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Literature summary for 1.2.1.32 extracted from

  • Inoue, J.; Shaw, J.P.; Rekik, M.; Harayama, S.
    Overlapping substrate specificities of benzaldehyde dehydrogenase (the xylC gene product) and 2-hydroxymuconic semialdehyde dehydrogenase (the xylG gene product) encoded by TOL plasmid pWW0 of Pseudomonas putida (1995), J. Bacteriol., 177, 1196-1201.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0093
-
 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate synonym 5-methyl-2-hydroxymuconic-6-semialdehyde Pseudomonas putida
0.017
-
 2-hydroxymuconate-6-semialdehyde
-
 Pseudomonas putida
0.17
-
 3-Fluorobenzaldehyde
-
 Pseudomonas putida
0.33
-
 NAD+
-
 Pseudomonas putida
0.46
-
 benzaldehyde
-
 Pseudomonas putida
0.53
-
 4-methylbenzaldehyde
-
 Pseudomonas putida
0.63
-
 3-methylbenzaldehyde
-
 Pseudomonas putida
0.81
-
 3-Nitrobenzaldehyde
-
 Pseudomonas putida
0.94
-
 3-Methoxybenzaldehyde
-
 Pseudomonas putida
1.3
-
 3-Chlorobenzaldehyde
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-hydroxymuconic acid semialdehyde + NAD+ + H2O Pseudomonas putida
-
 2-hydroxymuconate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
erroneous identification as benzaldehyde dehydrogenase corrected Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O synonym 5-methyl-2-hydroxymuconic-6-semialdehyde Pseudomonas putida 2-hydroxy-5-methylmuconate + NADH
-
 ?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
-
 Pseudomonas putida 2-hydroxymuconate + NADH
-
 ?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
-
 Pseudomonas putida 2-hydroxymuconate + NADH + H+
-
 ?
3-chlorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-chlorobenzoate + NADH
-
 ?
3-fluorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-fluorobenzoate + NADH + H+
-
 ?
3-methoxybenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-methoxybenzoate + NADH
-
 ?
3-methylbenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-methylbenzoate + NADH
-
 ?
3-nitrobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-nitrobenzoate + NADH
-
 ?
4-methylbenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 4-methylbenzoate + NADH
-
 ?
benzaldehyde + NAD+ + H2O low activity Pseudomonas putida benzoate + NADH + H+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas putida