Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli HB101 | Burkholderia cepacia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
2-hydroxy-5-methyl-muconic-6-semialdehyde | pH 8.5, 25°C | Burkholderia cepacia | |
0.011 | - |
2-hydroxymuconate-6-semialdehyde | pH 8.5, 25°C | Burkholderia cepacia | |
0.046 | - |
NAD+ | pH 8.5, 25°C, with 2-hydroxy-5-methyl-muconic-6-semialdehyde as substrate | Burkholderia cepacia | |
0.083 | - |
5-chloro-2-hydroxymuconic-6-semialdehyde | pH 8.5, 25°C | Burkholderia cepacia | |
0.294 | - |
NAD+ | pH 8.5, 25°C, with 2-hydroxymuconate-6-semialdehyde as substrate | Burkholderia cepacia | |
1.316 | - |
NAD+ | pH 8.5, 25°C, with 5-chloro-2-hydroxymuconic-6-semialdehyde as substrate | Burkholderia cepacia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51740 | - |
calculated from the predicted amino acid sequence | Burkholderia cepacia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O | Burkholderia cepacia | involved in meta cleavage pathway of aromatic hydrocarbons | 4-oxalocrotonate + NADH + H+ | - |
? | |
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O | Burkholderia cepacia G4 | involved in meta cleavage pathway of aromatic hydrocarbons | 4-oxalocrotonate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | Q83XU8 | formerly Pseudomonas cepacia | - |
Burkholderia cepacia G4 | Q83XU8 | formerly Pseudomonas cepacia | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-5-methyl-muconic-6-semialdehyde + NAD+ | most effective substrate | Burkholderia cepacia | 2-hydroxy-5-methylmuconate + NADH | - |
? | |
2-hydroxy-5-methyl-muconic-6-semialdehyde + NAD+ | most effective substrate | Burkholderia cepacia G4 | 2-hydroxy-5-methylmuconate + NADH | - |
? | |
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O | - |
Burkholderia cepacia | 4-oxalocrotonate + NADH + H+ | - |
? | |
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O | involved in meta cleavage pathway of aromatic hydrocarbons | Burkholderia cepacia | 4-oxalocrotonate + NADH + H+ | - |
? | |
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O | - |
Burkholderia cepacia G4 | 4-oxalocrotonate + NADH + H+ | - |
? | |
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O | involved in meta cleavage pathway of aromatic hydrocarbons | Burkholderia cepacia G4 | 4-oxalocrotonate + NADH + H+ | - |
? | |
5-chloro-2-hydroxymuconic-6-semialdehyde + NAD+ | poor substrate | Burkholderia cepacia | 5-chloro-2-hydroxymuconate + NADH | - |
? | |
5-chloro-2-hydroxymuconic-6-semialdehyde + NAD+ | poor substrate | Burkholderia cepacia G4 | 5-chloro-2-hydroxymuconate + NADH | - |
? | |
additional information | no activity with 2-hydroxy-6-oxo-hepta-2,4-dienoic acid and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | Burkholderia cepacia | ? | - |
? | |
additional information | no activity with 2-hydroxy-6-oxo-hepta-2,4-dienoic acid and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | Burkholderia cepacia G4 | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2300 | - |
5-chloro-2-hydroxymuconic-6-semialdehyde | pH 8.5, 25°C | Burkholderia cepacia | |
21900 | - |
2-hydroxymuconate-6-semialdehyde | pH 8.5, 25°C | Burkholderia cepacia | |
99300 | - |
2-hydroxy-5-methyl-muconic-6-semialdehyde | pH 8.5, 25°C | Burkholderia cepacia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | no activity in the absence of NAD+ | Burkholderia cepacia |