Literature summary for 3.2.1.122 extracted from

Bouma, C.L.; Reizer, J.; Reizer, A.; Robrish, S.A.; Thompson, J.
6-Phospho-alpha-D-glucosidase from Fusobacterium mortiferum: Cloning, expression, and assignment to family 4 of the glycosylhydrolases (1997), J. Bacteriol., 179, 4129-4137.

Search for more literature for 3.2.1.122

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	growth on alpha-glycosides, such as maltose, alpha-methylglycoside, trehalose, turanose and palatinose, increases dramatically enzyme synthesis	Fusobacterium mortiferum

Cloned(Commentary)

Cloned (Comment)	Organism
malH gene encodes maltose 6P hydrolase, a 441 residues protein, and is cloned, sequenced and expressed in Escherichia coli XLOLR	Fusobacterium mortiferum

General Stability

General Stability	Organism
dialysis and storage overnight results in 90% loss of activity	Fusobacterium mortiferum

Inhibitors

Inhibitors	Comment	Organism	Structure
air	exposure to air rapidly inactivates	Fusobacterium mortiferum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	requires Fe2+, Mn2+, Co2+ or Ni2+	Fusobacterium mortiferum
Fe2+	requires Fe2+, Mn2+, Co2+ or Ni2+	Fusobacterium mortiferum
Mn2+	requires Fe2+, Mn2+, Co2+ or Ni2+	Fusobacterium mortiferum
Ni2+	requires Fe2+, Mn2+, Co2+ or Ni2+	Fusobacterium mortiferum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
49000	-	x * 49000, SDS-PAGE	Fusobacterium mortiferum
49718	-	x * 49718, calculated from the amino acid sequence	Fusobacterium mortiferum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Fusobacterium mortiferum	carbohydrate metabolism	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Fusobacterium mortiferum	O06901	strain ATCC 25557, MalH	-

Oxidation Stability

Oxidation Stability	Organism
O2-sensitive enzyme, rapidly inactivated by exposure to air	Fusobacterium mortiferum

Purification (Commentary)

Purification (Comment)	Organism
-	Fusobacterium mortiferum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Fusobacterium mortiferum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O	-	Fusobacterium mortiferum	alpha-D-glucose 6-phosphate + 4-methylumbelliferone	fluorescent product	?
maltose 6'-phosphate + H2O	broad substrate specificity	Fusobacterium mortiferum	D-glucose 6-phosphate + D-glucose	molar ratio is 1:1	?
additional information	carbohydrate metabolism	Fusobacterium mortiferum	?	-	?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O	-	Fusobacterium mortiferum	alpha-D-glucose 6-phosphate + p-nitrophenol	yellow product	?
trehalose 6-phosphate + H2O	-	Fusobacterium mortiferum	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 49000, SDS-PAGE	Fusobacterium mortiferum
?	x * 49718, calculated from the amino acid sequence	Fusobacterium mortiferum

Synonyms

Synonyms	Comment	Organism
MalH	-	Fusobacterium mortiferum
More	belongs to familiy 4 of the glycosylhydrolase superfamily	Fusobacterium mortiferum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Fusobacterium mortiferum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Fusobacterium mortiferum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Fusobacterium mortiferum	analytical isoelectric focusing	-	4.9
Fusobacterium mortiferum	amino acid sequence	-	5.5

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Release 2023.1 (January 2023)