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Literature summary for 3.1.3.27 extracted from

  • Tong, S.; Lin, Y.; Lu, S.; Wang, M.; Bogdanov, M.; Zheng, L.
    Structural insight into substrate selection and catalysis of lipid phosphate phosphatase PgpB in the cell membrane (2016), J. Biol. Chem., 291, 18342-18352 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
pgpB gene is cloned into the pET22b vector (Novagen) to generate the C-terminal His6-tagged protein. Mutants were generated using standard site-directed mutagenesis. The PgpB wild type and mutant proteins are expressed in Escherichia coli strain C41(DE3) Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion method, the structure is determined at 3.2 A resolution Escherichia coli

Protein Variants

Protein Variants Comment Organism
D211A mutant enzyme retains 50% activity Escherichia coli
H163A mutation increases hydrolysis of 1,2-dipalmitoryl-sn-glycero-3-[phospho-rac-(1'-(3'-phospho)glycerol)] by 50% Escherichia coli
K93A the mutant enzyme shows complete activity loss for lysophosphatidic acid and (2S,3R,4E)-2-aminooctadec-4-ene-1,3-diol-1-phosphate, whereas it retains full activity for phosphatidic acid and gains 40% more activity toward phosphatidylglycerol phosphate compared with wild type enzyme Escherichia coli
K93A/K97A the mutant enzyme shows complete activity loss for lysophosphatidic acid and (2S,3R,4E)-2-aminooctadec-4-ene-1,3-diol-1-phosphate, whereas it retains full activity for phosphatidic acid and gained 40% more activity toward phosphatidylglycerol phosphate compared with wild type enzyme Escherichia coli
K93T mutant enzyme with significantly improved activity for lysophosphatidic acid, (2S,3R,4E)-2-aminooctadec-4-ene-1,3-diol-1-phosphate, and phosphatidic acid by 2.5- or 3.4-fold but not for 1,2-dipalmitoryl-sn-glycero-3-[phospho-rac-(1'-(3'-phospho)glycerol)] Escherichia coli
K93T/K97A the mutant enzyme loses all activity for lysophosphatidic acid or (2S,3R,4E)-2-aminooctadec-4-ene-1,3-diol-1-phosphate, but retains 50% activity for phosphatidic acid or 1,2-dipalmitoryl-sn-glycero-3-[phospho-rac-(1'-(3'-phospho)glycerol)] Escherichia coli
K97A the mutant enzyme shows complete activity loss for lysophosphatidic acid and (2S,3R,4E)-2-aminooctadec-4-ene-1,3-diol-1-phosphate, whereas it retains full activity for phosphatidic acid and gained 40% more activity toward phosphatidylglycerol phosphate compared with wild type enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
orthovanadate
-
Escherichia coli
phosphatidylethanolamine 3 mM, 70% inhibition. competitive Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.108
-
1-(9Z-octadecenoyl)-sn-glycero-3-phosphate pH 7.5, 23°C Escherichia coli
5.052
-
1-hexanoyl-sn-glycero-3-phosphate pH 7.5, 23°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane protein Escherichia coli 16020
-

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A924
-
-
Escherichia coli K12 P0A924
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S,3R,4E)-2-aminooctadec-4-ene-1,3-diol-1-phosphate + H2O
-
Escherichia coli ?
-
?
(2S,3R,4E)-2-aminooctadec-4-ene-1,3-diol-1-phosphate + H2O
-
Escherichia coli K12 ?
-
?
1,2-dihexadecanoyl-sn-glycero-3-phosphate + H2O
-
Escherichia coli 1,2-dihexadecanoyl-sn-glycerol + phosphate
-
?
1,2-dihexadecanoyl-sn-glycero-3-phosphate + H2O
-
Escherichia coli K12 1,2-dihexadecanoyl-sn-glycerol + phosphate
-
?
1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1'-(3'-phospho)glycerol)] + H2O
-
Escherichia coli ? + phosphate
-
?
1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1'-(3'-phospho)glycerol)] + H2O
-
Escherichia coli K12 ? + phosphate
-
?
1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H2O
-
Escherichia coli 1-(9Z-octadecenoyl)-sn-glycerol + phosphate
-
?
1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H2O
-
Escherichia coli K12 1-(9Z-octadecenoyl)-sn-glycerol + phosphate
-
?
1-hexanoyl-sn-glycero-3-phosphate + H2O
-
Escherichia coli 1-hexanoyl-sn-glycerol + phosphate
-
?
1-hexanoyl-sn-glycero-3-phosphate + H2O
-
Escherichia coli K12 1-hexanoyl-sn-glycerol + phosphate
-
?

Synonyms

Synonyms Comment Organism
PgpB
-
Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7
-
phosphatidylethanolamine pH 7.5, 23°C Escherichia coli