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Information on EC 3.2.1.122 - maltose-6'-phosphate glucosidase
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EC Tree
3.2.1.122 maltose-6'-phosphate glucosidaseIUBMB Comments
Hydrolyses a variety of 6-phospho-alpha-D-glucosides, including alpha-maltose 6'-phosphate, alpha,alpha-trehalose 6-phosphate, sucrose 6-phosphate and p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate (as a chromogenic substrate). The enzyme is activated by FeII, MnII, CoII and NiII. It is rapidly inactivated in air.
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The enzyme appears in viruses and cellular organisms
Synonyms
6-phosphoryl-o-alpha-d-glucopyranosyl:phosphoglucohydrolase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
6-phospho-alpha-D-glucosidase
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-
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6-phospho-alpha-glucosidase
6-phosphoryl-O-alpha-D-glucopyranosyl:6phosphoglucohydrolase
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6-phosphoryl-O-alpha-D-glucopyranosyl:phosphoglucohydrolase
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GlvA
MalH
maltose 6P hydrolase
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-
-
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maltose-6'-phosphate phosphatase
mapP
phospho-alpha-glucosidase
additional information
6-phospho-alpha-glucosidase
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-
-
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phospho-alpha-glucosidase
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-
-
-
additional information
belongs to familiy 4 of the glycosylhydrolase superfamily
additional information
belongs to familiy 4 of the glycosylhydrolase superfamily
additional information
belongs to familiy 4 of the glycosylhydrolase superfamily
additional information
belongs to familiy 4 of the glycosylhydrolase superfamily
additional information
belongs to familiy 4 of the glycosylhydrolase superfamily
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
alpha-maltose 6'-phosphate + H2O = D-glucose + D-glucose 6-phosphate
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
maltose-6'-phosphate 6-phosphoglucohydrolase
Hydrolyses a variety of 6-phospho-alpha-D-glucosides, including alpha-maltose 6'-phosphate, alpha,alpha-trehalose 6-phosphate, sucrose 6-phosphate and p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate (as a chromogenic substrate). The enzyme is activated by FeII, MnII, CoII and NiII. It is rapidly inactivated in air.
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CAS REGISTRY NUMBER
COMMENTARY
98445-08-0
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside + H2O
3,4-dinitrophenol + alpha-D-glucose 6-phosphate
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-
-
-
?
4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + 4-methylumbelliferone
4-nitrophenyl 6-phospho-alpha-D-glucoside + H2O
4-nitrophenol + alpha-D-glucose
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-
-
-
?
4-nitrophenyl 6-phospho-beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose 6-phosphate
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-
?
6-phospho-beta-D-glucose + H2O
phosphate + D-glucose
substrate analog
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?
isomaltose 6'-phosphate + H2O
alpha-D-glucopyranosyl 6-phosphate + D-glucose
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-
?
maltitol 6-phosphate + H2O
alpha-D-glucopyranosyl 6-phosphate + D-glucitol
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?
maltose 6,6'-bisphosphate + H2O
D-glucose 6-phosphate + D-fructose
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6.0% activity compared to maltose 6'-phosphate
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-
?
methyl 6-phospho-alpha-D-glucoside + H2O
methanol + alpha-D-glucose 6-phosphate
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?
methyl-alpha-D-glucopyranoside + H2O
methanol + alpha-D-glucose
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?
p-nitrophenyl alpha-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + alpha-D-glucose 6-phosphate
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?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
beta-D-glucose 6-phosphate + p-nitrophenol
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-
?
phenyl 6-phospho-alpha-D-glucoside + H2O
phenol + alpha-D-glucose 6-phosphate
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-
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?
sucrose 6-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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hydrolysis to yield D-glucose 6-phosphate and aglycone moiety in 1:1 molar ratio
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?
4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + 4-methylumbelliferone
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-
-
?
4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + 4-methylumbelliferone
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fluorescent product
?
4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + 4-methylumbelliferone
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fluorescent product
?
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose + H2O
?
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?
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose + H2O
?
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?
esculin 6-phosphate + H2O
?
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0.6% activity compared to maltose 6'-phosphate
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?
esculin 6-phosphate + H2O
?
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0.6% activity compared to maltose 6'-phosphate
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-
?
leucrose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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-
-
?
leucrose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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18.8% activity compared to maltose 6'-phosphate
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?
leucrose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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?
leucrose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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?
maltose 6'-phosphate + H2O
D-glucose + D-glucose 6-phosphate
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?
maltose 6'-phosphate + H2O
D-glucose + D-glucose 6-phosphate
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100% activity
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?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system
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?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system
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?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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broad substrate specificity
molar ratio is 1:1
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
broad substrate specificity
molar ratio is 1:1
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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in the pathway of maltose fermentation
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?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
broad substrate specificity
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?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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involved in maltose glycolysis, enzyme is induced by maltose
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?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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involved in maltose glycolysis, enzyme is induced by maltose
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?
maltulose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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?
maltulose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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8.1% activity compared to maltose 6'-phosphate
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?
maltulose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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?
maltulose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
MalH and PagL, Cys-169 and Asp-170 are essential for hydrolysis
yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
only substrate
yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
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molar ratio is 1:1, yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
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yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
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yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
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yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
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yellow product
?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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0.9% activity compared to maltose 6'-phosphate
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?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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0.9% activity compared to maltose 6'-phosphate
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?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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?
trehalose 6-phosphate + H2O
?
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hydrolysis to yield D-glucose 6-phosphate and aglycone moiety in 1:1 molar ratio
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?
trehalulose 6'-phosphate + H2O
alpha-D-glucose 6-phosphate + D-fructose
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?
trehalulose 6'-phosphate + H2O
alpha-D-glucose 6-phosphate + D-fructose
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2.1% activity compared to maltose 6'-phosphate
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?
trehalulose 6'-phosphate + H2O
alpha-D-glucose 6-phosphate + D-fructose
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?
trehalulose 6'-phosphate + H2O
alpha-D-glucose 6-phosphate + D-fructose
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?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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85% activity compared to maltose 6'-phosphate
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?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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85% activity compared to maltose 6'-phosphate
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?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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molar ratio is 1:1
?
additional information
?
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6-phospho-alpha-glycosides are the preferred substrates of GlvA
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?
additional information
?
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not: methyl-beta-glucose 6-phosphate
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?
additional information
?
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not: methyl-beta-glucose 6-phosphate
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?
additional information
?
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not: cellobiose 6-phosphate, gentiobiose 6-phosphate
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?
additional information
?
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not: cellobiose 6-phosphate, gentiobiose 6-phosphate
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?
additional information
?
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only MalH catalyzes the hydrolysis of disaccharide 6-phosphates formed via the phosphoenolpyruvate-dependent:sugar phosphotransferase system, not PagL
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?
additional information
?
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only MalH catalyzes the hydrolysis of disaccharide 6-phosphates formed via the phosphoenolpyruvate-dependent:sugar phosphotransferase system, not PagL
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?
additional information
?
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substrate specificity, not: sucrose 6-phosphate
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?
additional information
?
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substrate specificity, not: sucrose 6-phosphate
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?
additional information
?
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MalH participates in dissimilation of maltose and sucrose isomers, PagL not
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?
additional information
?
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MalH participates in dissimilation of maltose and sucrose isomers, PagL not
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?
additional information
?
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4-nitrophenyl-alpha-D-glucopyranoside 6-phosphate, cellobiose 6'-phosphate, thiocellobiose 6'-phosphate, cellobiitol 6'-phosphate, beta-methylglucoside 6-phosphate, salicin 6-phosphate, laminaribose 6'-phosphate, gentiobiose 6'-phosphate, arbutin 6-phosphate, sucrose 6-phosphate, D-glucose 6-phosphate, D-fructose 6-phosphate and bet-methylglucoside 6-phosphate are not dephosphorylated by the enzyme
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?
additional information
?
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the enzyme preferably dephosphorylates disaccharides containing an O-alpha-glycosyl linkage
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?
additional information
?
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4-nitrophenyl-alpha-D-glucopyranoside 6-phosphate, cellobiose 6'-phosphate, thiocellobiose 6'-phosphate, cellobiitol 6'-phosphate, beta-methylglucoside 6-phosphate, salicin 6-phosphate, laminaribose 6'-phosphate, gentiobiose 6'-phosphate, arbutin 6-phosphate, sucrose 6-phosphate, D-glucose 6-phosphate, D-fructose 6-phosphate and bet-methylglucoside 6-phosphate are not dephosphorylated by the enzyme
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?
additional information
?
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the enzyme preferably dephosphorylates disaccharides containing an O-alpha-glycosyl linkage
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?
additional information
?
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not: cellobiose 6-phosphate, gentiobiose 6-phosphate
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?
additional information
?
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not: D-glucose, D-fructose, non-phosphorylated sucrose isomers
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?
additional information
?
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not: nonphosphorylated alpha- and beta-glycosides, p-nitrophenyl-beta-D-glucopyranoside 6-phosphate, p-nitrophenyl-alpha-D-mannopyranoside 6-phosphate, very poor substrate: p-nitrophenyl-alpha-D-galactopyranoside 6-phosphate
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-
?
additional information
?
-
substrate specificity, not: sucrose 6-phosphate
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-
?
additional information
?
-
substrate specificity, not: sucrose 6-phosphate
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-
?
additional information
?
-
substrate specificity, not: sucrose 6-phosphate
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?
additional information
?
-
AglB and AglA, an enzyme II(CB) component of the PEP:PTS, facilitate the phosphorylative translocation and subsequent cleavage of phosphorylated isomers of sucrose and related alpha-glycosides
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-
?
additional information
?
-
sucrose 6'-phosphate is not a substrate for AglB
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
maltose 6'-phosphate + H2O
D-glucose + D-glucose 6-phosphate
-
-
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
in the pathway of maltose fermentation
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
involved in maltose glycolysis, enzyme is induced by maltose
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
involved in maltose glycolysis, enzyme is induced by maltose
-
?
additional information
?
-
MalH participates in dissimilation of maltose and sucrose isomers, PagL not
-
-
?
additional information
?
-
MalH participates in dissimilation of maltose and sucrose isomers, PagL not
-
-
?
additional information
?
-
AglB and AglA, an enzyme II(CB) component of the PEP:PTS, facilitate the phosphorylative translocation and subsequent cleavage of phosphorylated isomers of sucrose and related alpha-glycosides
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
dependent on NAD+, the glycosidic bond hydrolysis is initiated by oxidation of the glucopyranose ring at the 3-position to a keto group by the NAD+ molecule bound to the active site
additional information
requires a divalent metal ion, e.g. Mn2+
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Co2+
Fe2+
Mn2+
Ni2+
additional information
Mn2+
-
requirement, permits formation of the active tetramer of GlvA from its inactive dimeric state
additional information
requires a divalent metal ion, e.g. Mn2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
differential expression of MalH and PagL during growth on various sugars, enzyme induction, nucleotide specificity for activation of MalH and PagL
-
additional information
differential expression of MalH and PagL during growth on various sugars, enzyme induction, nucleotide specificity for activation of MalH and PagL
-
additional information
MalH is induced during growth on the sucrose isomers trehalulose, turanose, maltulose, leucrose and palatinose, but not on sucrose
-
additional information
-
growth on alpha-glycosides, such as maltose, alpha-methylglycoside, trehalose, turanose and palatinose, increases dramatically enzyme synthesis
-
additional information
growth on alpha-glycosides, such as maltose, alpha-methylglycoside, trehalose, turanose and palatinose, increases dramatically enzyme synthesis
-
additional information
-
growth on alpha-glycosides, such as maltose, alpha-methylglycoside, trehalose, turanose and palatinose, increases dramatically enzyme synthesis
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.004
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside
-
at 37°C in Tris-HCl buffer, pH 8.4
0.012
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside
-
at 37°C in HEPES buffer, pH 7.5
0.82
3-O-(6-O-phosphono-alpha-D-glucopyranosyl)-alpha-D-fructofuranose
pH 7, 37°C
1.68
3-O-(6-O-phosphono-alpha-D-glucopyranosyl)-alpha-D-fructofuranose
pH 7.5
0.014
4-nitrophenyl 6-phospho-alpha-D-glucoside
-
at 37°C in Tris-HCl buffer, pH 8.4
0.052
4-nitrophenyl 6-phospho-alpha-D-glucoside
-
at 37°C in HEPES buffer, pH 7.5
2.42
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose
pH 7.5
2.47
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose
pH 7, 37°C
0.5
methyl 6-phospho-alpha-D-glucoside