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Information on EC 3.2.2.3 - uridine nucleosidase
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EC Tree
3.2.2.3 uridine nucleosidaseSpecify your search results
Word Map
- 3.2.2.3
-
salvage
- uracil
- purine
-
protozoan
- deaminase
-
crithidia
- fasciculata
-
baker
- orotate
- ribonucleosides
-
schramm
- vaginalis
-
trichomonas
- glycosylase
- pharmacology
The enzyme appears in viruses and cellular organisms
Synonyms
uridine hydrolase, uridine nucleosidase, inosine-uridine nucleoside hydrolase, uridine ribohydrolase, nucleoside hydrolase 1, urh1p, uridine-cytidine n-ribohydrolase, uridine nucleoside ribohydrolase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
NSH1
nucleosidase, uridine
-
-
-
-
URH1
Urh1p
uridine hydrolase
Uridine ribohydrolase
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-
-
-
uridine-cytidine N-ribohydrolase
uridine-preferring pyrimidine nucleoside hydrolase
uridine-ribohydrolase 1
uridine hydrolase
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-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
uridine + H2O = D-ribose + uracil
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-
-
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uridine + H2O = D-ribose + uracil
substrate binding site, active site structure, His254 is important, catalytic mechanism
uridine + H2O = D-ribose + uracil
catalytic mechanism with catalytic triad consisting of Tyr223, Tyr227, and His239, overview
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of N-glycosyl bond
hydrolysis of N-glycosyl bond
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-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
uridine ribohydrolase
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CAS REGISTRY NUMBER
COMMENTARY
9025-47-2
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
isopentenyladenine riboside + H2O
D-ribose + N6-isopentenyladenine
-
-
-
-
?
cytidine + H2O
D-ribose + cytosine
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3.9% of the activity with uridine
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-
?
inosine + H2O
D-ribose + hypoxanthine
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22% of the activity with uridine
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-
?
inosine + H2O
D-ribose + hypoxanthine
9.3% of the activity with uridine
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-
?
inosine + H2O
D-ribose + hypoxanthine
9.5% of the activity with uridine
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-
?
nicotinamide riboside + H2O
nicotinamide + D-ribose
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the uridine hydrolase is 100fold more active as a nicotinamide riboside hydrolase than as a uridine hydrolase
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-
?
nicotinamide riboside + H2O
nicotinamide + D-ribose
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the uridine hydrolase is 100fold more active as a nicotinamide riboside hydrolase than as a uridine hydrolase
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-
?
nicotinic acid riboside + H2O
nicotinic acid + D-ribose
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-
-
-
?
uridine + H2O
D-ribose + uracil
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isoform NSH1 shows highest hydrolysis capacity for uridine
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-
?
uridine + H2O
D-ribose + uracil
enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
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-
?
uridine + H2O
uracil + D-ribose
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-
-
-
?
xanthosine + H2O
D-ribose + xanthine
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61% of the activity with uridine
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-
?
additional information
?
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the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine
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-
?
additional information
?
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substrate specificity, overview. No activity with 2'-OH and 3'-OH deoxynucleosides. Nucleosides in which additional groups are added to the exocyclic N6 amino group also exhibit no activity. Nucleosides lacking the 5'-OH group or with the 2'-OH group in the arabino configuration exhibit greatly reduced activity. Purine nucleosides and pyrimidine nucleosides in which the N7 or N3 nitrogens respectively are replaced with carbon also have no activity
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-
?
additional information
?
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adenosine is a poor substrates, no activity with guanosine, trans-zeatin riboside, and cytokinin riboside, substrate specificity, overview
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-
?
additional information
?
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the enzyme is involved in the metabolism of pyridine nucleotides
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?
additional information
?
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the enzyme is involved in cytidine metabolism
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?
additional information
?
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key enzyme of the pyrimidine salvage pathway, overview, enzyme is required for growth of mutants deficient in uridine kinase activity
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?
additional information
?
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physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
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?
additional information
?
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physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
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?
additional information
?
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inosine, adenosine, guanosine, and thymidine are poor substrates
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-
?
additional information
?
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substrate specificity, enzyme possesses no phosphorylase activity
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?
additional information
?
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substrate specificity, enzyme possesses no phosphorylase activity
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?
additional information
?
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no or almost no activity with guanosine, trans-zeatin riboside, and cytokinin riboside, substrate specificity, overview
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?
additional information
?
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no or almost no activity with guanosine, trans-zeatin riboside, and cytokinin riboside, substrate specificity, overview
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-
?
additional information
?
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poor activity with cytidine, guanosine, trans-zeatin riboside, and cytokinin riboside, substrate specificity, overview
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-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
uridine + H2O
D-ribose + uracil
enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
-
-
?
additional information
?
-
-
the enzyme is involved in the metabolism of pyridine nucleotides
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-
?
additional information
?
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the enzyme is involved in cytidine metabolism
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-
?
additional information
?
-
-
key enzyme of the pyrimidine salvage pathway, overview, enzyme is required for growth of mutants deficient in uridine kinase activity
-
-
?
additional information
?
-
physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
-
-
?
additional information
?
-
-
physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
Urh1 activity is unaffected by the addition of MgCl2
additional information
-
the enzyme does not require metal ions for activity
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Tris
-
twice recrystallized from aqueous acetone, 30 mM, 50% inhibition
additional information
-
molecular mechanism for the in vitro antitrichomonal activity of these proton-pump inhibitors, overview
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.045
nicotinic acid riboside methyl ester
-
in 50 mM Tris-HCl, pH 7.0
2.1
uridine
recombinant enzyme expressed in Saccharomyces cerevisiae
additional information
additional information
-
substrate specificity and kinetics, overview
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2.3
nicotinic acid riboside methyl ester
-
in 50 mM Tris-HCl, pH 7.0
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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