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2'',3''-di-O-acetyletoposide 4'-sulfate + H2O
2'',3''-di-O-acetyletoposide + sulfate
2-carboxyphenyl sulfate + H2O
2-carboxyphenol + sulfate
-
-
-
-
?
2-chloro-4-nitrophenyl sulfate + H2O
sulfate + 2-chloro-4-nitrophenol
-
-
-
-
?
2-fluoro-4-nitrophenyl sulfate + H2O
2-fluoro-4-nitrophenol + sulfate
-
-
-
?
2-hydroxy-4-nitrophenyl sulfate + H2O
2-hydroxy-4-nitrophenol + sulfate
-
-
-
-
?
2-hydroxy-5-nitrophenyl sulfate + H2O
2-hydroxy-5-nitrophenol + sulfate
2-methyl-4-nitrophenyl sulfate + H2O
2-methyl-4-nitrophenol + sulfate
-
-
-
-
?
2-nitrophenyl sulfate + H2O
2-nitrophenol + sulfate
3,4-dimethylphenyl sulfate + H2O
3,4-dimethylphenol + sulfate
-
-
-
?
3,4-dinitrophenyl sulfate + H2O
3,4-dinitrophenol + sulfate
-
-
-
?
3-bromophenyl sulfate + H2O
3-bromophenol + sulfate
-
-
-
?
3-carboxyphenyl sulfate + H2O
3-carboxyphenol + sulfate
3-chlorophenyl sulfate + H2O
3-chlorophenol + sulfate
-
-
-
?
3-cyanophenyl sulfate + H2O
3-cyanophenol + sulfate
-
-
-
?
3-fluoro-4-nitrophenyl sulfate + H2O
3-fluoro-4-nitrophenol + sulfate
-
-
-
?
3-indoxyl sulfate + H2O
1H-indol-3-ol + sulfate
-
6.4% of the activity with 4-nitrophenyl sulfate
-
-
?
3-nitrophenyl sulfate + H2O
3-nitrophenol + sulfate
4-acetylphenyl sulfate + H2O
4-acetylphenol + sulfate
4-chlorophenyl sulfate + H2O
4-chlorophenol + sulfate
-
-
-
?
4-ethylphenyl sulfate + H2O
4-ethylphenol + sulfate
-
-
-
-
?
4-fluorophenyl sulfate + H2O
4-fluorophenol + sulfate
-
-
-
?
4-hydroxy-2-nitrophenyl sulfate + H2O
4-hydroxy-2-nitrophenol + sulfate
-
-
-
-
?
4-hydroxy-3-nitrophenyl sulfate + H2O
4-hydroxy-3-nitrophenol + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferol + sulfate
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferone + sulfate
4-methylumbelliferylsulfate + H2O
4-methylumbelliferol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
6-benzoyl-2-naphthyl sulfate + H2O
6-benzoyl-2-naphthol + sulfate
-
-
-
-
?
agar + H2O
desulfated agar + sulfate
-
-
-
?
androsterone 3-sulfate + H2O
androsterone + sulfate
androsterone sulfate + H2O
androsterone + sulfate
-
-
-
-
?
apigenin-7,4'-disulfate + H2O
sulfate + ?
-
-
-
-
?
ascorbic acid 2-sulfate + H2O
ascorbic acid + sulfate
benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate methyl ester + H2O
benzyloxycarbonyl-L-Phe-L-Tyr + methylsulfate
-
-
-
-
?
boldenone 17-sulfate + H2O
boldenone + sulfate
-
-
-
?
cerebroside 3-sulfate + H2O
?
-
-
-
-
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
-
-
-
-
?
chondroitin sulfate C + H2O
chondroitin + sulfate
dehydroepiandrosterone 3-sulfate + H2O
dehydroepiandrosterone + sulfate
dopamine 3-sulfate + H2O
dopamine + sulfate
-
-
-
-
?
dopamine 4-sulfate + H2O
dopamine + sulfate
-
-
-
-
?
epiandrosterone 3-sulfate + H2O
epiandrosterone + sulfate
epinephrine 3-sulfate + H2O
epinephrine + sulfate
-
-
-
-
?
epinephrine 4-sulfate + H2O
epinephrine + sulfate
-
-
-
-
?
epitestosterone 17-sulfate + H2O
epitestosterone + sulfate
reaction of EC 3.1.6.2
-
-
?
estrone 3-sulfate + H2O
estrone + sulfate
estrone 3-sulfate + H2O
sulfate + estrone
etiocholanolone 3-sulfate + H2O
etiocholanolone + sulfate
reaction of EC 3.1.6.2
-
-
?
etoposide 4'-sulfate + H2O
etoposide + sulfate
ferulic acid 4-sulfate + H2O
ferulic acid + sulfate
-
-
-
-
?
hydroquinone sulfate + H2O
hydroquinone + sulfate
-
-
-
-
?
indoxyl sulfate + H2O
indoxol + sulfate
methylumbelliferyl sulfate + H2O
methylumbelliferone + sulfate
-
-
-
-
?
methylurolithin A sulfate + H2O
methylurolithin A + sulfate
-
-
-
-
?
N-acetyltyramine sulfate + H2O
N-acetyltyramine + sulfate
-
-
-
-
?
N-benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate + H2O
N-benzyloxycarbonyl-L-Phe-L-Tyr + -sulfate
-
-
-
-
?
nitrocatechol sulfate + H2O
nitrocatechol + sulfate
-
-
-
-
?
nordopamine 3-sulfate + H2O
nordopamine + sulfate
-
-
-
-
?
norepinephrine 3-sulfate + H2O
norepinephrine + sulfate
-
-
-
-
?
norepinephrine 4-sulfate + H2O
norepinephrine + sulfate
-
-
-
-
?
p-coumaric acid sulfate + H2O
p-coumaric acid + sulfate
-
-
-
-
?
p-cresol sulfate + H2O
p-cresol + sulfate
-
-
-
-
?
p-cresyl sulfate + H2O
p-cresol + sulfate
-
-
-
-
?
p-nitrocatechol sulfate + H2O
p-nitrocatechol + sulfate
p-nitrophenyl sulfate + H2O
p-nitrophenol + sulfate
phenyl sulfate + H2O
phenol + sulfate
phloroglucinol monosulfate + H2O
phloroglucinol + sulfate
-
-
-
-
?
resorcinol sulfate + H2O
resorcinol + sulfate
-
-
-
-
?
sulfated polysaccharide + H2O
polysaccharide + sulfate
-
-
-
?
testosterone 17-sulfate + H2O
testosterone + sulfate
reaction of EC 3.1.6.2
-
-
?
Tyr-O-sulfate + H2O
Tyr + sulfate
-
weak activity
-
-
?
tyramine-O-sulfate + H2O
tyramine + sulfate
-
weak activity
-
-
?
vanillic acid 4-sulfate + H2O
vanillic acid + sulfate
-
-
-
-
?
additional information
?
-
2'',3''-di-O-acetyletoposide 4'-sulfate + H2O

2'',3''-di-O-acetyletoposide + sulfate
-
-
-
-
?
2'',3''-di-O-acetyletoposide 4'-sulfate + H2O
2'',3''-di-O-acetyletoposide + sulfate
-
-
-
-
?
2-hydroxy-5-nitrophenyl sulfate + H2O

2-hydroxy-5-nitrophenol + sulfate
-
-
-
-
?
2-hydroxy-5-nitrophenyl sulfate + H2O
2-hydroxy-5-nitrophenol + sulfate
-
-
-
-
?
2-hydroxy-5-nitrophenyl sulfate + H2O
2-hydroxy-5-nitrophenol + sulfate
-
-
-
-
?
2-nitrophenyl sulfate + H2O

2-nitrophenol + sulfate
-
-
-
-
?
2-nitrophenyl sulfate + H2O
2-nitrophenol + sulfate
-
-
-
-
?
2-nitrophenyl sulfate + H2O
2-nitrophenol + sulfate
-
-
-
-
?
2-nitrophenyl sulfate + H2O
2-nitrophenol + sulfate
-
-
-
-
?
3-carboxyphenyl sulfate + H2O

3-carboxyphenol + sulfate
-
weak activity
-
-
?
3-carboxyphenyl sulfate + H2O
3-carboxyphenol + sulfate
-
weak activity
-
-
?
3-nitrophenyl sulfate + H2O

3-nitrophenol + sulfate
-
-
-
-
?
3-nitrophenyl sulfate + H2O
3-nitrophenol + sulfate
-
-
-
-
?
3-nitrophenyl sulfate + H2O
3-nitrophenol + sulfate
-
-
-
?
4-acetylphenyl sulfate + H2O

4-acetylphenol + sulfate
-
-
-
-
?
4-acetylphenyl sulfate + H2O
4-acetylphenol + sulfate
-
-
-
-
?
4-acetylphenyl sulfate + H2O
4-acetylphenol + sulfate
-
-
-
-
?
4-acetylphenyl sulfate + H2O
4-acetylphenol + sulfate
-
-
-
-
?
4-acetylphenyl sulfate + H2O
4-acetylphenol + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O

4-methylumbelliferol + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferol + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferol + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferol + sulfate
-
rather poor substrate
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferol + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferol + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O

4-methylumbelliferone + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferone + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferone + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferone + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferone + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferone + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferone + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O

4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
77% of the activity with 4-nitrophenyl sulfate
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
best substrate
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrophenyl phosphate + H2O

4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
the enzyme is able to catalyze the hydrolysis of the original 4-nitrophenyl sulfate substrate and the promiscuous 4-nitrophenyl phosphate. Only some steps of the promiscuous reaction are identical to those in the native process. Differences concern mainly the last step in which the His115 residue acts as a general base to accept the proton by the O atom of the FGly51 in the 4-nitrophenyl sulfate, whereas in 4-nitrophenyl phosphate, the Asp317 protonated residue works as a general acid to deliver a proton by a water molecule to the oxygen atom of the C-O bond. The rate-determining steps are different in the two reactions, i.e. nucleophile attack vs. nucleophile activation
-
-
?
4-nitrophenyl sulfate + H2O

4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
the enzyme is able to catalyze the hydrolysis of the original 4-nitrophenyl sulfate substrate and the promiscuous 4-nitrophenyl phosphate. Only some steps of the promiscuous reaction are identical to those in the native process. Differences concern mainly the last step in which the His115 residue acts as a general base to accept the proton by the O atom of the FGly51 in the 4-nitrophenyl sulfate, whereas in 4-nitrophenyl phosphate, the Asp317 protonated residue works as a general acid to deliver a proton by a water molecule to the oxygen atom of the C-O bond. The rate-determining steps are different in the two reactions, i.e. nucleophile attack vs. nucleophile activation
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate