Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | dithiothreitol-dependent | Clostridium acetobutylicum | |
additional information | differential expression of MalH and PagL during growth on various sugars, enzyme induction, nucleotide specificity for activation of MalH and PagL | Clostridium acetobutylicum |
Cloned (Comment) | Organism |
---|---|
genes malh and pagl encoding maltose 6-phosphate hydrolase MalH and phospho-alpha-glucosidase PagL are cloned, sequenced and expressed in Escherichia coli PEP43, the 2 genes reside in separate operons, molecular organization of the operons mal and pagl | Clostridium acetobutylicum |
Protein Variants | Comment | Organism |
---|---|---|
C169S | catalytically inactive MalH mutant | Clostridium acetobutylicum |
D170N | catalytically inactive MalH mutant | Clostridium acetobutylicum |
M171V | MalH mutant with reduced activity | Clostridium acetobutylicum |
P172A | MalH mutant with reduced activity | Clostridium acetobutylicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate | pH 7, 37°C | Clostridium acetobutylicum | |
0.058 | - |
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate | pH 7, 37°C | Clostridium acetobutylicum | |
0.82 | - |
3-O-(6-O-phosphono-alpha-D-glucopyranosyl)-alpha-D-fructofuranose | pH 7, 37°C | Clostridium acetobutylicum | |
1.11 | - |
maltulose 6'-phosphate | pH 7, 37°C | Clostridium acetobutylicum | |
1.87 | - |
leucrose 6'-phosphate | pH 7, 37°C | Clostridium acetobutylicum | |
1.92 | - |
trehalulose 6'-phosphate | pH 7, 37°C | Clostridium acetobutylicum | |
1.95 | - |
maltose 6'-phosphate | pH 7, 37°C | Clostridium acetobutylicum | |
2.47 | - |
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose | pH 7, 37°C | Clostridium acetobutylicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | cofactor, requirement | Clostridium acetobutylicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49972 | - |
4 * 49972, recombinant MalH, predicted from the amino acid sequence | Clostridium acetobutylicum |
50000 | - |
4 * 50000, recombinant MalH and PagL, SDS-PAGE | Clostridium acetobutylicum |
50038 | - |
4 * 50038, recombinant MalH, electrospray ionization mass spectrometry | Clostridium acetobutylicum |
51155 | - |
4 * 51155, recombinant PagL, predicted from the amino acid sequence | Clostridium acetobutylicum |
51205 | - |
4 * 51205, recombinant PagL, electrospray ionization mass spectrometry | Clostridium acetobutylicum |
200000 | - |
about, MalH and PagL, HPLC gel filtration | Clostridium acetobutylicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Clostridium acetobutylicum | MalH participates in dissimilation of maltose and sucrose isomers, PagL not | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium acetobutylicum | Q97DP6 | strain ATCC 824, PagL, i.e. phospho-alpha-glucosidase | - |
Clostridium acetobutylicum | Q97LM4 | strain ATCC 824, MalH, i.e. maltose 6-phosphate hydrolase | - |
Purification (Comment) | Organism |
---|---|
recombinant MalH expressed in Escherichia coli PEP43 | Clostridium acetobutylicum |
recombinant PagL expressed in Escherichia coli PEP43 | Clostridium acetobutylicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.9 | - |
pH 7.5, 37°C, recombinant MalH, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis | Clostridium acetobutylicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O | - |
Clostridium acetobutylicum | alpha-D-glucose 6-phosphate + 4-methylumbelliferone | - |
? | |
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose + H2O | - |
Clostridium acetobutylicum | ? | - |
? | |
leucrose 6'-phosphate + H2O | - |
Clostridium acetobutylicum | D-glucose 6-phosphate + D-fructose | - |
? | |
maltose 6'-phosphate + H2O | - |
Clostridium acetobutylicum | D-glucose 6-phosphate + D-glucose | - |
? | |
maltulose 6'-phosphate + H2O | - |
Clostridium acetobutylicum | D-glucose 6-phosphate + D-fructose | - |
? | |
additional information | not: methyl-beta-glucose 6-phosphate | Clostridium acetobutylicum | ? | - |
? | |
additional information | not: cellobiose 6-phosphate, gentiobiose 6-phosphate | Clostridium acetobutylicum | ? | - |
? | |
additional information | only MalH catalyzes the hydrolysis of disaccharide 6-phosphates formed via the phosphoenolpyruvate-dependent:sugar phosphotransferase system, not PagL | Clostridium acetobutylicum | ? | - |
? | |
additional information | substrate specificity, not: sucrose 6-phosphate | Clostridium acetobutylicum | ? | - |
? | |
additional information | MalH participates in dissimilation of maltose and sucrose isomers, PagL not | Clostridium acetobutylicum | ? | - |
? | |
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O | MalH and PagL, Cys-169 and Asp-170 are essential for hydrolysis | Clostridium acetobutylicum | alpha-D-glucose 6-phosphate + p-nitrophenol | yellow product | ? | |
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O | only substrate | Clostridium acetobutylicum | alpha-D-glucose 6-phosphate + p-nitrophenol | yellow product | ? | |
palatinose 6'-phosphate + H2O | - |
Clostridium acetobutylicum | D-glucose 6-phosphate + D-fructose | - |
? | |
trehalulose 6'-phosphate + H2O | - |
Clostridium acetobutylicum | alpha-D-glucose 6-phosphate + D-fructose | - |
? | |
turanose 6'-phosphate + H2O | - |
Clostridium acetobutylicum | D-glucose 6-phosphate + D-fructose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 51155, recombinant PagL, predicted from the amino acid sequence | Clostridium acetobutylicum |
tetramer | 4 * 49972, recombinant MalH, predicted from the amino acid sequence | Clostridium acetobutylicum |
tetramer | 4 * 50038, recombinant MalH, electrospray ionization mass spectrometry | Clostridium acetobutylicum |
tetramer | 4 * 51205, recombinant PagL, electrospray ionization mass spectrometry | Clostridium acetobutylicum |
tetramer | 4 * 50000, recombinant MalH and PagL, SDS-PAGE | Clostridium acetobutylicum |
Synonyms | Comment | Organism |
---|---|---|
MalH | - |
Clostridium acetobutylicum |
More | belongs to familiy 4 of the glycosylhydrolase superfamily | Clostridium acetobutylicum |
PagL | - |
Clostridium acetobutylicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Clostridium acetobutylicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Clostridium acetobutylicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NAD+-dependent | Clostridium acetobutylicum |