Literature summary for 3.1.3.27 extracted from

Ghachi, M.E.; Howe, N.; Auger, R.; Lambion, A.; Guiseppi, A.; Delbrassine, F.; Manat, G.; Roure, S.; Peslier, S.; Sauvage, E.; Vogeley, L.; Rengifo-Gonzalez, J.C.; Charlier, P.; Mengin-Lecreulx, D.; Foglino, M.; Touze, T.; Caffrey, M.; Kerff, F.
Crystal structure and biochemical characterization of the transmembrane PAP2 type phosphatidylglycerol phosphate phosphatase from Bacillus subtilis (2017), Cell. Mol. Life Sci., 74, 2319-2332 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
structure at 2.25 A resolution, with phosphate analogue tungstate in the active site. The structure reveals two lipid chains in the active site vicinity	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
G86F	no significant change in activity	Bacillus subtilis
K93A	no significant change in activity with 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate), more than 50% increase in activity for undecaprenyldiphosphate and farnesyldiphosphate	Bacillus subtilis
K93W	no significant change in activity	Bacillus subtilis
L82W	no significant change in activity	Bacillus subtilis
R89A	slight decrease in activity	Bacillus subtilis
S55A	no significant change in activity	Bacillus subtilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Bacillus subtilis	16020	-

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	O34349	-	-
Bacillus subtilis 168	O34349	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
12.5	-	substrate undecaprenyldiphosphate, pH 7.4, 37°C	Bacillus subtilis
19.8	-	substrate isopentenyldiphosphate, pH 7.4, 37°C	Bacillus subtilis
30.6	-	substrate farnesyldiphosphate, pH 7.4, 37°C	Bacillus subtilis
54.7	-	substrate 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate), pH 7.4, 37°C	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + H2O	-	Bacillus subtilis	1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + phosphate	-	?
1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + H2O	-	Bacillus subtilis 168	1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + phosphate	-	?
farnesyldiphosphate + H2O	-	Bacillus subtilis	farnesylphosphate + phosphate	-	?
farnesyldiphosphate + H2O	-	Bacillus subtilis 168	farnesylphosphate + phosphate	-	?
isopentenyldiphosphate + H2O	-	Bacillus subtilis	isopentenylphosphate + phosphate	-	?
isopentenyldiphosphate + H2O	-	Bacillus subtilis 168	isopentenylphosphate + phosphate	-	?
undecaprenyldiphosphate + H2O	-	Bacillus subtilis	undecaprenylphosphate + phosphate	-	?
undecaprenyldiphosphate + H2O	-	Bacillus subtilis 168	undecaprenylphosphate + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
PgpB	-	Bacillus subtilis
YodM	-	Bacillus subtilis

General Information

General Information	Comment	Organism
physiological function	enzyme is the principal phosphatidylglycerol phosphate phosphatase of Bacillus subtilis. Gene expression complements both undecaprenylphosphate and phosphatidylglycerol metabolisms in Escherichia coli mutant strains	Bacillus subtilis

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Release 2023.1 (January 2023)