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Literature summary for 3.2.1.122 extracted from

  • Thompson, J.; Robrish, S.A.; Immel, S.; Lichtenthaler, F.W.; Hall, B.G.; Pikis, A.
    Metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae. Participation and properties of sucrose-6-phosphate hydrolase and phospho-alpha-glucosidase (2001), J. Biol. Chem., 276, 37415-37425.
    View publication on PubMed
Search for more literature for 3.2.1.122

Cloned(Commentary)

Cloned (Comment) Organism
aglB gene is cloned, sequenced and expressed in Escherichia coli TOP 10 Klebsiella pneumoniae
structural organization of the putative alpha-glycoside operon Klebsiella pneumoniae

General Stability

General Stability Organism
inclusion of 0.1 mM NAD+ and 1 mM Mn2+ in all buffers stabilizes during purification Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate pH 7.5 Klebsiella pneumoniae
0.82
-
 maltitol 6-phosphate pH 7.5 Klebsiella pneumoniae
1.16
-
 trehalose 6-phosphate pH 7.5 Klebsiella pneumoniae
1.2
-
 maltulose 6'-phosphate pH 7.5 Klebsiella pneumoniae
1.23
-
 trehalulose 6'-phosphate pH 7.5 Klebsiella pneumoniae
1.68
-
 3-O-(6-O-phosphono-alpha-D-glucopyranosyl)-alpha-D-fructofuranose pH 7.5 Klebsiella pneumoniae
2.42
-
 6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose pH 7.5 Klebsiella pneumoniae
3.08
-
 maltose 6'-phosphate pH 7.5 Klebsiella pneumoniae
4.48
-
 isomaltose 6'-phosphate pH 7.5 Klebsiella pneumoniae
5.63
-
 leucrose 6'-phosphate pH 7.5 Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ requires Mn2+, Co2+ or Ni2+ Klebsiella pneumoniae
Mn2+ requires Mn2+, Co2+ or Ni2+ Klebsiella pneumoniae
additional information metal-dependent Klebsiella pneumoniae
additional information not activated by Mg2+, Zn2+ or Ca2+ Klebsiella pneumoniae
Ni2+ requires Mn2+, Co2+ or Ni2+ Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49256
-
 2 * 49256, calculated from the amino acid sequence Klebsiella pneumoniae
50000
-
 2 * 50000, SDS-PAGE and Western blot analysis Klebsiella pneumoniae
100000
-
 about, gel filtration Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Klebsiella pneumoniae AglB and AglA, an enzyme II(CB) component of the PEP:PTS, facilitate the phosphorylative translocation and subsequent cleavage of phosphorylated isomers of sucrose and related alpha-glycosides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae Q9AGA6 strain ATCC 23357, AglB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant AglB expressed in Escherichia coli TOP 10, 3.4fold Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Klebsiella pneumoniae
4.15
-
 pH 7.5, recombinant AglB, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose + H2O
-
 Klebsiella pneumoniae ?
-
 ?
isomaltose 6'-phosphate + H2O
-
 Klebsiella pneumoniae alpha-D-glucopyranosyl 6-phosphate + D-glucose
-
 ?
leucrose 6'-phosphate + H2O
-
 Klebsiella pneumoniae D-glucose 6-phosphate + D-fructose
-
 ?
maltitol 6-phosphate + H2O
-
 Klebsiella pneumoniae alpha-D-glucopyranosyl 6-phosphate + D-glucitol
-
 ?
maltose 6'-phosphate + H2O broad substrate specificity Klebsiella pneumoniae D-glucose 6-phosphate + D-glucose
-
 ?
maltulose 6'-phosphate + H2O
-
 Klebsiella pneumoniae D-glucose 6-phosphate + D-fructose
-
 ?
additional information substrate specificity, not: sucrose 6-phosphate Klebsiella pneumoniae ?
-
 ?
additional information AglB and AglA, an enzyme II(CB) component of the PEP:PTS, facilitate the phosphorylative translocation and subsequent cleavage of phosphorylated isomers of sucrose and related alpha-glycosides Klebsiella pneumoniae ?
-
 ?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
-
 Klebsiella pneumoniae alpha-D-glucose 6-phosphate + p-nitrophenol yellow product ?
palatinose 6'-phosphate + H2O
-
 Klebsiella pneumoniae D-glucose 6-phosphate + D-fructose
-
 ?
trehalose 6-phosphate + H2O
-
 Klebsiella pneumoniae ?
-
 ?
trehalulose 6'-phosphate + H2O
-
 Klebsiella pneumoniae alpha-D-glucose 6-phosphate + D-fructose
-
 ?
turanose 6'-phosphate + H2O
-
 Klebsiella pneumoniae D-glucose 6-phosphate + D-fructose molar ratio is 1:1 ?

Subunits

Subunits Comment Organism
dimer catalytically active homodimer Klebsiella pneumoniae
dimer 2 * 49256, calculated from the amino acid sequence Klebsiella pneumoniae
dimer 2 * 50000, SDS-PAGE and Western blot analysis Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
AglB
-
 Klebsiella pneumoniae
More belongs to familiy 4 of the glycosylhydrolase superfamily Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
36
-
 in 0.1 M Tris-HCl or HEPES buffer, pH 7.5, 0.1 mM NAD+, 1 mM Mn2+ Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+-dependent Klebsiella pneumoniae

pI Value

Organism Comment pI Value Maximum pI Value
Klebsiella pneumoniae 2 species with pI values of 5.4 and 5.6, analytical isoelectric focusing
-
 5.4
Klebsiella pneumoniae theoretical pI deduced from the amino acid composition
-
 5.69