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Information on EC 1.2.1.32 - aminomuconate-semialdehyde dehydrogenase
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1.2.1.32 aminomuconate-semialdehyde dehydrogenaseIUBMB Comments
Also acts on 2-hydroxymuconate semialdehyde.
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Word Map
- 1.2.1.32
- catechols
- putida
-
meta-cleavage
-
hydratase
- 4-oxalocrotonate
- 2-aminophenols
- toluate
- m-cresol
- 3-methylcatechol
- 4-hydroxy-2-oxovalerate
- nitrobenzene
- comamonas
-
xylf
-
extradiol
-
chloroaromatics
-
isofunctional
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
2-hydroxymuconic semialdehyde dehydrogenase, aldh12, amsdh, 2-hydroxymuconate semialdehyde dehydrogenase, 2-aminomuconic 6-semialdehyde dehydrogenase, 2-aminomuconate-6-semialdehyde dehydrogenase, 2-ams dehydrogenase, 2-hydroxymuconic 6-semialdehyde dehydrogenase, 2-hydroxymuconic acid semialdehyde dehydrogenase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2-aminomuconate semialdehyde dehydrogenase
2-aminomuconate-6-semialdehyde dehydrogenase
2-aminomuconic 6-semialdehyde dehydrogenase
-
-
-
-
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate dehydrogenase
-
-
-
-
2-hydroxymuconate semialdehyde dehydrogenase
-
-
-
-
2-hydroxymuconic 6-semialdehyde dehydrogenase
2-hydroxymuconic acid semialdehyde dehydrogenase
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-
-
-
2-hydroxymuconic semialdehyde dehydrogenase
alpha-aminomuconic epsilon-semialdehyde dehydrogenase
-
-
-
-
alpha-hydroxymuconic epsilon-semialdehyde dehydrogenase
-
-
-
-
AMDH
aminomuconate semialdehyde dehydrogenase
-
-
-
-
aminomuconate-semialdehyde dehydrogenase
AMSDH
dehydrogenase, 2-hydroxymuconate semialdehyde
-
-
-
-
dehydrogenase, aminomuconate semialdehyde
-
-
-
-
nbaE
2-hydroxymuconic semialdehyde dehydrogenase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2-aminomuconate 6-semialdehyde + NAD+ + H2O = 2-aminomuconate + NADH + H+
-
-
-
-
2-aminomuconate 6-semialdehyde + NAD+ + H2O = 2-aminomuconate + NADH + H+
the catalytic cycle, overview
2-aminomuconate 6-semialdehyde + NAD+ + H2O = 2-aminomuconate + NADH + H+
the catalytic cycle, overview
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
oxidation
-
-
-
-
reduction
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
2-aminomuconate-6-semialdehyde:NAD+ 6-oxidoreductase
Also acts on 2-hydroxymuconate semialdehyde.
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CAS REGISTRY NUMBER
COMMENTARY
37250-95-6
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-aminomuconic acid semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + H+
-
-
-
-
?
2-hydroxymuconate 6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + 2 H+
-
-
-
?
2-hydroxymuconic 6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
substrate for spectrophotometric activity assay, relative activity 100%
-
-
?
3-chlorobenzaldehyde + NAD+ + H2O
3-chlorobenzoate + NADH
-
low activity
-
-
?
3-fluorobenzaldehyde + NAD+ + H2O
3-fluorobenzoate + NADH + H+
-
low activity
-
-
?
3-methoxybenzaldehyde + NAD+ + H2O
3-methoxybenzoate + NADH
-
low activity
-
-
?
3-methylbenzaldehyde + NAD+ + H2O
3-methylbenzoate + NADH
-
low activity
-
-
?
4-methoxybenzaldehyde + NAD+ + H2O
4-methoxybenzoate + NADH + H+
-
low activity
-
-
?
4-methylbenzaldehyde + NAD+ + H2O
4-methylbenzoate + NADH
-
low activity
-
-
?
4-nitrobenzaldehyde + NAD+ + H2O
4-nitrobenzoate + NADH + H+
-
low activity
-
-
?
pyridine 4-carboxyaldehyde + NAD+ + H2O
pyridine 4-carboxylate + NADH
-
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + 2 H+
-
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + 2 H+
-
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + 2 H+
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + 2 H+
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + 2 H+
enzyme-substrate binding structure and catalytic mechanism, overview. Due to the unstable nature of its substrate 2-aminomuconate 6-semialdehyde, 2-AMS, the activity of AMSDH is detected using a coupled-enzyme assay that employs its upstream partner, alpha-amino beta-carboxymuconate epsilon-semialdehyde decarboxylase (ACMSD), to generate 2-AMS in situ
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-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + 2 H+
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + 2 H+
enzyme-substrate binding structure and catalytic mechanism, overview. Due to the unstable nature of its substrate 2-aminomuconate 6-semialdehyde, 2-AMS, the activity of AMSDH is detected using a coupled-enzyme assay that employs its upstream partner, alpha-amino beta-carboxymuconate epsilon-semialdehyde decarboxylase (ACMSD), to generate 2-AMS in situ
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-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + H+
-
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + H+
-
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + H+
-
-
-
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
-
-
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
-
metabolism of benzene ring of tryptophan in mammalian tissues
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
-
-
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
-
-
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
-
catabolism of nitrobenzene as sole source of carbon, nitrogen and energy
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
-
catabolism of nitrobenzene as sole source of carbon, nitrogen and energy
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
-
-
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
-
catabolism of nitrobenzene as sole source of carbon, nitrogen and energy
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
-
-
-
?
2-fluorobenzaldehyde + NAD+ + H2O
2-fluorobenzoate + NADH + H+
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low activity
-
-
?
2-fluorobenzaldehyde + NAD+ + H2O
2-fluorobenzoate + NADH + H+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
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low activity
-
-
?
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O
2-hydroxy-5-methylmuconate + NADH
-
synonym 5-methyl-2-hydroxymuconic-6-semialdehyde
-
-
?
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O
2-hydroxy-5-methylmuconate + NADH
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synonym 5-methyl-2-hydroxymuconic-6-semialdehyde
-
-
?
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O
2-hydroxy-5-methylmuconate + NADH
-
synonym 5-methyl-2-hydroxymuconic-6-semialdehyde
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-
?
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O
2-hydroxy-5-methylmuconate + NADH
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synonym 5-methyl-2-hydroxymuconic-6-semialdehyde
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-
?
2-hydroxy-5-methyl-muconic-6-semialdehyde + NAD+
2-hydroxy-5-methylmuconate + NADH
most effective substrate
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-
?
2-hydroxy-5-methyl-muconic-6-semialdehyde + NAD+
2-hydroxy-5-methylmuconate + NADH
most effective substrate
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-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
-
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
-
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
-
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
-
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
4-oxalocrotonate + NADH + H+
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
4-oxalocrotonate + NADH + H+
involved in meta cleavage pathway of aromatic hydrocarbons
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
4-oxalocrotonate + NADH + H+
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
4-oxalocrotonate + NADH + H+
involved in meta cleavage pathway of aromatic hydrocarbons
-
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
-
-
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
-
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
-
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
dissimilatory meta-cleavage of catechols
-
?
3-nitrobenzaldehyde + NAD+ + H2O
3-nitrobenzoate + NADH
-
low activity
-
-
?
4-chlorobenzaldehyde + NAD+ + H2O
4-chlorobenzoate + NADH
-
low activity
-
-
?
4-chlorobenzaldehyde + NAD+ + H2O
4-chlorobenzoate + NADH
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
low activity
-
-
?
4-fluorobenzaldehyde + NAD+ + H2O
4-fluorobenzoate + NADH + H+
-
low activity
-
-
?
4-fluorobenzaldehyde + NAD+ + H2O
4-fluorobenzoate + NADH + H+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
low activity
-
-
?
5-chloro-2-hydroxymuconic-6-semialdehyde + NAD+
5-chloro-2-hydroxymuconate + NADH
poor substrate
-
-
?
5-chloro-2-hydroxymuconic-6-semialdehyde + NAD+
5-chloro-2-hydroxymuconate + NADH
poor substrate
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
-
relative activity 113%
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
-
low activity
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
-
low activity
-
-
?
butyraldehyde + NAD+ + H2O
butyrate + NADH + H+
-
relative activity 138%
-
-
?
butyraldehyde + NAD+ + H2O
butyrate + NADH + H+
-
relative activity 138%
-
-
?
crotonaldehyde + NAD+ + H2O
crotonate + NADH
-
low activity
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
-
relative activity 107%
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
-
relative activity 107%
-
-
?
additional information
?
-
no activity with 2-hydroxy-6-oxo-hepta-2,4-dienoic acid and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
-
?
additional information
?
-
-
no activity with 2-hydroxy-6-oxo-hepta-2,4-dienoic acid and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
-
?
additional information
?
-
no activity with 2-hydroxy-6-oxo-hepta-2,4-dienoic acid and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
-
?
additional information
?
-
-
no activity with 2-hydroxy-6-oxo-hepta-2,4-dienoic acid and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
-
?
additional information
?
-
-
no reaction with 2-hydroxybenzaldehyde, 3-hydroxybenzaldehyde
-
-
?
additional information
?
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
no reaction with 2-methylbenzaldehyde
-
-
?
additional information
?
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
no reaction with 2-chlorobenzaldehyde
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
-
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + 2 H+
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + 2 H+
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + 2 H+
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + H+
-
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + H+
-
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + H+
-
-
-
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
-
metabolism of benzene ring of tryptophan in mammalian tissues
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
-
catabolism of nitrobenzene as sole source of carbon, nitrogen and energy
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
-
catabolism of nitrobenzene as sole source of carbon, nitrogen and energy
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
-
catabolism of nitrobenzene as sole source of carbon, nitrogen and energy
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
4-oxalocrotonate + NADH + H+
involved in meta cleavage pathway of aromatic hydrocarbons
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
4-oxalocrotonate + NADH + H+
involved in meta cleavage pathway of aromatic hydrocarbons
-
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
-
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
-
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
-
dissimilatory meta-cleavage of catechols
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
enzyme-cofactor binding structure and catalytic mechanism, overview
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
CuSO4
-
0% remaining activity at an inhibitor concentration of 1 mM; 100% inhibition at 1 mM
HgCl2
-
0% remaining activity at an inhibitor concentration of 1 mM; 100% inhibition at 1 mM
iodoacetamide
-
equimolar amount reduces benzaldehyde oxidation activity by 44%
iodoacetic acid
-
31% inhibition at 1 mM; 69% remaining activity at an inhibitor concentration of 1 mM
p-chloromercuribenzoic acid
-
41% remaining activity at an inhibitor concentration of 1 mM; 59% inhibition at 1 mM
ZnSO4
-
26% remaining activity at an inhibitor concentration of 1 mM; 74% inhibition at 1 mM