Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | N-terminal truncated enzyme, similar to wild-type enzyme in catalytic properties, more resistant to p-chloromercuribenzoate than wild-type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Escherichia coli | |
p-chlormercuribenzoate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
adenosylcobalamin | truncated enzymes, value between 0.000041 and 0.000078 | Escherichia coli | |
additional information | - |
ethanolamine | truncated enzymes, value between 0.0059 and 0.0076 | Escherichia coli | |
0.000055 | - |
adenosylcobalamin | wild-type enzyme | Escherichia coli | |
0.0082 | - |
ethanolamine | wild-type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
31% yield after DEAE-cellulose column | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
truncated enzymes, after purificaton, value between 12.45 and 63.1 | Escherichia coli |
55.1 | - |
wild-type enzyme, after purification | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanolamine | assay at pH 8.0, 37°C, reaction terminated by adding potassium citrate buffer | Escherichia coli | acetaldehyde + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodecamer | alphabeta, 6*49000, 6*32000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ethanolamine ammonia-lyase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adenosylcobalamin | - |
Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
truncated enzymes, value around 0.0013 | Escherichia coli | p-chlormercuribenzoate | |
additional information | - |
truncated enzymes, value between 0.07 to 0.24 | Escherichia coli | iodoacetamide | |
0.0003 | - |
wild-type enzyme | Escherichia coli | p-chlormercuribenzoate | |
0.07 | - |
wild-type enzyme | Escherichia coli | iodoacetamide |