Cloned (Comment) | Organism |
---|---|
aglB gene is cloned, sequenced and expressed in Escherichia coli TOP 10 | Klebsiella pneumoniae |
structural organization of the putative alpha-glycoside operon | Klebsiella pneumoniae |
General Stability | Organism |
---|---|
inclusion of 0.1 mM NAD+ and 1 mM Mn2+ in all buffers stabilizes during purification | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate | pH 7.5 | Klebsiella pneumoniae | |
0.82 | - |
maltitol 6-phosphate | pH 7.5 | Klebsiella pneumoniae | |
1.16 | - |
trehalose 6-phosphate | pH 7.5 | Klebsiella pneumoniae | |
1.2 | - |
maltulose 6'-phosphate | pH 7.5 | Klebsiella pneumoniae | |
1.23 | - |
trehalulose 6'-phosphate | pH 7.5 | Klebsiella pneumoniae | |
1.68 | - |
3-O-(6-O-phosphono-alpha-D-glucopyranosyl)-alpha-D-fructofuranose | pH 7.5 | Klebsiella pneumoniae | |
2.42 | - |
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose | pH 7.5 | Klebsiella pneumoniae | |
3.08 | - |
maltose 6'-phosphate | pH 7.5 | Klebsiella pneumoniae | |
4.48 | - |
isomaltose 6'-phosphate | pH 7.5 | Klebsiella pneumoniae | |
5.63 | - |
leucrose 6'-phosphate | pH 7.5 | Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | requires Mn2+, Co2+ or Ni2+ | Klebsiella pneumoniae | |
Mn2+ | requires Mn2+, Co2+ or Ni2+ | Klebsiella pneumoniae | |
additional information | metal-dependent | Klebsiella pneumoniae | |
additional information | not activated by Mg2+, Zn2+ or Ca2+ | Klebsiella pneumoniae | |
Ni2+ | requires Mn2+, Co2+ or Ni2+ | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49256 | - |
2 * 49256, calculated from the amino acid sequence | Klebsiella pneumoniae |
50000 | - |
2 * 50000, SDS-PAGE and Western blot analysis | Klebsiella pneumoniae |
100000 | - |
about, gel filtration | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Klebsiella pneumoniae | AglB and AglA, an enzyme II(CB) component of the PEP:PTS, facilitate the phosphorylative translocation and subsequent cleavage of phosphorylated isomers of sucrose and related alpha-glycosides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | Q9AGA6 | strain ATCC 23357, AglB | - |
Purification (Comment) | Organism |
---|---|
recombinant AglB expressed in Escherichia coli TOP 10, 3.4fold | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Klebsiella pneumoniae |
4.15 | - |
pH 7.5, recombinant AglB, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose + H2O | - |
Klebsiella pneumoniae | ? | - |
? | |
isomaltose 6'-phosphate + H2O | - |
Klebsiella pneumoniae | alpha-D-glucopyranosyl 6-phosphate + D-glucose | - |
? | |
leucrose 6'-phosphate + H2O | - |
Klebsiella pneumoniae | D-glucose 6-phosphate + D-fructose | - |
? | |
maltitol 6-phosphate + H2O | - |
Klebsiella pneumoniae | alpha-D-glucopyranosyl 6-phosphate + D-glucitol | - |
? | |
maltose 6'-phosphate + H2O | broad substrate specificity | Klebsiella pneumoniae | D-glucose 6-phosphate + D-glucose | - |
? | |
maltulose 6'-phosphate + H2O | - |
Klebsiella pneumoniae | D-glucose 6-phosphate + D-fructose | - |
? | |
additional information | substrate specificity, not: sucrose 6-phosphate | Klebsiella pneumoniae | ? | - |
? | |
additional information | AglB and AglA, an enzyme II(CB) component of the PEP:PTS, facilitate the phosphorylative translocation and subsequent cleavage of phosphorylated isomers of sucrose and related alpha-glycosides | Klebsiella pneumoniae | ? | - |
? | |
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O | - |
Klebsiella pneumoniae | alpha-D-glucose 6-phosphate + p-nitrophenol | yellow product | ? | |
palatinose 6'-phosphate + H2O | - |
Klebsiella pneumoniae | D-glucose 6-phosphate + D-fructose | - |
? | |
trehalose 6-phosphate + H2O | - |
Klebsiella pneumoniae | ? | - |
? | |
trehalulose 6'-phosphate + H2O | - |
Klebsiella pneumoniae | alpha-D-glucose 6-phosphate + D-fructose | - |
? | |
turanose 6'-phosphate + H2O | - |
Klebsiella pneumoniae | D-glucose 6-phosphate + D-fructose | molar ratio is 1:1 | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | catalytically active homodimer | Klebsiella pneumoniae |
dimer | 2 * 49256, calculated from the amino acid sequence | Klebsiella pneumoniae |
dimer | 2 * 50000, SDS-PAGE and Western blot analysis | Klebsiella pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
AglB | - |
Klebsiella pneumoniae |
More | belongs to familiy 4 of the glycosylhydrolase superfamily | Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | - |
in 0.1 M Tris-HCl or HEPES buffer, pH 7.5, 0.1 mM NAD+, 1 mM Mn2+ | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NAD+-dependent | Klebsiella pneumoniae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Klebsiella pneumoniae | 2 species with pI values of 5.4 and 5.6, analytical isoelectric focusing | - |
5.4 |
Klebsiella pneumoniae | theoretical pI deduced from the amino acid composition | - |
5.69 |