Cloned (Comment) | Organism |
---|---|
gene rihC, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS E. | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.408 | - |
uridine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli | |
0.416 | - |
adenosine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli | |
0.421 | - |
ribothymidine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli | |
0.422 | - |
Inosine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli | |
0.454 | - |
Xanthosine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli | |
0.682 | - |
cytidine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33642 | - |
2 * 36000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 33642, His-tagged enzyme, sequence calculation | Escherichia coli |
36000 | - |
2 * 36000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 33642, His-tagged enzyme, sequence calculation | Escherichia coli |
69000 | - |
recombinant His-tagged enzyme, gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene rihC | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS E. by nickel affinity chromatography, ultrafiltration, dialysis, and anion exchange chromatography | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
uridine + H2O = D-ribose + uracil | catalytic mechanism with catalytic triad consisting of Tyr223, Tyr227, and His239, overview | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine + H2O | - |
Escherichia coli | D-ribose + adenine | - |
? | |
cytidine + H2O | - |
Escherichia coli | D-ribose + cytosine | - |
? | |
inosine + H2O | - |
Escherichia coli | D-ribose + hypoxanthine | - |
? | |
additional information | substrate specificity, overview. No activity with 2'-OH and 3'-OH deoxynucleosides. Nucleosides in which additional groups are added to the exocyclic N6 amino group also exhibit no activity. Nucleosides lacking the 5'-OH group or with the 2'-OH group in the arabino configuration exhibit greatly reduced activity. Purine nucleosides and pyrimidine nucleosides in which the N7 or N3 nitrogens respectively are replaced with carbon also have no activity | Escherichia coli | ? | - |
? | |
ribothymidine + H2O | - |
Escherichia coli | D-ribose + ? | - |
? | |
uridine + H2O | best substrate | Escherichia coli | D-ribose + uracil | - |
? | |
xanthosine + H2O | - |
Escherichia coli | D-ribose + xanthine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 36000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 33642, His-tagged enzyme, sequence calculation | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
inosine-uridine nucleoside hydrolase | - |
Escherichia coli |
RihC | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.12 | - |
cytidine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli | |
1.15 | - |
adenosine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli | |
1.29 | - |
ribothymidine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli | |
4.31 | - |
Inosine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli | |
6.3 | - |
Xanthosine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli | |
10.85 | - |
uridine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH profile is bell-shaped with the enzyme showing significant activity over a wide pH range, 50% activity at pH 5.0 and pH 9.0 | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
adenosine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli | |
10.2 | - |
Inosine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli | |
13 | - |
Xanthosine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli | |
16 | - |
cytidine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli | |
26 | - |
uridine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli | |
31 | - |
ribothymidine | pH 7.2, 32°C, recombinant His-tagged enzyme | Escherichia coli |