Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | growth on alpha-glycosides, such as maltose, alpha-methylglycoside, trehalose, turanose and palatinose, increases dramatically enzyme synthesis | Fusobacterium mortiferum |
Cloned (Comment) | Organism |
---|---|
gene encoding maltose 6P hydrolase, a 441 residues protein, is cloned, sequenced and actively expressed in Escherichia coli | Fusobacterium mortiferum |
General Stability | Organism |
---|---|
dialysis and storage overnight results in 90% loss of activity | Fusobacterium mortiferum |
freezing and thawing from -20°C retains 50% of initial activity | Fusobacterium mortiferum |
inclusion of dithiothreitol and Mn2+ in chromatography buffers stabilizes during purification | Fusobacterium mortiferum |
purification by Rotofor isoelectric focusing inactivates | Fusobacterium mortiferum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
air | exposure to air rapidly inactivates | Fusobacterium mortiferum | |
sulfhydryl-reactive agent | rapidly inactivates | Fusobacterium mortiferum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
maltose 6'-phosphate | pH 7, 37°C | Fusobacterium mortiferum | |
2 | - |
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate | pH 7, 37°C | Fusobacterium mortiferum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Fusobacterium mortiferum | |
Fe2+ | activates | Fusobacterium mortiferum | |
Mn2+ | activates | Fusobacterium mortiferum | |
additional information | not activated by Sr2+ | Fusobacterium mortiferum | |
additional information | metal-dependent | Fusobacterium mortiferum | |
additional information | not activated by Mg2+, Zn2+ or Ca2+ | Fusobacterium mortiferum | |
Ni2+ | activates | Fusobacterium mortiferum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
x * 49000, SDS-PAGE | Fusobacterium mortiferum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose 6'-phosphate + H2O | Fusobacterium mortiferum | in the pathway of maltose fermentation | D-glucose 6-phosphate + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusobacterium mortiferum | - |
strain ATCC 25557 | - |
Oxidation Stability | Organism |
---|---|
O2-sensitive enzyme, rapidly inactivated by exposure to air | Fusobacterium mortiferum |
Purification (Comment) | Organism |
---|---|
6fold, dithiothreitol and Mn2+ is required for stabilization during purification | Fusobacterium mortiferum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.67 | - |
at room temperature, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis | Fusobacterium mortiferum |
Storage Stability | Organism |
---|---|
-20°C, 25 mM HEPES buffer, pH 7.5, 1 mM Mn2+, 1 mM dithiothreitol, several weeks, stable | Fusobacterium mortiferum |
storage overnight, 90% loss of activity | Fusobacterium mortiferum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose 6'-phosphate + H2O | broad substrate specificity | Fusobacterium mortiferum | D-glucose 6-phosphate + D-glucose | molar ratio is 1:1 | ? | |
maltose 6'-phosphate + H2O | in the pathway of maltose fermentation | Fusobacterium mortiferum | D-glucose 6-phosphate + D-glucose | - |
? | |
additional information | not: nonphosphorylated alpha- and beta-glycosides, p-nitrophenyl-beta-D-glucopyranoside 6-phosphate, p-nitrophenyl-alpha-D-mannopyranoside 6-phosphate, very poor substrate: p-nitrophenyl-alpha-D-galactopyranoside 6-phosphate | Fusobacterium mortiferum | ? | - |
? | |
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O | - |
Fusobacterium mortiferum | alpha-D-glucose 6-phosphate + p-nitrophenol | molar ratio is 1:1 | ? | |
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O | - |
Fusobacterium mortiferum | alpha-D-glucose 6-phosphate + p-nitrophenol | yellow product | ? | |
palatinose 6'-phosphate + H2O | - |
Fusobacterium mortiferum | D-glucose 6-phosphate + D-fructose | - |
? | |
sucrose 6-phosphate + H2O | hydrolysis to yield D-glucose 6-phosphate and aglycone moiety in 1:1 molar ratio | Fusobacterium mortiferum | D-glucose 6-phosphate + D-fructose | - |
? | |
trehalose 6-phosphate + H2O | hydrolysis to yield D-glucose 6-phosphate and aglycone moiety in 1:1 molar ratio | Fusobacterium mortiferum | ? | - |
? | |
turanose 6'-phosphate + H2O | - |
Fusobacterium mortiferum | D-glucose 6-phosphate + D-fructose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, SDS-PAGE | Fusobacterium mortiferum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Fusobacterium mortiferum |
40 | - |
maximum rate of p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis | Fusobacterium mortiferum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | maximum rate of p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis | Fusobacterium mortiferum |
7 | - |
assay at | Fusobacterium mortiferum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Fusobacterium mortiferum | analytical isoelectric focusing | - |
4.9 |