Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.3.1.7 extracted from

  • Akita, K.; Hieda, N.; Baba, N.; Kawaguchi, S.; Sakamoto, H.; Nakanishi, Y.; Yamanishi, M.; Mori, K.; Toraya, T.
    Purification and some properties of wild-type and N-terminal-truncated ethanolamine ammonia-lyase of Escherichia coli (2010), J. Biochem., 147, 83-93.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information N-terminal truncated enzyme, similar to wild-type enzyme in catalytic properties, more resistant to p-chloromercuribenzoate than wild-type enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
Escherichia coli
p-chlormercuribenzoate
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
adenosylcobalamin truncated enzymes, value between 0.000041 and 0.000078 Escherichia coli
additional information
-
ethanolamine truncated enzymes, value between 0.0059 and 0.0076 Escherichia coli
0.000055
-
adenosylcobalamin wild-type enzyme Escherichia coli
0.0082
-
ethanolamine wild-type enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
31% yield after DEAE-cellulose column Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
truncated enzymes, after purificaton, value between 12.45 and 63.1 Escherichia coli
55.1
-
wild-type enzyme, after purification Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanolamine assay at pH 8.0, 37°C, reaction terminated by adding potassium citrate buffer Escherichia coli acetaldehyde + NH3
-
?

Subunits

Subunits Comment Organism
heterodecamer alphabeta, 6*49000, 6*32000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
ethanolamine ammonia-lyase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
adenosylcobalamin
-
Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
truncated enzymes, value around 0.0013 Escherichia coli p-chlormercuribenzoate
additional information
-
truncated enzymes, value between 0.07 to 0.24 Escherichia coli iodoacetamide
0.0003
-
wild-type enzyme Escherichia coli p-chlormercuribenzoate
0.07
-
wild-type enzyme Escherichia coli iodoacetamide