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Literature summary for 3.1.6.1 extracted from
- Characterization and partial purification of arylsulfatase from the seminal plasma of the sea urchin, Strongylocentrotus intermedius (1980), Arch. Biochem. Biophys., 201, 216-223.
General Stability
| General Stability | Organism |
|---|---|
| several cycles of freezing and thawing cause a marked reduction of activity | Strongylocentrotus intermedius |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Strongylocentrotus intermedius |  |
| EDTA | - |
Strongylocentrotus intermedius | |
| Fe3+ | - |
Strongylocentrotus intermedius | |
| iodoacetate | - |
Strongylocentrotus intermedius | |
| Zn2+ | - |
Strongylocentrotus intermedius | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
Ascorbate 2-sulfate | - |
Strongylocentrotus intermedius | |
| 0.53 | - |
4-nitrophenyl sulfate | - |
Strongylocentrotus intermedius | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | can partially replace Ca2+ | Strongylocentrotus intermedius | |
| Ca2+ | required | Strongylocentrotus intermedius | |
| KCl | stimulates | Strongylocentrotus intermedius | |
| Mg2+ | can partially replace Ca2+ | Strongylocentrotus intermedius | |
| NaCl | stimulates | Strongylocentrotus intermedius | |
| Sr2+ | can partially replace Ca2+ | Strongylocentrotus intermedius | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Strongylocentrotus intermedius | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Strongylocentrotus intermedius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seminal plasma | - |
Strongylocentrotus intermedius | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.5 | - |
- |
Strongylocentrotus intermedius |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for more than 6 months | Strongylocentrotus intermedius |
| 0°C, without freezing, 72 h, inactivation | Strongylocentrotus intermedius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl sulfate + H2O | - |
Strongylocentrotus intermedius | 4-nitrophenol + sulfate | - |
? | |
| ascorbic acid 2-sulfate + H2O | - |
Strongylocentrotus intermedius | ascorbic acid + sulfate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
40 min, 70% loss of activity | Strongylocentrotus intermedius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Strongylocentrotus intermedius |
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