BRENDA - Enzyme Database show
show all sequences of 3.2.2.3

The URH1 uridine ribohydrolase of Saccharomyces cerevisiae

Kurtz, J.E.; Exinger, F.; Erbs, P.; Jund, R.; Curr. Genet. 41, 132-141 (2002)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
H254A
sited-directed mutagenesis, active site mutant, 30fold decreased Vmax and unaltered Km compared to the wild-type enzyme
Saccharomyces cerevisiae
additional information
construction of urh115 disruption mutant strains
Saccharomyces cerevisiae
Inhibitors
Inhibitors
Commentary
Organism
Structure
5-fluorocytidine
noncompetitive
Saccharomyces cerevisiae
5-fluorouridine
; competitive
Saccharomyces cerevisiae
cytidine
noncompetitive
Saccharomyces cerevisiae
deoxycytidine
competitive
Saccharomyces cerevisiae
Inosine
noncompetitive
Saccharomyces cerevisiae
ribosylthymine
competitive
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Saccharomyces cerevisiae
physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
?
-
-
-
uridine + H2O
Saccharomyces cerevisiae
enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
D-ribose + uracil
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
Q04179
wild-type strain MATaa and mutant derivatives, gene URH1
-
Reaction
Reaction
Commentary
Organism
uridine + H2O = D-ribose + uracil
substrate binding site, active site structure, His254 is important, catalytic mechanism
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-fluorocytidine + H2O
-
655283
Saccharomyces cerevisiae
D-ribose + 5-fluorocytosine
-
-
-
?
5-fluorouridine + H2O
-
655283
Saccharomyces cerevisiae
D-ribose + 5-fluorouracil
-
-
-
?
cytidine + H2O
-
655283
Saccharomyces cerevisiae
D-ribose + cytosine
-
-
-
?
deoxycytidine + H2O
-
655283
Saccharomyces cerevisiae
D-deoxyribose + cytosine
-
-
-
?
additional information
physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
655283
Saccharomyces cerevisiae
?
-
-
-
-
additional information
substrate specificity, enzyme possesses no phosphorylase activity
655283
Saccharomyces cerevisiae
?
-
-
-
-
uridine + H2O
-
655283
Saccharomyces cerevisiae
D-ribose + uracil
-
-
-
?
uridine + H2O
enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
655283
Saccharomyces cerevisiae
D-ribose + uracil
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Saccharomyces cerevisiae
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.6
-
5-fluorouridine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
2
-
cytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
2
-
ribosylthymine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3
-
5-fluorocytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3
-
deoxycytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3
-
Inosine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
H254A
sited-directed mutagenesis, active site mutant, 30fold decreased Vmax and unaltered Km compared to the wild-type enzyme
Saccharomyces cerevisiae
additional information
construction of urh115 disruption mutant strains
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5-fluorocytidine
noncompetitive
Saccharomyces cerevisiae
5-fluorouridine
-
Saccharomyces cerevisiae
5-fluorouridine
competitive
Saccharomyces cerevisiae
cytidine
noncompetitive
Saccharomyces cerevisiae
deoxycytidine
competitive
Saccharomyces cerevisiae
Inosine
noncompetitive
Saccharomyces cerevisiae
ribosylthymine
competitive
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.6
-
5-fluorouridine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
2
-
cytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
2
-
ribosylthymine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3
-
5-fluorocytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3
-
deoxycytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3
-
Inosine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Saccharomyces cerevisiae
physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
?
-
-
-
uridine + H2O
Saccharomyces cerevisiae
enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
D-ribose + uracil
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-fluorocytidine + H2O
-
655283
Saccharomyces cerevisiae
D-ribose + 5-fluorocytosine
-
-
-
?
5-fluorouridine + H2O
-
655283
Saccharomyces cerevisiae
D-ribose + 5-fluorouracil
-
-
-
?
cytidine + H2O
-
655283
Saccharomyces cerevisiae
D-ribose + cytosine
-
-
-
?
deoxycytidine + H2O
-
655283
Saccharomyces cerevisiae
D-deoxyribose + cytosine
-
-
-
?
additional information
physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
655283
Saccharomyces cerevisiae
?
-
-
-
-
additional information
substrate specificity, enzyme possesses no phosphorylase activity
655283
Saccharomyces cerevisiae
?
-
-
-
-
uridine + H2O
-
655283
Saccharomyces cerevisiae
D-ribose + uracil
-
-
-
?
uridine + H2O
enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
655283
Saccharomyces cerevisiae
D-ribose + uracil
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Saccharomyces cerevisiae
Other publictions for EC 3.2.2.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751844
Baccolini
AMP and GMP catabolism in Ara ...
Arabidopsis thaliana
Plant Cell
31
734-751
2019
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
731361
Arivett
Characterization of inosine-ur ...
Escherichia coli
Biochim. Biophys. Acta
1844
656-662
2014
-
-
1
-
-
-
-
6
-
-
3
-
-
2
-
-
1
1
-
-
-
-
7
1
1
-
-
6
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
-
3
-
-
-
-
1
-
-
-
-
7
1
1
-
-
6
1
1
-
-
-
-
-
-
6
6
731446
Shea
Identification of proton-pump ...
Trichomonas vaginalis
Bioorg. Med. Chem. Lett.
24
1080-1084
2014
-
-
-
-
-
-
4
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
3
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
732656
Kopecna
Structure and function of nucl ...
Physcomitrella patens, Zea mays
Plant Physiol.
163
1568-1583
2013
-
-
2
-
1
-
-
9
-
-
-
3
-
3
-
-
-
-
-
-
-
-
19
-
2
-
-
8
2
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
9
-
-
-
3
-
-
-
-
-
-
-
-
19
-
3
-
-
8
3
-
-
-
-
3
4
-
8
8
716542
Jung
Arabidopsis nucleoside hydrola ...
Arabidopsis thaliana
Plant J.
65
703-711
2011
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
698901
Belenky
Nicotinamide riboside and nico ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4742
J. Biol. Chem.
284
158-164
2009
-
-
1
-
-
-
2
4
-
1
-
-
-
26
-
-
-
-
-
-
-
-
8
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
4
-
1
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
700741
Jung
Uridine-ribohydrolase is a key ...
Arabidopsis thaliana, Oryza sativa
Plant Cell
21
876-891
2009
-
-
2
-
1
-
-
3
-
-
-
-
-
2
-
-
-
-
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
710272
Mach
-
Uridine ribohydrolase and the ...
Arabidopsis thaliana
Plant Cell
21
0000
2009
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
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-
-
-
-
-
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-
5
-
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1
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-
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-
-
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-
-
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-
-
-
-
-
683968
Katahira
-
Dual function of pyrimidine me ...
Solanum tuberosum
Physiol. Plant.
127
38-43
2006
-
-
-
-
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1
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2
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2
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
638539
Mitterbauer
Saccharomyces cerevisiae URH1 ...
Crithidia fasciculata, Homo sapiens, Saccharomyces cerevisiae
Appl. Environ. Microbiol.
68
1336-1343
2002
-
1
3
-
1
-
-
-
-
-
-
7
-
5
-
-
-
-
-
-
-
-
8
-
1
-
-
-
-
-
-
-
-
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-
1
3
-
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
8
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
655283
Kurtz
The URH1 uridine ribohydrolase ...
Saccharomyces cerevisiae
Curr. Genet.
41
132-141
2002
-
-
-
-
2
-
6
-
-
-
-
2
-
4
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
6
-
-
-
-
-
-
-
2
-
-
7
6
-
-
-
-
2
-
-
-
-
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
326402
Kurtz
New insights into the pyrimidi ...
Saccharomyces cerevisiae
Curr. Genet.
36
130-136
1999
-
-
-
-
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-
-
-
-
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-
1
-
2
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3
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1
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3
-
-
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-
-
-
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-
-
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-
-
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-
326393
Lee
-
Uridine nucleosidase from malt ...
Hordeum vulgare
J. Am. Soc. Brew. Chem.
45
131-135
1987
-
-
-
-
-
-
2
1
-
-
2
-
-
1
-
-
1
-
-
-
1
-
1
1
1
-
2
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
2
-
-
-
-
1
-
-
1
-
1
1
1
-
2
-
1
1
1
-
-
-
-
-
-
-
326394
Magni
Uridine nucleosidase from yeas ...
Saccharomyces cerevisiae
Methods Enzymol.
51
290-296
1978
-
-
-
-
-
-
14
2
-
1
2
-
-
2
-
-
-
-
-
-
1
2
2
1
-
-
-
-
1
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-
-
-
-
-
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-
-
-
-
-
-
-
14
-
2
-
1
2
-
-
-
-
-
-
-
1
2
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
326395
Magni
Baker's yeast uridine nucleosi ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
69
724-730
1976
-
-
-
-
-
-
9
-
-
1
-
-
-
2
-
-
-
-
-
-
-
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1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
326398
Magni
Bakers yeast uridine nucleosid ...
Saccharomyces cerevisiae
J. Biol. Chem.
250
9-13
1975
-
-
-
-
-
-
4
2
-
-
2
-
-
2
-
-
-
-
-
-
1
2
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
2
-
-
-
-
-
-
-
1
2
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
326396
Vita
Baker's yeast uridine nucleosi ...
Saccharomyces cerevisiae
Boll. Soc. Ital. Biol. Sper.
50
1077-1083
1974
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
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-
1
-
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-
-
-
-
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-
-
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-
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-
-
-
2
-
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-
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-
-
-
-
-
-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
326397
Corradetti
Baker's yeast uridine nucleosi ...
Saccharomyces cerevisiae
Boll. Soc. Ital. Biol. Sper.
50
1070-1076
1974
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
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4168
Murray
-
Molecular weight estimations o ...
Pisum sativum
Phytochemistry
10
2645-2648
1971
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1
1
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1
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1
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2
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1
1
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1
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2
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326399
Raggi-Ranieri
Partial purification and prope ...
Saccharomyces cerevisiae
Ital. J. Biochem.
20
27-43
1971
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5
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1
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2
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1
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1
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1
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3
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1
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5
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1
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1
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1
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1
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3
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1
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326401
Achar
Purification and properties of ...
Vigna radiata var. radiata
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356-362
1967
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1
5
1
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1
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1
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1
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1
1
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1
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1
1
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1
1
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1
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5
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1
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1
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1
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1
1
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1
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1
1
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1
1
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