Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, 114% of initial activity | Citrobacter braakii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetic acid | 10 mM, 48% residual activity | Citrobacter braakii | |
KCN | 10 mM, 23% residual activity | Citrobacter braakii | |
Na2B4O7 | 10 mM, 12% residual activity | Citrobacter braakii | |
sodium dodecylsulfate | 1 mM, 4% residual activity | Citrobacter braakii | |
tyramine | 10 mM, 14% residual activity | Citrobacter braakii | |
ZnCl2 | 10 mM, 10% residual activity | Citrobacter braakii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
4-nitrocatechol sulfate | pH 7.5, 45°C | Citrobacter braakii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter braakii | - |
marked derepression of enzyme in synthetic medium by addition of tyramine | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-indoxyl sulfate + H2O | 6.4% of the activity with 4-nitrophenyl sulfate | Citrobacter braakii | 1H-indol-3-ol + sulfate | - |
? | |
4-nitrocatechol sulfate + H2O | 77% of the activity with 4-nitrophenyl sulfate | Citrobacter braakii | 4-nitrocatechol + sulfate | - |
? | |
4-nitrophenyl sulfate + H2O | - |
Citrobacter braakii | 4-nitrophenol + sulfate | - |
? | |
additional information | no substrate: 5-bromo-4-chloro-3-indolyl sulfate, phenolphthalein disulfate, galactose 6-sulfate | Citrobacter braakii | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Citrobacter braakii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
14% residual activity | Citrobacter braakii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
3 h, stable up to | Citrobacter braakii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Citrobacter braakii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Citrobacter braakii |