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Various 2-acylglycerols can act as acceptor; palmitoyl-CoA and other long-chain acyl-CoAs can act as donors. The sn-1 position and the sn-3 position are both acylated, at about the same rate.
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
acyl CoA:monoacylglycerol acyltransferase, acyl CoA:monoacylglycerol acyltransferase-2, acyl coenzyme A-monoglyceride acyltransferase, acyl coenzyme A:diacylglycerol acyltransferase, acyl-CoA:monoacylglycerol acyltransferase, acyl-CoA:monoacylglycerol transferase, acyl-coenzyme A:monoacylglycerol acyltransferase, acylglycerol palmitoyltransferase, DGAT, DGAT1/ARAT, more
acyl-CoA + 2-acylglycerol = CoA + diacylglycerol