | acetaldehyde + NH3 | - |
ir | |
additional information | the radical intermediate is a substrate radical | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ethanolamine ammonia-lyase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
ethanolamine deaminase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
ethanolamine | at 0°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
55 | - |
ethanolamine | at 22 °C | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.45 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adenosylcobalamin | vitamine B12 coenzyme | Salmonella enterica subsp. enterica serovar Typhimurium |