    1.2.1.32 | purified recombinant His-tagged E268A mutant enzyme in complex with NAD+ or NAD+ and 2-aminomuconate or 2-hydroxymuconate, hanging drop diffusion, mixing of 0.0015 ml of 40 mg/ml protein solution with 0.0015 ml of reservoir solution containing 20% PEG 3350 and 0.2 M sodium phosphate dibasic monohydrate, pH 9.1, at 18°C, 2-3 days, crystals are soaked in ligand solution for the formation of complex crystals, X-ray diffraction structure determination and analysis at 1.95-2.20 A resolution, molecular replacement using 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase, PDB: 2D4E, as a search model, and molecular modelling |
