BRENDA - Reference to 3.2.2.3; Id = 655283

H254A

sited-directed mutagenesis, active site mutant, 30fold decreased Vmax and unaltered Km compared to the wild-type enzyme

Saccharomyces cerevisiae

additional information

construction of urh115 disruption mutant strains

Saccharomyces cerevisiae

5-fluorocytidine

noncompetitive

Saccharomyces cerevisiae

5-fluorouridine

; competitive

Saccharomyces cerevisiae

cytidine

noncompetitive

Saccharomyces cerevisiae

deoxycytidine

competitive

Saccharomyces cerevisiae

Inosine

noncompetitive

Saccharomyces cerevisiae

ribosylthymine

competitive

Saccharomyces cerevisiae

additional information

Saccharomyces cerevisiae

physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview

?

-

uridine + H2O

Saccharomyces cerevisiae

enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway

D-ribose + uracil

-

?

Saccharomyces cerevisiae

Q04179

wild-type strain MATaa and mutant derivatives, gene URH1

-

uridine + H2O = D-ribose + uracil

substrate binding site, active site structure, His254 is important, catalytic mechanism

Saccharomyces cerevisiae

5-fluorocytidine + H2O

-

655283

Saccharomyces cerevisiae

D-ribose + 5-fluorocytosine

-

?

5-fluorouridine + H2O

-

655283

Saccharomyces cerevisiae

D-ribose + 5-fluorouracil

-

?

cytidine + H2O

-

655283

Saccharomyces cerevisiae

D-ribose + cytosine

-

?

deoxycytidine + H2O

-

655283

Saccharomyces cerevisiae

D-deoxyribose + cytosine

-

?

additional information

physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview

655283

Saccharomyces cerevisiae

?

-

additional information

substrate specificity, enzyme possesses no phosphorylase activity

655283

Saccharomyces cerevisiae

?

-

uridine + H2O

-

655283

Saccharomyces cerevisiae

D-ribose + uracil

-

?

uridine + H2O

enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway

655283

Saccharomyces cerevisiae

D-ribose + uracil

-

?

Urh1p

-

Saccharomyces cerevisiae

uridine/cytidine hydrolase

-

Saccharomyces cerevisiae

37

-

assay at

Saccharomyces cerevisiae

7.5

-

assay at

Saccharomyces cerevisiae

0.6

-

5-fluorouridine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

2

-

cytidine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

2

-

ribosylthymine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3

-

5-fluorocytidine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3

-

deoxycytidine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3

-

Inosine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

H254A

sited-directed mutagenesis, active site mutant, 30fold decreased Vmax and unaltered Km compared to the wild-type enzyme

Saccharomyces cerevisiae

additional information

construction of urh115 disruption mutant strains

Saccharomyces cerevisiae

5-fluorocytidine

noncompetitive

Saccharomyces cerevisiae

5-fluorouridine

-

Saccharomyces cerevisiae

5-fluorouridine

competitive

Saccharomyces cerevisiae

cytidine

noncompetitive

Saccharomyces cerevisiae

deoxycytidine

competitive

Saccharomyces cerevisiae

Inosine

noncompetitive

Saccharomyces cerevisiae

ribosylthymine

competitive

Saccharomyces cerevisiae

0.6

-

5-fluorouridine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

2

-

cytidine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

2

-

ribosylthymine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3

-

5-fluorocytidine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3

-

deoxycytidine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3

-

Inosine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

additional information

Saccharomyces cerevisiae

physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview

?

-

uridine + H2O

Saccharomyces cerevisiae

enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway

D-ribose + uracil

-

?

5-fluorocytidine + H2O

-

655283

Saccharomyces cerevisiae

D-ribose + 5-fluorocytosine

-

?

5-fluorouridine + H2O

-

655283

Saccharomyces cerevisiae

D-ribose + 5-fluorouracil

-

?

cytidine + H2O

-

655283

Saccharomyces cerevisiae

D-ribose + cytosine

-

?

deoxycytidine + H2O

-

655283

Saccharomyces cerevisiae

D-deoxyribose + cytosine

-

?

additional information

physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview

655283

Saccharomyces cerevisiae

?

-

additional information

substrate specificity, enzyme possesses no phosphorylase activity

655283

Saccharomyces cerevisiae

?

-

uridine + H2O

-

655283

Saccharomyces cerevisiae

D-ribose + uracil

-

?

uridine + H2O

enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway

655283

Saccharomyces cerevisiae

D-ribose + uracil

-

?

37

-

assay at

Saccharomyces cerevisiae

7.5

-

assay at

Saccharomyces cerevisiae

751844

Baccolini

AMP and GMP catabolism in Ara ...

Arabidopsis thaliana

Plant Cell

31

734-751

2019

-

4

-

3

-

1

-

731361

Arivett

Characterization of inosine-ur ...

Escherichia coli

Biochim. Biophys. Acta

1844

656-662

2014

-

1

-

6

-

3

-

2

-

1

-

7

1

2

1

-

6

1

-

1

-

6

-

3

-

1

-

7

1

-

6

1

-

6

731446

Shea

Identification of proton-pump ...

Trichomonas vaginalis

Bioorg. Med. Chem. Lett.

24

1080-1084

2014

-

4

-

3

-

2

1

-

1

-

3

-

3

4

-

1

-

1

-

732656

Kopecna

Structure and function of nucl ...

Physcomitrella patens, Zea mays

Plant Physiol.

163

1568-1583

2013

-

2

-

1

-

9

-

3

-

3

-

19

-

3

2

-

8

2

-

3

-

1

-

9

-

3

-

19

-

3

-

8

3

-

3

4

-

8

716542

Jung

Arabidopsis nucleoside hydrola ...

Arabidopsis thaliana

Plant J.

65

703-711

2011

-

5

-

1

-

1

-

6

-

1

-

5

-

1

-

6

-

2

-

698901

Belenky

Nicotinamide riboside and nico ...

Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4742

J. Biol. Chem.

284

158-164

2009

-

1

-

2

4

-

1

-

28

-

8

-

2

-

4

-

1

-

2

-

4

-

1

-

8

-

4

-

700741

Jung

Uridine-ribohydrolase is a key ...

Arabidopsis thaliana, Oryza sativa

Plant Cell

21

876-891

2009

-

2

-

1

-

3

-

2

-

5

1

-

2

-

4

-

2

-

1

-

3

-

5

1

-

2

-

1

-

710272

Mach

-

Uridine ribohydrolase and the ...

Arabidopsis thaliana

Plant Cell

21

0000

2009

-

1

-

1

-

5

-

1

-

2

-

1

-

5

-

1

-

683968

Katahira

-

Dual function of pyrimidine me ...

Solanum tuberosum

Physiol. Plant.

127

38-43

2006

-

1

-

2

-

2

-

638539

Mitterbauer

Saccharomyces cerevisiae URH1 ...

Crithidia fasciculata, Homo sapiens, Saccharomyces cerevisiae

Appl. Environ. Microbiol.

68

1336-1343

2002

-

1

3

-

1

-

7

-

5

-

8

-

6

1

-

1

3

-

1

-

7

-

8

-

1

-

655283

Kurtz

The URH1 uridine ribohydrolase ...

Saccharomyces cerevisiae

Curr. Genet.

41

132-141

2002

-

2

-

6

-

2

-

4

-

1

-

8

-

2

1

-

1

-

6

-

2

-

7

6

-

2

-

8

-

1

-

1

-

326402

Kurtz

New insights into the pyrimidi ...

Saccharomyces cerevisiae

Curr. Genet.

36

130-136

1999

-

1

-

2

-

3

-

1

-

3

-

326393

Lee

-

Uridine nucleosidase from malt ...

Hordeum vulgare

J. Am. Soc. Brew. Chem.

45

131-135

1987

-

2

1

-

2

-

1

-

1

-

1

-

1

-

1

-

2

-

1

-

2

-

1

-

2

-

1

-

1

-

1

-

2

-

1

-

326394

Magni

Uridine nucleosidase from yeas ...

Saccharomyces cerevisiae

Methods Enzymol.

51

290-296

1978

-

14

2

-

1

2

-

2

-

1

2

1

-

1

-

14

-

2

-

1

2

-

1

2

1

-

1

-

326395

Magni

Baker's yeast uridine nucleosi ...

Saccharomyces cerevisiae

Biochem. Biophys. Res. Commun.

69

724-730

1976

-

9

-

1

-

2

-

1

-

1

-

9

-

1

-

1

-

1

-

326398

Magni

Bakers yeast uridine nucleosid ...

Saccharomyces cerevisiae

J. Biol. Chem.

250

9-13

1975

-

4

2

-

2

-

2

-

1

2

1

-

1

-

4

-

2

-

2

-

1

2

1

-

1

-

326396

Vita

Baker's yeast uridine nucleosi ...

Saccharomyces cerevisiae

Boll. Soc. Ital. Biol. Sper.

50

1077-1083

1974

-

2

-

2

-

1

-

2

-

1

-

326397

Corradetti

Baker's yeast uridine nucleosi ...

Saccharomyces cerevisiae

Boll. Soc. Ital. Biol. Sper.

50

1070-1076

1974

-

2

-

1

-

1

-

1

-

1

-

4168

Murray

-

Molecular weight estimations o ...

Pisum sativum

Phytochemistry

10

2645-2648

1971

-

1

-

1

-

1

-

2

-

1

-

1

-

2

-

326399

Raggi-Ranieri

Partial purification and prope ...

Saccharomyces cerevisiae

Ital. J. Biochem.

20

27-43

1971

-

5

-

1

-

2

-

1

-

1

-

1

-

3

-

1

-

5

-

1

-

1

-

1

-

1

-

3

-

1

-

326401

Achar

Purification and properties of ...

Vigna radiata var. radiata

Arch. Biochem. Biophys.

119

356-362

1967

-

1

5

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

5

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

The URH1 uridine ribohydrolase of Saccharomyces cerevisiae

Data extracted from this reference: