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Literature summary for 2.3.1.22 extracted from
- Identification, purification, and characterization of monoacylglycerol acyltransferase from developing peanut cotyledons (2001), J. Biol. Chem., 276, 10847-10852.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| oleic acid | stimulates below 0.015 mM | Arachis hypogaea |  |
| palmitic acid | 0.02-0.05 mM, stimulates | Arachis hypogaea | |
| phosphatidic acid | stimulates | Arachis hypogaea | |
| phosphatidylcholine | stimulates | Arachis hypogaea | |
| sphingomyelin | 0.0025-0.0075 mM, activates | Arachis hypogaea | |
| sphingosine | 0.0025-0.0075 mM, activates | Arachis hypogaea | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-octylglucoside | 40 mM, 50% loss of activity | Arachis hypogaea | |
| CHAPS | 20 mM, complete loss of activity | Arachis hypogaea | |
| NaF | - |
Arachis hypogaea | |
| Triton X-100 | 42% loss of activity | Arachis hypogaea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00565 | - |
1-oleoyl-sn-glycerol | - |
Arachis hypogaea | |
| 0.00935 | - |
oleoyl-CoA | - |
Arachis hypogaea | |
| 0.01639 | - |
1-palmitoylglycerol | - |
Arachis hypogaea | |
| 0.01754 | - |
palmitoyl-CoA | - |
Arachis hypogaea | |
| 0.02566 | - |
stearoyl-CoA | - |
Arachis hypogaea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
gel filtration | Arachis hypogaea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + sn-2-monoacylglycerol | Arachis hypogaea | diacylglycerol biosynthetic activity | CoA + diacylglycerol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arachis hypogaea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Arachis hypogaea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Arachis hypogaea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0159 | - |
- |
Arachis hypogaea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + sn-2-monoacylglycerol | diacylglycerol biosynthetic activity | Arachis hypogaea | CoA + diacylglycerol | - |
? | |
| oleoyl-CoA + 1-oleoyl-sn-glycerol | - |
Arachis hypogaea | CoA + 1,2-dioleoyl-sn-glycerol | - |
? | |
| oleoyl-CoA + 1-palmitoyl-sn-glycerol | - |
Arachis hypogaea | CoA + 1-palmitoyl-3-oleoyl-sn-glycerol | - |
? | |
| palmitoyl-CoA + 1-palmitoyl-sn-glycerol | - |
Arachis hypogaea | CoA + ? | - |
? | |
| palmitoyl-CoA + sn-1-monooleoylglycerol | - |
Arachis hypogaea | CoA + ? | - |
? | |
| stearoyl-CoA + 1-palmitoyl-sn-glycerol | - |
Arachis hypogaea | CoA + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Arachis hypogaea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Arachis hypogaea |
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