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Literature summary for 3.2.2.3 extracted from

  • Mitterbauer, R.; Karl, T.; Adam, G.
    Saccharomyces cerevisiae URH1 (encoding uridine-cytidine N-ribohydrolase): functional complementation by a nucleoside hydrolase from a protozoan parasite and by a mammalian uridine phosphorylase (2002), Appl. Environ. Microbiol., 68, 1336-1343.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.3

Application

Application Comment Organism
pharmacology Saccharomyces cerevisiae enzyme deficient mutant strains expressing protozoan enzymes could be useful tools in drug screening for specific inhibitors against the pathogenic parasites Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
construction of enzyme overexpressing strains Saccharomyces cerevisiae
expression in a Saccharomyces mutant deficient in uridine-cytidine N-ribohydrolase restores the enzyme activity Homo sapiens
expression in a Saccharomyces mutant deficient in uridine-cytidine N-ribohydrolase restores the enzyme activity Crithidia fasciculata

Protein Variants

Protein Variants Comment Organism
additional information expression of uridine-cytidine N-ribohydrolase from protozoan parasite Crithidia fasciculata in a Saccharomyces mutant deficient restores the enzyme activity, as well as expression of the human enzyme in the yeast mutant Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytidine + H2O Homo sapiens
-
 D-ribose + cytosine
-
 ?
cytidine + H2O Saccharomyces cerevisiae
-
 D-ribose + cytosine
-
 ?
cytidine + H2O Crithidia fasciculata
-
 D-ribose + cytosine
-
 ?
additional information Saccharomyces cerevisiae key enzyme of the pyrimidine salvage pathway, overview, enzyme is required for growth of mutants deficient in uridine kinase activity ?
-
 ?
uridine + H2O Homo sapiens
-
 D-ribose + uracil
-
 ?
uridine + H2O Saccharomyces cerevisiae
-
 D-ribose + uracil
-
 ?
uridine + H2O Crithidia fasciculata
-
 D-ribose + uracil
-
 ?

Organism

Organism UniProt Comment Textmining
Crithidia fasciculata Q27546 protozoan parasite
-
Homo sapiens
-
-
-
Saccharomyces cerevisiae
-
 enzyme URH1 or Urh1p, gene URH1, encoded in the ORF YDR400w
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytidine + H2O
-
 Homo sapiens D-ribose + cytosine
-
 ?
cytidine + H2O
-
 Saccharomyces cerevisiae D-ribose + cytosine
-
 ?
cytidine + H2O
-
 Crithidia fasciculata D-ribose + cytosine
-
 ?
additional information key enzyme of the pyrimidine salvage pathway, overview, enzyme is required for growth of mutants deficient in uridine kinase activity Saccharomyces cerevisiae ?
-
 ?
additional information inosine, adenosine, guanosine, and thymidine are poor substrates Saccharomyces cerevisiae ?
-
 ?
uridine + H2O
-
 Homo sapiens D-ribose + uracil
-
 ?
uridine + H2O
-
 Saccharomyces cerevisiae D-ribose + uracil
-
 ?
uridine + H2O
-
 Crithidia fasciculata D-ribose + uracil
-
 ?

Synonyms

Synonyms Comment Organism
URH1
-
 Saccharomyces cerevisiae
Urh1p
-
 Saccharomyces cerevisiae
uridine hydrolase
-
 Saccharomyces cerevisiae
uridine-cytidine N-ribohydrolase
-
 Homo sapiens
uridine-cytidine N-ribohydrolase
-
 Saccharomyces cerevisiae
uridine-cytidine N-ribohydrolase
-
 Crithidia fasciculata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae