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Information on EC 3.1.3.27 - phosphatidylglycerophosphatase
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3.1.3.27 phosphatidylglycerophosphataseSpecify your search results
Word Map
- 3.1.3.27
-
kallikrein
-
kininogen
-
coagulation
-
hageman
-
fletcher
-
kinin
- thromboplastin
-
fibrinolysis
-
kaolin
-
amidolytic
-
prekallikrein
- fxiia
-
surface-mediated
-
fitzgerald
-
kininase
-
prekallikrein-deficient
-
hmw-kininogen
- synthesis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
ptpmt1, pgp phosphatase, pgp-c, phosphatidylglycerophosphate phosphohydrolase, pgp-a, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
PGP phosphatase
PGPase
PgpB
phosphatidylglycero phosphate phosphatase
-
-
-
-
phosphatidylglycerophosphate phosphatase B
PGP phosphatase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
phosphatidylglycerophosphate + H2O = phosphatidylglycerol + phosphate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
phosphatidylglycerophosphate phosphohydrolase
-
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CAS REGISTRY NUMBER
COMMENTARY
9033-46-9
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
dipalmitoyl sn-glycero 3-phosphate + H2O
dipalmitoyl sn-glycerol + phosphate
-
-
-
r
phosphatidylglycerophosphate + H2O
3-phosphatidylglycerol + phosphate
-
-
-
?
1,2-dioleoyl-sn-glycero-3-phosphate + H2O
1,2-dioleoyl-sn-glycerol + phosphate
C6EFV6
highest activity
-
-
?
1,2-dioleoyl-sn-glycero-3-phosphate + H2O
1,2-dioleoyl-sn-glycerol + phosphate
Escherichia coli B / ATCC 11303
C6EFV6
highest activity
-
-
?
1-oleoyl-2-lyso-sn-glycero-3-phosphate + H2O
1-oleoyl-2-lyso-sn-glycerol + phosphate
C6EFV6
-
-
-
?
1-oleoyl-2-lyso-sn-glycero-3-phosphate + H2O
1-oleoyl-2-lyso-sn-glycerol + phosphate
Escherichia coli B / ATCC 11303
C6EFV6
-
-
-
?
phosphatidylglycerol 3-phosphate + H2O
phosphatidylglycerol + phosphate
-
-
-
-
?
phosphatidylglycerol 3-phosphate + H2O
phosphatidylglycerol + phosphate
-
-
-
-
?
phosphatidylglycerol 3-phosphate + H2O
phosphatidylglycerol + phosphate
-
-
-
-
?
phosphatidylglycerophosphate + H2O
?
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
Micrococcus cerificans
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
-
hydrolysis of other simple phosphomonoesters not catalyzed
-
-
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
-
-
-
r
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
Micrococcus cerificans
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
-
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
-
-
-
r
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
-
-
-
-
?
phosphatidylglycerophosphate + H2O
?
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
Micrococcus cerificans
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
-
biosynthesis of phospholipids
-
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
each protomer contains an independent active site with two metal ions, Ca2+ and Mg2+, forming a heterobinuclear center located in a hydrophilic cavity near the surface of the molecule
Mg2+
Mg2+
-
each protomer contains an independent active site with two metal ions, Ca2+ and Mg2+, forming a heterobinuclear center located in a hydrophilic cavity near the surface of the molecule
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NaF
-
80% loss of activity with 5 mM in compound fraction of PGP-A, PGP-B and PGP-C
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.3
1,2-dioleoyl-sn-glycero-3-phosphate
C6EFV6
pH and temperature not specified in the publication
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
24
1,2-dioleoyl-sn-glycero-3-phosphate
C6EFV6
pH and temperature not specified in the publication
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6
-
PGPA-tase, more specific for phosphatidylglycerolphosphate than PGPB-tase
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
weak association to the mitochondrial membrane
brenda
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
-
phosphatidylglycerophosphate phosphatase essential for the biosynthesis of cardiolipin, an integral component of mitochondrial and bacterial membranes
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PDB
SCOP
CATH
UNIPROT
ORGANISM
Bacillus subtilis (strain 168)
Neisseria meningitidis serogroup B (strain MC58)
Neisseria meningitidis serogroup B (strain MC58)
Neisseria meningitidis serogroup B (strain MC58)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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CRYSTALLIZATION/commentary
ORGANISM
UNIPROT
LITERATURE
with precipitant containing 30% pentaerythritol ethoxylate, 50 mM ammonium sulfate, 50 mM Bis-Tris at pH 6.5
-
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
heat labile
PGP-C more sensitive to increasing temperature
sensitive to sulfhydryl reagents and freeze-thaw cycles
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, for weeks in 2% Triton X-100, 50 mM MgCl2, 20% glycerol, 0.5 M KCl
-
4°C, stable for a number of weeks in 20% glycerol, 0.01 M Tris-HCl, pH 7.5, 0.1 mM EDTA, 50 mM beta-mercaptoethanol
-
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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
partial
purified in a detergent combination of nonyl-beta-D-glucopyranoside and n-dodecyl-N,N-dimethylamine-N-oxide
C6EFV6
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CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dutt, A.; Dowhan, W.
Intracellular distribution of enzymes of phospholipid metabolism in several gram-negative bacteria
J. Bacteriol.
132
159-165
1977
Escherichia coli, Klebsiella aerogenes, Micrococcus cerificans, Salmonella enterica subsp. enterica serovar Typhimurium, Serratia marcescens
brenda
Cain, B.D.; Donohue, T.J.; Shepherd, W.D.; Kaplan, S.
Localization of phospholipid biosynthetic enzyme activities in cell-free fractions derived from Rhodopseudomonas sphaeroides
J. Biol. Chem.
259
942-948
1984
Escherichia coli
brenda
Griebau, R.; Frentzen , M.
Biosynthesis of phosphatidylglycerol in isolated mitochondria of etiolated mung bean (Vigna radiata) seedlings
Plant Physiol.
105
1269-1274
1994
Vigna radiata
brenda
Cao, S.G.; Hatch, G.M.
Stimulation of phosphatidylglycerophosphate phosphatase activity by unsaturated fatty acids in rat heart
Lipids
29
475-480
1994
Rattus sp.
brenda
Dowhan, W.; Funk, C.R.
Phosphatidylglycerophosphate phosphatase from Escherichia coli
Methods Enzymol.
209
224-230
1992
Escherichia coli
brenda
Funk, C.R.; Zimniak, L.; Dowhan, W.
The pgpA and pgpB genes of Escherichia coli are not essential: evidence for a third phosphatidylglycerophosphate phosphatase
J. Bacteriol.
174
205-213
1992
Escherichia coli
brenda
Kelly, B.L.; Greenberg, M.L.
Characterization and regulation of phosphatidylglycerophosphate phosphatase in Saccharomyces cerevisiae
Biochim. Biophys. Acta
1046
144-150
1990
Saccharomyces cerevisiae
brenda
Chiu, T.; Yeh, L.L.
An improved preparation of phosphatidylglycerophosphate
Biochim. Biophys. Acta
876
167-169
1986
Streptococcus sanguinis
brenda
Icho, T.; Raetz, C.R.H.
Multiple genes for membrane-bound phosphatases in Escherichia coli and their action on phospholipid precursors
J. Bacteriol.
153
722-730
1983
Rattus sp.
brenda
Lipton, J.H.; McMurray, W.C.
Phosphatidyl glycerol phosphate phosphatase in BHK-21 cells. A comparison with rat liver mitochondria
Biochem. Biophys. Res. Commun.
73
300-305
1976
Mesocricetus auratus, Rattus sp.
brenda
Larson, T.J.; Hirabayashi, T.; Dowhan, W.
Phosphatidylglycerol biosynthesis in Bacillus licheniformis. Resolution of membrane-bound enzymes by affinity chromatography on cytidinediphospho-sn-1,2-diacylglycerol sepharose
Biochemistry
15
974-979
1976
Bacillus licheniformis
brenda
Chang, Y.; Kennedy, E.P.
Phosphatidyl glycerophosphate phosphatase
J. Lipid Res.
8
456-462
1967
Escherichia coli
brenda
Wu, F.; Yang, Z.; Kuang, T.
Impaired photosynthesis in phosphatidylglycerol-deficient mutant of cyanobacterium Anabaena sp. PCC7120 with a disrupted gene encoding a putative phosphatidylglycerophosphatase
Plant Physiol.
141
1274-1283
2006
Anabaena sp., Anabaena sp. PCC 7120
brenda
Kumaran, D.; Bonanno, J.B.; Burley, S.K.; Swaminathan, S.
Crystal structure of phosphatidylglycerophosphatase (PGPase), a putative membrane-bound lipid phosphatase, reveals a novel binuclear metal binding site and two 'proton wires'
Proteins
64
851-862
2006
Listeria monocytogenes
brenda
Teh, P.G.; Chen, M.J.; Engel, J.L.; Worby, C.A.; Manning, G.; Dixon, J.E.; Zhang, J.
Identification of a mammalian-type phosphatidylglycerophosphate phosphatase in the Eubacterium Rhodopirellula baltica
J. Biol. Chem.
288
5176-5185
2013
Rhodopirellula baltica
brenda
Fan, J.; Jiang, D.; Zhao, Y.; Liu, J.; Zhang, X.C.
Crystal structure of lipid phosphatase Escherichia coli phosphatidylglycerophosphate phosphatase B
Proc. Natl. Acad. Sci. USA
111
7636-7640
2014
Escherichia coli (C6EFV6), Escherichia coli, Escherichia coli B / ATCC 11303 (C6EFV6)
brenda
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