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2'',3''-di-O-acetyletoposide 4'-sulfate + H2O
2'',3''-di-O-acetyletoposide + sulfate
2-carboxyphenyl sulfate + H2O
2-carboxyphenol + sulfate
-
-
-
-
?
2-chloro-4-nitrophenyl sulfate + H2O
sulfate + 2-chloro-4-nitrophenol
-
-
-
-
?
2-fluoro-4-nitrophenyl sulfate + H2O
2-fluoro-4-nitrophenol + sulfate
-
-
-
?
2-hydroxy-4-nitrophenyl sulfate + H2O
2-hydroxy-4-nitrophenol + sulfate
-
-
-
-
?
2-hydroxy-5-nitrophenyl sulfate + H2O
2-hydroxy-5-nitrophenol + sulfate
2-methyl-4-nitrophenyl sulfate + H2O
2-methyl-4-nitrophenol + sulfate
-
-
-
-
?
2-nitrophenyl sulfate + H2O
2-nitrophenol + sulfate
3,4-dimethylphenyl sulfate + H2O
3,4-dimethylphenol + sulfate
-
-
-
?
3,4-dinitrophenyl sulfate + H2O
3,4-dinitrophenol + sulfate
-
-
-
?
3-bromophenyl sulfate + H2O
3-bromophenol + sulfate
-
-
-
?
3-carboxyphenyl sulfate + H2O
3-carboxyphenol + sulfate
3-chlorophenyl sulfate + H2O
3-chlorophenol + sulfate
-
-
-
?
3-cyanophenyl sulfate + H2O
3-cyanophenol + sulfate
-
-
-
?
3-fluoro-4-nitrophenyl sulfate + H2O
3-fluoro-4-nitrophenol + sulfate
-
-
-
?
3-indoxyl sulfate + H2O
1H-indol-3-ol + sulfate
-
6.4% of the activity with 4-nitrophenyl sulfate
-
-
?
3-nitrophenyl sulfate + H2O
3-nitrophenol + sulfate
4-acetylphenyl sulfate + H2O
4-acetylphenol + sulfate
4-chlorophenyl sulfate + H2O
4-chlorophenol + sulfate
-
-
-
?
4-ethylphenyl sulfate + H2O
4-ethylphenol + sulfate
-
-
-
-
?
4-fluorophenyl sulfate + H2O
4-fluorophenol + sulfate
-
-
-
?
4-hydroxy-2-nitrophenyl sulfate + H2O
4-hydroxy-2-nitrophenol + sulfate
-
-
-
-
?
4-hydroxy-3-nitrophenyl sulfate + H2O
4-hydroxy-3-nitrophenol + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferol + sulfate
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferone + sulfate
4-methylumbelliferylsulfate + H2O
4-methylumbelliferol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
6-benzoyl-2-naphthyl sulfate + H2O
6-benzoyl-2-naphthol + sulfate
-
-
-
-
?
agar + H2O
desulfated agar + sulfate
-
-
-
?
androsterone 3-sulfate + H2O
androsterone + sulfate
androsterone sulfate + H2O
androsterone + sulfate
-
-
-
-
?
apigenin-7,4'-disulfate + H2O
sulfate + ?
-
-
-
-
?
ascorbic acid 2-sulfate + H2O
ascorbic acid + sulfate
benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate methyl ester + H2O
benzyloxycarbonyl-L-Phe-L-Tyr + methylsulfate
-
-
-
-
?
boldenone 17-sulfate + H2O
boldenone + sulfate
-
-
-
?
cerebroside 3-sulfate + H2O
?
-
-
-
-
?
cerebroside 3-sulfate + H2O
cerebroside + sulfate
-
-
-
-
?
chondroitin sulfate C + H2O
chondroitin + sulfate
dehydroepiandrosterone 3-sulfate + H2O
dehydroepiandrosterone + sulfate
dopamine 3-sulfate + H2O
dopamine + sulfate
-
-
-
-
?
dopamine 4-sulfate + H2O
dopamine + sulfate
-
-
-
-
?
epiandrosterone 3-sulfate + H2O
epiandrosterone + sulfate
epinephrine 3-sulfate + H2O
epinephrine + sulfate
-
-
-
-
?
epinephrine 4-sulfate + H2O
epinephrine + sulfate
-
-
-
-
?
epitestosterone 17-sulfate + H2O
epitestosterone + sulfate
reaction of EC 3.1.6.2
-
-
?
estrone 3-sulfate + H2O
estrone + sulfate
estrone 3-sulfate + H2O
sulfate + estrone
etiocholanolone 3-sulfate + H2O
etiocholanolone + sulfate
reaction of EC 3.1.6.2
-
-
?
etoposide 4'-sulfate + H2O
etoposide + sulfate
ferulic acid 4-sulfate + H2O
ferulic acid + sulfate
-
-
-
-
?
hydroquinone sulfate + H2O
hydroquinone + sulfate
-
-
-
-
?
indoxyl sulfate + H2O
indoxol + sulfate
methylumbelliferyl sulfate + H2O
methylumbelliferone + sulfate
-
-
-
-
?
methylurolithin A sulfate + H2O
methylurolithin A + sulfate
-
-
-
-
?
N-acetyltyramine sulfate + H2O
N-acetyltyramine + sulfate
-
-
-
-
?
N-benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate + H2O
N-benzyloxycarbonyl-L-Phe-L-Tyr + -sulfate
-
-
-
-
?
nitrocatechol sulfate + H2O
nitrocatechol + sulfate
-
-
-
-
?
nordopamine 3-sulfate + H2O
nordopamine + sulfate
-
-
-
-
?
norepinephrine 3-sulfate + H2O
norepinephrine + sulfate
-
-
-
-
?
norepinephrine 4-sulfate + H2O
norepinephrine + sulfate
-
-
-
-
?
p-coumaric acid sulfate + H2O
p-coumaric acid + sulfate
-
-
-
-
?
p-cresol sulfate + H2O
p-cresol + sulfate
-
-
-
-
?
p-cresyl sulfate + H2O
p-cresol + sulfate
-
-
-
-
?
p-nitrocatechol sulfate + H2O
p-nitrocatechol + sulfate
p-nitrophenyl sulfate + H2O
p-nitrophenol + sulfate
phenyl sulfate + H2O
phenol + sulfate
phloroglucinol monosulfate + H2O
phloroglucinol + sulfate
-
-
-
-
?
resorcinol sulfate + H2O
resorcinol + sulfate
-
-
-
-
?
sulfated polysaccharide + H2O
polysaccharide + sulfate
-
-
-
?
testosterone 17-sulfate + H2O
testosterone + sulfate
reaction of EC 3.1.6.2
-
-
?
Tyr-O-sulfate + H2O
Tyr + sulfate
-
weak activity
-
-
?
tyramine-O-sulfate + H2O
tyramine + sulfate
-
weak activity
-
-
?
vanillic acid 4-sulfate + H2O
vanillic acid + sulfate
-
-
-
-
?
additional information
?
-
2'',3''-di-O-acetyletoposide 4'-sulfate + H2O

2'',3''-di-O-acetyletoposide + sulfate
-
-
-
-
?
2'',3''-di-O-acetyletoposide 4'-sulfate + H2O
2'',3''-di-O-acetyletoposide + sulfate
-
-
-
-
?
2-hydroxy-5-nitrophenyl sulfate + H2O

2-hydroxy-5-nitrophenol + sulfate
-
-
-
-
?
2-hydroxy-5-nitrophenyl sulfate + H2O
2-hydroxy-5-nitrophenol + sulfate
-
-
-
-
?
2-hydroxy-5-nitrophenyl sulfate + H2O
2-hydroxy-5-nitrophenol + sulfate
-
-
-
-
?
2-nitrophenyl sulfate + H2O

2-nitrophenol + sulfate
-
-
-
-
?
2-nitrophenyl sulfate + H2O
2-nitrophenol + sulfate
-
-
-
-
?
2-nitrophenyl sulfate + H2O
2-nitrophenol + sulfate
-
-
-
-
?
2-nitrophenyl sulfate + H2O
2-nitrophenol + sulfate
-
-
-
-
?
3-carboxyphenyl sulfate + H2O

3-carboxyphenol + sulfate
-
weak activity
-
-
?
3-carboxyphenyl sulfate + H2O
3-carboxyphenol + sulfate
-
weak activity
-
-
?
3-nitrophenyl sulfate + H2O

3-nitrophenol + sulfate
-
-
-
-
?
3-nitrophenyl sulfate + H2O
3-nitrophenol + sulfate
-
-
-
-
?
3-nitrophenyl sulfate + H2O
3-nitrophenol + sulfate
-
-
-
?
4-acetylphenyl sulfate + H2O

4-acetylphenol + sulfate
-
-
-
-
?
4-acetylphenyl sulfate + H2O
4-acetylphenol + sulfate
-
-
-
-
?
4-acetylphenyl sulfate + H2O
4-acetylphenol + sulfate
-
-
-
-
?
4-acetylphenyl sulfate + H2O
4-acetylphenol + sulfate
-
-
-
-
?
4-acetylphenyl sulfate + H2O
4-acetylphenol + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O

4-methylumbelliferol + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferol + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferol + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferol + sulfate
-
rather poor substrate
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferol + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferol + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O

4-methylumbelliferone + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferone + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferone + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferone + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferone + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferone + sulfate
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferone + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O

4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
77% of the activity with 4-nitrophenyl sulfate
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
best substrate
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrocatechol sulfate + H2O
4-nitrocatechol + sulfate
-
-
-
-
?
4-nitrophenyl phosphate + H2O

4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
the enzyme is able to catalyze the hydrolysis of the original 4-nitrophenyl sulfate substrate and the promiscuous 4-nitrophenyl phosphate. Only some steps of the promiscuous reaction are identical to those in the native process. Differences concern mainly the last step in which the His115 residue acts as a general base to accept the proton by the O atom of the FGly51 in the 4-nitrophenyl sulfate, whereas in 4-nitrophenyl phosphate, the Asp317 protonated residue works as a general acid to deliver a proton by a water molecule to the oxygen atom of the C-O bond. The rate-determining steps are different in the two reactions, i.e. nucleophile attack vs. nucleophile activation
-
-
?
4-nitrophenyl sulfate + H2O

4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
the enzyme is able to catalyze the hydrolysis of the original 4-nitrophenyl sulfate substrate and the promiscuous 4-nitrophenyl phosphate. Only some steps of the promiscuous reaction are identical to those in the native process. Differences concern mainly the last step in which the His115 residue acts as a general base to accept the proton by the O atom of the FGly51 in the 4-nitrophenyl sulfate, whereas in 4-nitrophenyl phosphate, the Asp317 protonated residue works as a general acid to deliver a proton by a water molecule to the oxygen atom of the C-O bond. The rate-determining steps are different in the two reactions, i.e. nucleophile attack vs. nucleophile activation
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
the 4-nitrocatechol sulfate substrate is hydrolyzed to a greater extent than the 4-nitrophenyl sulfate substrate
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
4-nitrophenol + sulfate
-
-
-
?
agar + H2O

?
-
-
-
-
?
agar + H2O
?
sulfate content of agar is diminished to 40% after a 12 h treatment
-
-
?
agar + H2O
?
sulfate content of agar is diminished to 40% after a 12 h treatment
-
-
?
androsterone 3-sulfate + H2O

androsterone + sulfate
reaction of EC 3.1.6.2
-
-
?
androsterone 3-sulfate + H2O
androsterone + sulfate
reaction of EC 3.1.6.2
-
-
?
ascorbic acid 2-sulfate + H2O

ascorbic acid + sulfate
-
-
-
-
?
ascorbic acid 2-sulfate + H2O
ascorbic acid + sulfate
-
-
-
-
?
ascorbic acid 2-sulfate + H2O
ascorbic acid + sulfate
-
-
-
-
?
chondroitin sulfate C + H2O

chondroitin + sulfate
-
-
-
-
?
chondroitin sulfate C + H2O
chondroitin + sulfate
-
-
-
-
?
chondroitin sulfate C + H2O
chondroitin + sulfate
-
-
-
-
?
dehydroepiandrosterone 3-sulfate + H2O

dehydroepiandrosterone + sulfate
reaction of EC 3.1.6.2
-
-
?
dehydroepiandrosterone 3-sulfate + H2O
dehydroepiandrosterone + sulfate
reaction of EC 3.1.6.2
-
-
?
epiandrosterone 3-sulfate + H2O

epiandrosterone + sulfate
reaction of EC 3.1.6.2
-
-
?
epiandrosterone 3-sulfate + H2O
epiandrosterone + sulfate
reaction of EC 3.1.6.2
-
-
?
estrone 3-sulfate + H2O

estrone + sulfate
-
-
-
-
?
estrone 3-sulfate + H2O
estrone + sulfate
reaction of EC 3.1.6.2
-
-
?
estrone 3-sulfate + H2O
estrone + sulfate
reaction of EC 3.1.6.2
-
-
?
estrone 3-sulfate + H2O

sulfate + estrone
-
-
-
-
?
estrone 3-sulfate + H2O
sulfate + estrone
-
-
-
-
?
estrone 3-sulfate + H2O
sulfate + estrone
-
-
-
-
?
etoposide 4'-sulfate + H2O

etoposide + sulfate
-
-
-
-
?
etoposide 4'-sulfate + H2O
etoposide + sulfate
-
-
-
-
?
indoxyl sulfate + H2O

indoxol + sulfate
-
-
-
-
?
indoxyl sulfate + H2O
indoxol + sulfate
-
weak activity
-
-
?
p-nitrocatechol sulfate + H2O

p-nitrocatechol + sulfate
-
-
-
-
?
p-nitrocatechol sulfate + H2O
p-nitrocatechol + sulfate
-
-
-
-
?
p-nitrocatechol sulfate + H2O
p-nitrocatechol + sulfate
-
-
-
-
?
p-nitrocatechol sulfate + H2O
p-nitrocatechol + sulfate
-
-
-
-
?
p-nitrophenyl sulfate + H2O

p-nitrophenol + sulfate
-
-
-
-
?
p-nitrophenyl sulfate + H2O
p-nitrophenol + sulfate
-
-
-
-
?
p-nitrophenyl sulfate + H2O
p-nitrophenol + sulfate
Littorina kurila
-
-
-
-
?
p-nitrophenyl sulfate + H2O
p-nitrophenol + sulfate
-
-
-
-
?
p-nitrophenyl sulfate + H2O
p-nitrophenol + sulfate
-
-
-
-
?
p-nitrophenyl sulfate + H2O
p-nitrophenol + sulfate
-
-
-
-
?
phenyl sulfate + H2O

phenol + sulfate
-
weak activity
-
-
?
phenyl sulfate + H2O
phenol + sulfate
-
-
-
-
?
additional information

?
-
-
no substrate: 5-bromo-4-chloro-3-indolyl sulfate, phenolphthalein disulfate, galactose 6-sulfate
-
-
?
additional information
?
-
-
enzyme might be of physiological significance in the deconjugation of the catecholamine sulfate isomers
-
-
?
additional information
?
-
-
ASG does not undergo lysosomal processing
-
-
?
additional information
?
-
activity is dependent on residue cysteine80, which undergoes conversion to formylglycine. No substrate: 4-methylumbelliferyl sulfate
-
-
?
additional information
?
-
-
activity is dependent on residue cysteine80, which undergoes conversion to formylglycine. No substrate: 4-methylumbelliferyl sulfate
-
-
?
additional information
?
-
-
enzyme is able to disperse the cumulus matrix of expanded cumulus oocyte complexes, this dispersion action is not associated with desulfation activity
-
-
?
additional information
?
-
-
AtsBCA is controlled by CysB protein (a LysR-type transcriptional activator) in Pseudomonas aeruginosa
-
-
?
additional information
?
-
the mechanism for S-O-bridge bond fission shifts, with decreasing leaving group ability, from charge compensation via Lewis acid interactions toward direct proton donation. The transition state for enzyme-catalyzed sulfate monoester hydrolysis has a significantly more associative character compared to the uncatalyzed reaction
-
-
?
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1,2,3-benzoxathiazine 2,2-dioxide
-
irreversible
2-(difluoromethyl)phenyl hydrogen sulfate
-
competitive
2-hydroxy-4-methyl-5-nitrophenyl sulfate
-
substrate inhibition
3,4-dihydro-1,2,3-benzoxathiazine 2,2-dioxide
-
irreversible
3-(alpha-acetonylbenzyl)-4-hydroxycoumarin
-
2.5 mM results in 50% reduced activity of arylsulfatase
3H-1,2,3-benzoxathiazole 2,2-dioxide
-
irreversible
4-(difluoromethyl)phenyl hydrogen sulfate
-
competitive
5-sulfosalicylic acid
-
strong
AgNO3
-
0.2 mM, 20-30% inhibition
Al3+
1 mM, less than 20% residual activity, both free and immobilized enzyme
Fe2+
1 mM, less than 20% residual activity, both free and immobilized enzyme
guanidine hydrochloride
1 mM, 69.5% of initial activity, mutant H260L
iodoacetic acid
-
10 mM, 48% residual activity
IPTG
-
at concentrations from 50 micromolar up to 5000 micromolar of IPTG the activity decreases by more than 50%
KCN
-
10 mM, 23% residual activity
Mg2+
1 mM, 88% of initial activity, mutant H260L
Mn2+
10 mM, 69% residual activity
N-benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate methyl ester
-
hydrolysis of 4-nitrophenyl sulfate
Na2B4O7
-
10 mM, 12% residual activity
p-(amidinophenyl)methanesulfonyl fluoride
-
-
p-(amidinophenyl)methanesulfonyl fluoride HCl
-
-
Phenolphthalein disulfate
-
hydrolysis of 4-nitrophenyl sulfate
phenyl sulfamate
-
irreversible
praziquantel
-
schistosomical drug, inhibitory to enzyme. No effect on immunological properties of enzyme
sodium dodecyl sulfonate
-
-
sodium dodecylsulfate
-
1 mM, 4% residual activity
sodium phenylmethane sulfonate
-
-
Triton X-100
1 mM, 15% residual activity, free enzyme, 35% residual activity, immobilized enzyme, respectively
Tween 80
1 mM, 10% residual activity, free enzyme, 60% residual activity, immobilized enzyme, respectively
ZnCl2
-
10 mM, 10% residual activity
(NH4)2SO4

-
-
2-mercaptoethanol

10 mM, 43% residual activity
2-mercaptoethanol
1 mM, 70% residual activity, both free and immobilized enzyme
2-mercaptoethanol
1 mM, 81% of initial activity, mutant H260L
4-nitrophenyl phosphate

-
hydrolysis of 4-nitrophenyl sulfate
4-nitrophenyl phosphate
-
-
4-nitrophenyl sulfate

-
substrate inhibition above 12 mM
4-nitrophenyl sulfate
-
substrate inhibition
Ag+

-
-
Ag+
1 mM, less than 20% residual activity, both free and immobilized enzyme
AlCl3

-
-
BaCl2

-
-
Borate

-
-
Cd2+

1 mM, less than 20% residual activity, both free and immobilized enzyme
Cd2+
1 mM, 65% of initial activity, mutant H260L
CdCl2

-
-
citrate

-
-
CN-

-
weak inhibitor
CoCl2

-
-
Cu2+

10 mM, 67% residual activity
Cu2+
1 mM, 16% residual activity
Cu2+
1 mM, less than 20% residual activity, both free and immobilized enzyme
Cu2+
1 mM, 9% of initial activity, mutant H260L
CuCl2

-
-
Cys

-
-
dithiothreitol

10 mM, 55% residual activity
dithiothreitol
1 mM, 69.8% of initial activity, mutant H260L
dopamine

-
-
EDTA

10 mM, 33% residual activity
EDTA
-
0.1 mM EDTA 46.8% relative activity, 0.5 mM EDTA 39.6% relative activity and 1.0 mM EDTA 30.5% relative activity compared to the control
EDTA
1 mM, 125.6% of initial activity, mutant H260L
F-

-
NaF
Fe3+

1 mM, less than 20% residual activity, both free and immobilized enzyme
FeCl2

-
-
FeSO4

-
-
Hg2+

-
HgCl2
hydroxylamine

-
-
iodoacetate

-
-
K2HPO4

-
-
K2HPO4
-
5 mM, 97-98% inhibition
K2SO3

-
-
KCl

-
-
MnCl2

-
-
Na2SO3

-
-
Na3PO4

-
competitive
NaCl

-
125 mM, 20-30% inhibition
NEM

-
-
NiCl2

-
-
octopamine

-
-
PCMB

-
-
phosphate

-
acts as a strong, competitive ASG inhibitor, inhibition of ASG by phosphate is much stronger than by sulfate
PMSF

-
0.1 mM PMSF 92.7% relative activity, 0.5 mM PMSF 82.6% relative activity and 1.0 mM PMSF 73.5% relative activity compared to the control
PMSF
1 mM, 15% residual activity, free enzyme, 35% residual activity, immobilized enzyme, respectively
PO43-

-
potent inhibitor
SDS

1 mM, 5% residual activity, free enzyme, 45% residual activity, immobilized enzyme, respectively
SO32-

-
potent inhibitor
SO42-

-
FeSO4
SO42-
-
competitive; Na2SO4
SO42-
-
12.5 mM K2SO4, 80-85% inhibition
sulfate

-
-
tyramine

-
10 mM, 14% residual activity
Urea

10 mM, 66% residual activity
Urea
1 mM, 86% of initial activity, mutant H260L
Zn2+

-
ZnSO4
Zn2+
10 mM, 78% residual activity
Zn2+
1 mM, 43% residual activity
Zn2+
1 mM, less than 20% residual activity, both free and immobilized enzyme
Zn2+
1 mM, complete loss of activity, mutant H260L
additional information

-
SO42- and Ag+ ions have no effect on the enzyme activity
-
additional information
-
ASG is not inhibited by warfarin even at concentrations close to the solubility limit
-
additional information
neither inhibitory nor activating: Ca2+, K+, Ba2+, Mg2+, Mn2+ and Sn2+
-
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0.181
2-hydroxy-4-nitrophenyl sulfate
-
in presence of 100 mM acetate
0.043 - 0.085
2-hydroxy-5-nitrophenyl sulfate
0.55
2-methyl-4-nitrophenyl sulfate
-
pH 8.9, 30ưC
1.02
4-ethylphenyl sulfate
-
pH 7.0, temperature not specified in the publication
0.123
4-hydroxy-2-nitrophenyl sulfate
-
in presence of 100 mM acetate
0.193
4-hydroxy-3-nitrophenyl sulfate
-
in presence of 100 mM acetate
2.5
4-methylumbelliferone sulfate
-
-
0.0066 - 0.175
4-Methylumbelliferyl sulfate
0.105 - 20.6
4-nitrocatechol sulfate
0.0291 - 0.0752
4-nitrophenyl phosphate
0.00025 - 26
4-nitrophenyl sulfate
0.5
Ascorbate 2-sulfate
-
-
2.5
Ascorbic acid 2-sulfate
-
-
0.13
cerebroside 3-sulfate
-
-
7.2 - 9.8
dopamine 3-sulfate
2.1 - 2.8
dopamine 4-sulfate
0.026
epiandrosterone 3-sulfate
pH 8.8, 37ưC
14.1 - 18
epinephrine 3-sulfate
1.9 - 2.5
epinephrine 4-sulfate
0.014 - 0.351
Estrone 3-sulfate
0.161
ferulic acid 4-sulfate
-
pH 7.0, temperature not specified in the publication
0.216
Indoxyl sulfate
-
pH 7.0, temperature not specified in the publication
0.112
methylurolithin A sulfate
-
pH 7.0, temperature not specified in the publication
-
1.486
N-acetyltyramine sulfate
-
pH 7.0, temperature not specified in the publication
1.05
N-benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate
-
-
10.5 - 12.8
norepinephrine 3-sulfate
2.7 - 3.3
norepinephrine 4-sulfate
0.262
p-coumaric acid sulfate
-
pH 7.0, temperature not specified in the publication
1.4
p-cresyl sulfate
-
pH 7.0, temperature not specified in the publication
0.0549 - 8.7
p-Nitrophenyl sulfate
3.65
phloroglucinol monosulfate
-
-
0.131
resorcinol sulfate
-
pH 7.0, temperature not specified in the publication
0.05
testosterone 17-sulfate
pH 8.8, 37ưC
0.341
vanillic acid 4-sulfate
-
pH 7.0, temperature not specified in the publication
0.043
2-hydroxy-5-nitrophenyl sulfate

-
in presence of 400 mM acetate
0.0486
2-hydroxy-5-nitrophenyl sulfate
-
in presence of 25-400 mM acetate
0.085
2-hydroxy-5-nitrophenyl sulfate
-
in presence of 100 mM acetate and 100 mM sulfate
0.0066
4-Methylumbelliferyl sulfate

-
pH 8.9, 30ưC
0.175
4-Methylumbelliferyl sulfate
-
pH 7.0, temperature not specified in the publication
0.105
4-nitrocatechol sulfate

-
-
0.16
4-nitrocatechol sulfate
-
-
0.166
4-nitrocatechol sulfate
-
beta enzyme
0.2
4-nitrocatechol sulfate
-
enzyme from fraction II and fraction III
0.204
4-nitrocatechol sulfate
-
alpha enzyme
0.21
4-nitrocatechol sulfate
-
pH 5.0, 37ưC, wild-type
0.23
4-nitrocatechol sulfate
-
enzyme from fraction I
0.25
4-nitrocatechol sulfate
-
0.25 M acetate buffer, pH 5.0
0.26
4-nitrocatechol sulfate
-
pH 5.0, 37ưC, patient with cerebral palsy
0.3
4-nitrocatechol sulfate
-
-
0.5
4-nitrocatechol sulfate
-
0.25 M acetate buffer, pH 5.6
0.68
4-nitrocatechol sulfate
-
arylsulfatase B
1.1
4-nitrocatechol sulfate
-
-
1.8
4-nitrocatechol sulfate
-
arylsulfatase A
2.5
4-nitrocatechol sulfate
-
-
2.9
4-nitrocatechol sulfate
-
arylsulfatase B
3.2
4-nitrocatechol sulfate
-
arylsulfatase A
6
4-nitrocatechol sulfate
-
pH 7.5, 45ưC
10.9
4-nitrocatechol sulfate
pH 4.6, 37ưC
20.6
4-nitrocatechol sulfate
pH 4.6, 37ưC
0.0291
4-nitrophenyl phosphate

-
wild type enzyme, in 100 mM Tris, pH 8.0, 25ưC
0.0752
4-nitrophenyl phosphate
-
mutant enzyme C51S, in 100 mM Tris, pH 8.0, 25ưC
0.00025
4-nitrophenyl sulfate

-
mutant enzyme C51S, in 100 mM Tris, pH 8.0, 25ưC
0.00029
4-nitrophenyl sulfate
-
wild type enzyme, in 100 mM Tris, pH 8.0, 25ưC
0.0004
4-nitrophenyl sulfate
-
mutant enzyme C51A, in 100 mM Tris, pH 8.0, 25ưC
0.0037
4-nitrophenyl sulfate
-
in presence of 1 mM CaCl2
0.007
4-nitrophenyl sulfate
pH 8.8, 37ưC
0.0137
4-nitrophenyl sulfate
pH not specified in the publication, temperature not specified in the publication
0.017
4-nitrophenyl sulfate
-
in absence CaCl2
0.0294
4-nitrophenyl sulfate
-
alpha enzyme
0.042
4-nitrophenyl sulfate
-
pH 8.9, 30ưC
0.07
4-nitrophenyl sulfate
-
without imidazole
0.075
4-nitrophenyl sulfate
-
beta enzyme
0.08
4-nitrophenyl sulfate
-
-
0.2
4-nitrophenyl sulfate
-
with imidazole
0.27
4-nitrophenyl sulfate
-
-
0.3
4-nitrophenyl sulfate
-
-
0.53
4-nitrophenyl sulfate
-
-
0.59
4-nitrophenyl sulfate
-
-
0.66
4-nitrophenyl sulfate
mutant H260L, pH 7.5, 55ưC
0.71
4-nitrophenyl sulfate
wild-type, pH 7.5, 55ưC
0.82
4-nitrophenyl sulfate
-
pH 7.4, 29.85ưC, pressure: 160 MPa
0.89
4-nitrophenyl sulfate
pH 7.5, 50ưC, immobilized enzyme
1.12
4-nitrophenyl sulfate
-
pH 7.4, 29.85ưC, pressure: 120 MPa
1.2
4-nitrophenyl sulfate
-
pH 7.08, 30ưC
1.5 - 2
4-nitrophenyl sulfate
-
pH 7.4, 24.85ưC, pressure: 0.1 MPa
1.6
4-nitrophenyl sulfate
-
pH 7.4, 29.85ưC, pressure: 5 MPa
1.61
4-nitrophenyl sulfate
-
pH 7.4, 29.85ưC, pressure: 40 MPa
1.62
4-nitrophenyl sulfate
-
pH 7.4, 29.85ưC, pressure: 80 MPa
1.85
4-nitrophenyl sulfate
-
pH 7.43, 30ưC
2.01
4-nitrophenyl sulfate
-
pH 7.4, 19.85ưC, pressure: 0.1 MPa
2.12
4-nitrophenyl sulfate
-
pH 7.4, 29.85ưC, pressure: 0.1 MPa
2.31
4-nitrophenyl sulfate
-
pH 7.4, 22.35ưC, pressure: 0.1 MPa
2.47
4-nitrophenyl sulfate
-
pH 7.4, 14.85ưC, pressure: 0.1 MPa
2.49
4-nitrophenyl sulfate
-
pH 7.4, 17.35ưC, pressure: 0.1 MPa
2.5
4-nitrophenyl sulfate
-
pH 7.4, 34.85ưC, pressure: 0.1 MPa
4.36
4-nitrophenyl sulfate
-
pH 8.08, 30ưC
5.26
4-nitrophenyl sulfate
-
pH 8.68, 30ưC
26
4-nitrophenyl sulfate
-
-
7.2
dopamine 3-sulfate

-
pH 6.9
9.8
dopamine 3-sulfate
-
pH 7.4
2.1
dopamine 4-sulfate

-
pH 6.9
2.8
dopamine 4-sulfate
-
pH 7.4
14.1
epinephrine 3-sulfate

-
pH 6.9
18
epinephrine 3-sulfate
-
pH 7.4
1.9
epinephrine 4-sulfate

-
pH 6.9
2.5
epinephrine 4-sulfate
-
pH 7.4
0.014
Estrone 3-sulfate

pH 8.8, 37ưC
0.344
Estrone 3-sulfate
-
-
0.351
Estrone 3-sulfate
-
pH 7.0, temperature not specified in the publication
10.5
norepinephrine 3-sulfate

-
pH 6.9
12.8
norepinephrine 3-sulfate
-
pH 7.4
2.7
norepinephrine 4-sulfate

-
pH 6.9
3.3
norepinephrine 4-sulfate
-
pH 7.4
0.0549
p-Nitrophenyl sulfate

-
-
0.67
p-Nitrophenyl sulfate
-
6.5 nM purified arylsulfatase incubated at 45ưC and at pH 7.0 in the presence of 10 mM sulfate
1.15
p-Nitrophenyl sulfate
-
6.5 nM purified arylsulfatase incubated at 45ưC and at pH 7.0
8.7
p-Nitrophenyl sulfate
Littorina kurila
-
37ưC, pH 5.4
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7150
4-ethylphenyl sulfate
-
pH 7.0, temperature not specified in the publication
28600
4-Methylumbelliferyl sulfate
-
pH 7.0, temperature not specified in the publication
0.0000095 - 0.023
4-nitrophenyl phosphate
0.000061 - 256.9
4-nitrophenyl sulfate
40900
Estrone 3-sulfate
-
pH 7.0, temperature not specified in the publication
138
ferulic acid 4-sulfate
-
pH 7.0, temperature not specified in the publication
228
Indoxyl sulfate
-
pH 7.0, temperature not specified in the publication
88500
methylurolithin A sulfate
-
pH 7.0, temperature not specified in the publication
-
1800
N-acetyltyramine sulfate
-
pH 7.0, temperature not specified in the publication
235
p-coumaric acid sulfate
-
pH 7.0, temperature not specified in the publication
871
p-cresyl sulfate
-
pH 7.0, temperature not specified in the publication
1000
p-Nitrophenyl sulfate
-
6.5 nM purified arylsulfatase, at 45ưC and pH 7.0
92
resorcinol sulfate
-
pH 7.0, temperature not specified in the publication
119
vanillic acid 4-sulfate
-
pH 7.0, temperature not specified in the publication
0.0000095
4-nitrophenyl phosphate

-
mutant enzyme C51S, in 100 mM Tris, pH 8.0, 25ưC
0.023
4-nitrophenyl phosphate
-
wild type enzyme, in 100 mM Tris, pH 8.0, 25ưC
0.000061
4-nitrophenyl sulfate

-
mutant enzyme C51A, in 100 mM Tris, pH 8.0, 25ưC
0.0054
4-nitrophenyl sulfate
-
mutant enzyme C51S, in 100 mM Tris, pH 8.0, 25ưC
14.2
4-nitrophenyl sulfate
-
wild type enzyme, in 100 mM Tris, pH 8.0, 25ưC
40.5
4-nitrophenyl sulfate
-
pH 7.4, 29.85ưC, pressure: 120 MPa
41.83
4-nitrophenyl sulfate
-
pH 7.4, 17.35ưC, pressure: 0.1 MPa
42.5
4-nitrophenyl sulfate
-
pH 7.4, 29.85ưC, pressure: 160 MPa
42.83
4-nitrophenyl sulfate
-
pH 7.4, 14.85ưC, pressure: 0.1 MPa
46.16
4-nitrophenyl sulfate
-
pH 7.4, 19.85ưC, pressure: 0.1 MPa
46.5
4-nitrophenyl sulfate
-
pH 7.4, 29.85ưC, pressure: 40 MPa
48.33
4-nitrophenyl sulfate
-
pH 7.4, 29.85ưC, pressure: 5 MPa
51.33
4-nitrophenyl sulfate
-
pH 7.4, 29.85ưC, pressure: 80 MPa
56.33
4-nitrophenyl sulfate
-
pH 7.4, 24.85ưC, pressure: 0.1 MPa
58
4-nitrophenyl sulfate
-
pH 7.4, 22.35ưC, pressure: 0.1 MPa
74.5
4-nitrophenyl sulfate
-
pH 7.4, 29.85ưC, pressure: 0.1 MPa
90.83
4-nitrophenyl sulfate
-
pH 7.4, 34.85ưC, pressure: 0.1 MPa
256.9
4-nitrophenyl sulfate
pH 7.5, 50ưC, immobilized enzyme
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