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Literature summary for 3.1.3.27 extracted from
- Phosphatidylglycerol biosynthesis in Bacillus licheniformis. Resolution of membrane-bound enzymes by affinity chromatography on cytidinediphospho-sn-1,2-diacylglycerol sepharose (1976), Biochemistry, 15, 974-979.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | - |
Bacillus licheniformis |  |
General Stability
| General Stability | Organism |
|---|---|
| 50% loss of activity after solubilization with Triton X-100 in 12 h | Bacillus licheniformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | high concentration | Bacillus licheniformis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
phosphatidylglycerophosphate | - |
Bacillus licheniformis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic membrane | - |
Bacillus licheniformis | - |
- |
| membrane | - |
Bacillus licheniformis | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | addition of high concentrated divalent cations not required | Bacillus licheniformis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylglycerophosphate + H2O | Bacillus licheniformis | biosynthesis of phospholipids | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bacillus licheniformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0023 | - |
partially purified | Bacillus licheniformis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, for weeks in 2% Triton X-100, 50 mM MgCl2, 20% glycerol, 0.5 M KCl | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylglycerophosphate + H2O | - |
Bacillus licheniformis | 3-phosphatidylglycerol + phosphate | - |
? | |
| phosphatidylglycerophosphate + H2O | biosynthesis of phospholipids | Bacillus licheniformis | ? | - |
? | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | - |
Bacillus licheniformis |
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Release 2023.1 (January 2023)
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