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Literature summary for 3.1.6.1 extracted from

  • Miura, T.; Okamoto, K.; Yanase, H.
    Characterization of arylsulfatase formed by derepressed synthesis in Citrobacter braakii (2006), Biosci. Biotechnol. Biochem., 70, 1509-1512.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA 10 mM, 114% of initial activity Citrobacter braakii

Inhibitors

Inhibitors Comment Organism Structure
iodoacetic acid 10 mM, 48% residual activity Citrobacter braakii
KCN 10 mM, 23% residual activity Citrobacter braakii
Na2B4O7 10 mM, 12% residual activity Citrobacter braakii
sodium dodecylsulfate 1 mM, 4% residual activity Citrobacter braakii
tyramine 10 mM, 14% residual activity Citrobacter braakii
ZnCl2 10 mM, 10% residual activity Citrobacter braakii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6
-
4-nitrocatechol sulfate pH 7.5, 45°C Citrobacter braakii

Organism

Organism UniProt Comment Textmining
Citrobacter braakii
-
marked derepression of enzyme in synthetic medium by addition of tyramine
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-indoxyl sulfate + H2O 6.4% of the activity with 4-nitrophenyl sulfate Citrobacter braakii 1H-indol-3-ol + sulfate
-
?
4-nitrocatechol sulfate + H2O 77% of the activity with 4-nitrophenyl sulfate Citrobacter braakii 4-nitrocatechol + sulfate
-
?
4-nitrophenyl sulfate + H2O
-
Citrobacter braakii 4-nitrophenol + sulfate
-
?
additional information no substrate: 5-bromo-4-chloro-3-indolyl sulfate, phenolphthalein disulfate, galactose 6-sulfate Citrobacter braakii ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Citrobacter braakii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
-
14% residual activity Citrobacter braakii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
3 h, stable up to Citrobacter braakii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Citrobacter braakii

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Citrobacter braakii