Any feedback?
No. of entries
Information on EC 3.2.2.3 - uridine nucleosidase
for references in articles please use BRENDA:EC3.2.2.3
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 3 Hydrolases
3.2 Glycosylases
3.2.2 Hydrolysing N-glycosyl compounds
3.2.2.3 uridine nucleosidase
The enzyme appears in viruses and cellular organisms
- 3.2.2.3
-
salvage
- uracil
- purine
-
protozoan
- deaminase
-
crithidia
- fasciculata
-
baker
- orotate
- ribonucleosides
-
schramm
- vaginalis
-
trichomonas
- glycosylase
- pharmacology
showSynonyms
uridine hydrolase, uridine nucleosidase, inosine-uridine nucleoside hydrolase, uridine ribohydrolase, nucleoside hydrolase 1, urh1p, uridine-cytidine n-ribohydrolase, uridine nucleoside ribohydrolase, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
NSH1
nucleosidase, uridine
-
-
-
-
URH1
Urh1p
uridine hydrolase
Uridine ribohydrolase
-
-
-
-
uridine-cytidine N-ribohydrolase
uridine-preferring pyrimidine nucleoside hydrolase
uridine-ribohydrolase 1
uridine hydrolase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
uridine + H2O = D-ribose + uracil
-
-
-
-
uridine + H2O = D-ribose + uracil
substrate binding site, active site structure, His254 is important, catalytic mechanism
uridine + H2O = D-ribose + uracil
catalytic mechanism with catalytic triad consisting of Tyr223, Tyr227, and His239, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of N-glycosyl bond
hydrolysis of N-glycosyl bond
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
MetaCyc
NAD salvage (plants), pyrimidine ribonucleosides salvage II, pyrimidine ribonucleosides salvage III
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
uridine ribohydrolase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
9025-47-2
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
5-fluorocytidine + H2O
D-ribose + 5-fluorocytosine
Substrates: -
Products: -
Products: -
?
5-fluorouridine + H2O
D-ribose + 5-fluorouracil
Substrates: -
Products: -
Products: -
?
5-methyluridine + H2O
5-methyluracil + D-ribose
-
Substrates: -
Products: -
Products: -
?
5-methyluridine + H2O
D-ribose + 5-methyluracil
-
Substrates: -
Products: -
Products: -
?
deoxycytidine + H2O
D-deoxyribose + cytosine
Substrates: -
Products: -
Products: -
?
isopentenyladenine riboside + H2O
D-ribose + N6-isopentenyladenine
-
Substrates: -
Products: -
Products: -
?
adenosine + H2O
D-ribose + adenine
Substrates: 15% of the activity with uridine
Products: -
Products: -
?
adenosine + H2O
D-ribose + adenine
Substrates: 3.5% of the activity with uridine
Products: -
Products: -
?
cytidine + H2O
D-ribose + cytosine
-
Substrates: 3.9% of the activity with uridine
Products: -
Products: -
?
cytidine + H2O
D-ribose + cytosine
Substrates: -
Products: -
Products: -
?
cytidine + H2O
D-ribose + cytosine
Substrates: 5.0% of the activity with uridine
Products: -
Products: -
?
inosine + H2O
D-ribose + hypoxanthine
-
Substrates: 22% of the activity with uridine
Products: -
Products: -
?
inosine + H2O
D-ribose + hypoxanthine
Substrates: 9.3% of the activity with uridine
Products: -
Products: -
?
inosine + H2O
D-ribose + hypoxanthine
Substrates: 9.5% of the activity with uridine
Products: -
Products: -
?
nicotinamide riboside + H2O
nicotinamide + D-ribose
-
Substrates: the uridine hydrolase is 100fold more active as a nicotinamide riboside hydrolase than as a uridine hydrolase
Products: -
Products: -
?
nicotinamide riboside + H2O
nicotinamide + D-ribose
-
Substrates: the uridine hydrolase is 100fold more active as a nicotinamide riboside hydrolase than as a uridine hydrolase
Products: -
Products: -
?
nicotinic acid riboside + H2O
nicotinic acid + D-ribose
-
Substrates: -
Products: -
Products: -
?
nicotinic acid riboside + H2O
nicotinic acid + D-ribose
-
Substrates: -
Products: -
Products: -
?
nicotinic acid riboside methyl ester + H2O
?
-
Substrates: -
Products: -
Products: -
?
nicotinic acid riboside methyl ester + H2O
?
-
Substrates: -
Products: -
Products: -
?
uridine + H2O
D-ribose + uracil
Substrates: -
Products: -
Products: -
?
uridine + H2O
D-ribose + uracil
-
Substrates: isoform NSH1 shows highest hydrolysis capacity for uridine
Products: -
Products: -
?
uridine + H2O
D-ribose + uracil
-
Substrates: best substrate
Products: -
Products: -
?
uridine + H2O
D-ribose + uracil
Substrates: enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
Products: -
Products: -
?
uridine + H2O
uracil + D-ribose
-
Substrates: -
Products: -
Products: -
?
uridine + H2O
uracil + D-ribose
-
Substrates: -
Products: -
Products: -
?
xanthosine + H2O
D-ribose + xanthine
-
Substrates: 61% of the activity with uridine
Products: -
Products: -
?
xanthosine + H2O
D-ribose + xanthine
Substrates: 34% of the activity with uridine
Products: -
Products: -
?
xanthosine + H2O
D-ribose + xanthine
Substrates: 53% of the activity with uridine
Products: -
Products: -
?
additional information
?
-
Substrates: the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity, overview. No activity with 2'-OH and 3'-OH deoxynucleosides. Nucleosides in which additional groups are added to the exocyclic N6 amino group also exhibit no activity. Nucleosides lacking the 5'-OH group or with the 2'-OH group in the arabino configuration exhibit greatly reduced activity. Purine nucleosides and pyrimidine nucleosides in which the N7 or N3 nitrogens respectively are replaced with carbon also have no activity
Products: -
Products: -
?
additional information
?
-
-
Substrates: adenosine is a poor substrates, no activity with guanosine, trans-zeatin riboside, and cytokinin riboside, substrate specificity, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in the metabolism of pyridine nucleotides
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in cytidine metabolism
Products: -
Products: -
?
additional information
?
-
-
Substrates: key enzyme of the pyrimidine salvage pathway, overview, enzyme is required for growth of mutants deficient in uridine kinase activity
Products: -
Products: -
?
additional information
?
-
Substrates: physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: inosine, adenosine, guanosine, and thymidine are poor substrates
Products: -
Products: -
?
additional information
?
-
Substrates: substrate specificity, enzyme possesses no phosphorylase activity
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity, enzyme possesses no phosphorylase activity
Products: -
Products: -
?
additional information
?
-
-
Substrates: no or almost no activity with guanosine, trans-zeatin riboside, and cytokinin riboside, substrate specificity, overview
Products: -
Products: -
?
additional information
?
-
Substrates: no or almost no activity with guanosine, trans-zeatin riboside, and cytokinin riboside, substrate specificity, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: poor activity with cytidine, guanosine, trans-zeatin riboside, and cytokinin riboside, substrate specificity, overview
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
uridine + H2O
D-ribose + uracil
Substrates: enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in the metabolism of pyridine nucleotides
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in cytidine metabolism
Products: -
Products: -
?
additional information
?
-
-
Substrates: key enzyme of the pyrimidine salvage pathway, overview, enzyme is required for growth of mutants deficient in uridine kinase activity
Products: -
Products: -
?
additional information
?
-
Substrates: physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
Urh1 activity is unaffected by the addition of MgCl2
additional information
-
the enzyme does not require metal ions for activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Tris
-
twice recrystallized from aqueous acetone, 30 mM, 50% inhibition
additional information
-
molecular mechanism for the in vitro antitrichomonal activity of these proton-pump inhibitors, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.045
nicotinic acid riboside methyl ester
-
in 50 mM Tris-HCl, pH 7.0
2.1
uridine
recombinant enzyme expressed in Saccharomyces cerevisiae
additional information
additional information
-
substrate specificity and kinetics, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2.3
nicotinic acid riboside methyl ester
-
in 50 mM Tris-HCl, pH 7.0
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3
5-fluorocytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
0.6
5-fluorouridine
pH 7.5, 37°C, wild-type enzyme, versus uridine
3
deoxycytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
2
ribosylthymine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY