2.4.1.266: glucosyl-3-phosphoglycerate synthase
This is an abbreviated version!
For detailed information about glucosyl-3-phosphoglycerate synthase, go to the full flat file.
Word Map on EC 2.4.1.266
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2.4.1.266
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mycobacteria
-
tuberculosis
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glucosylglycerate
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glycosyltransferase
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lipopolysaccharide
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methylglucose
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gg
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d-3-phosphoglycerate
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gdp-mannose
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planctomycetes
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udp
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phylum
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burtonii
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methylmannose
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marina
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deep-branching
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smegmatis
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mannosyl-3-phosphoglycerate
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methanococcoides
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mobilis
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petrotoga
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gdp-glucose
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glucosylation
- 2.4.1.266
- mycobacteria
- tuberculosis
-
glucosylglycerate
- glycosyltransferase
- lipopolysaccharide
-
methylglucose
- gg
- d-3-phosphoglycerate
- gdp-mannose
- planctomycetes
- udp
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phylum
- burtonii
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methylmannose
- marina
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deep-branching
- smegmatis
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mannosyl-3-phosphoglycerate
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methanococcoides
- mobilis
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petrotoga
- gdp-glucose
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glucosylation
Reaction
Synonyms
glucosyl-3-phosphoglycerate synthase, glucosyl-3-phosphoglycerate synthase/phosphatase, glucosylphosphoglycerate synthase, GpgS, GpgS protein, GpgS/P, More, MSMEG_5084, RB1005, Rv1208 protein
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2.4.1.266 glucosyl-3-phosphoglycerate synthaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.4.1.266 - glucosyl-3-phosphoglycerate synthase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ADP-alpha-D-glucose + D-3-phosphoglycerate
ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
ADP-glucose + 3-phospho-D-glycerate
ADP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
ADP-glucose + 3-phospho-D-glycerate
ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
GDP-glucose + 3-phospho-D-glycerate
GDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
GDP-glucose + 3-phospho-D-glycerate
GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
GDP-glucose + D-3-phosphoglycerate
GDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
-
-
-
?
TDP-glucose + 3-phospho-D-glycerate
TDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
the recombinant GpgS protein of Persephonella marina catalyzes the synthesis of 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate from UDP-glucose, GDP-glucose, ADP-glucose, and TDP-glucose (in order of decreasing efficiency) and from D-3-phosphoglycerate
-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate
in Persephonella marina two pathways for synthesis of glucosylglycerate are present: 1. the single-step pathway in with glucosylglycerate synthase (Ggs) catalyzes the synthesis of 2-O-(alpha-D-glucosyl)-D-glycerate in one-step fop, ADP-glucose and D-glycerate, and 2. the two-step pathway in which glucosyl-3-phosphoglycerate synthase (GpgS) catalyzes the conversion of NDP-glucose and D-3-phosphoglycerate into glucosyl-3-phosphoglycerate, which is then converted to 2-O-(alpha-D-glucosyl)-D-glycerate by glucosyl-3-phosphoglycerate phosphatase (GpgP)
-
-
?
ADP-alpha-D-glucose + D-3-phosphoglycerate
ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
-
?
ADP-alpha-D-glucose + D-3-phosphoglycerate
ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
-
?
ADP-alpha-D-glucose + D-3-phosphoglycerate
ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
-
?
ADP-alpha-D-glucose + D-3-phosphoglycerate
ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
ADP-alpha-D-glucose + D-3-phosphoglycerate
ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
ADP-alpha-D-glucose + D-3-phosphoglycerate
ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
ADP-glucose + 3-phospho-D-glycerate
ADP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
UDP-glucose is the preferred substrate, but it could be partially replaced by ADP-glucose. D-3-phosphoglycerate is the only acceptor for the synthesis of glucosyl-3-phosphoglycerate
-
-
?
ADP-glucose + 3-phospho-D-glycerate
ADP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
ADP-glucose + 3-phospho-D-glycerate
ADP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
ADP-glucose + 3-phospho-D-glycerate
ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
-
?
ADP-glucose + 3-phospho-D-glycerate
ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
ADP-glucose + 3-phospho-D-glycerate
ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
ADP-glucose + 3-phospho-D-glycerate
ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
ADP-glucose + 3-phospho-D-glycerate
ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
the recombinant GpgS protein of Persephonella marina catalyzes the synthesis of 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate from UDP-glucose, GDP-glucose, ADP-glucose, and TDP-glucose (in order of decreasing efficiency) and from D-3-phosphoglycerate
-
-
?
ADP-glucose + 3-phospho-D-glycerate
ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
ADP-glucose + 3-phospho-D-glycerate
ADP + 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate
-
-
-
?
ADP-glucose + 3-phospho-D-glycerate
ADP + 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate
-
-
-
?
GDP-glucose + 3-phospho-D-glycerate
GDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
GDP-glucose + 3-phospho-D-glycerate
GDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
GDP-glucose + 3-phospho-D-glycerate
GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
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-
-
?
GDP-glucose + 3-phospho-D-glycerate
GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP). An mpgS gene coding for mannosyl-3-phosphoglycerate synthase (MpgS) is absent
-
-
?
GDP-glucose + 3-phospho-D-glycerate
GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
-
?
GDP-glucose + 3-phospho-D-glycerate
GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
GDP-glucose + 3-phospho-D-glycerate
GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
GDP-glucose + 3-phospho-D-glycerate
GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
GDP-glucose + 3-phospho-D-glycerate
GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
GDP-glucose + 3-phospho-D-glycerate
GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
the recombinant GpgS protein of Persephonella marina catalyzes the synthesis of 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate from UDP-glucose, GDP-glucose, ADP-glucose, and TDP-glucose (in order of decreasing efficiency) and from D-3-phosphoglycerate
-
-
?
GDP-glucose + 3-phospho-D-glycerate
GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
the enzyme is involved in the phosphorylating pathway for synthesis of the solute mannosylglucosylglycerate. In Petrotoga mobilis two alternative pathways for the synthesis of mannosylglucosylglycerate are proposed. The first one is a a phosphorylating pathway (with a phosphorylated intermediate) from 3-phosphoglycerate and UDP-glucose to the final solute. The second nonphosphorylating pathway (no phosphorylated intermediates) could represent an alternative route for the synthesis of mannosylglucosylglycerate in Petrotoga mobilis that could lead to the direct conversion of glucosylglycerate and GDP-mannose to mannosylglucosylglycerate. Pathway multiplicity likely reflects a crucial role for mannosylglucosylglycerate in the physiology of Petrotoga mobilis mobilis during stress adaptation
-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
UDP-glucose is the preferred substrate, but it could be partially replaced by ADP-glucose. D-3-phosphoglycerate is the only acceptor for the synthesis of glucopyranosyl-3-phosphoglycerate
-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
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-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
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-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
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-
-
-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
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-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
three-dimensional structures provide a molecular explanation for the enzymes preference for UDP-containing donor substrates, as well as for its glucose versus mannose discrimination, and uncover the structural determinants for acceptor substrate selectivity
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-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
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-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
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-
-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
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involved in biosynthesis of methylated polysaccharides
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-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
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-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
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-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
involved in biosynthesis of methylated polysaccharides
-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
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-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
involved in biosynthesis of methylated polysaccharides
-
-
?
UDP-glucose + 3-phospho-D-glycerate
UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
the recombinant GpgS protein of Persephonella marina catalyzes the synthesis of 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate from UDP-glucose, GDP-glucose, ADP-glucose, and TDP-glucose (in order of decreasing efficiency) and from D-3-phosphoglycerate
-
-
?
UDP-glucose + D-3-phosphoglycerate
UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
-
best substrates
-
-
?
UDP-glucose + D-3-phosphoglycerate
UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
-
best substrates
-
-
?
UDP-glucose + D-3-phosphoglycerate
UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
-
best substrates
-
-
?
UDP-glucose + D-3-phosphoglycerate
UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
best substrates
-
-
?
UDP-glucose + D-3-phosphoglycerate
UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
best substrates
-
-
?
UDP-glucose + D-3-phosphoglycerate
UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
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-
-
?
additional information
?
-
no activity with: ADP-ribose, UDP-acetylgalactosamine, UDP-glucuronic acid, UDP-galactose, GDP-fucose, GDP-mannose, UDP-mannose, ADP-mannose, UDP-glucose, TDP-glucose, and ADP-glucose as sugar donors and glycerol, D-2-phosphoglycerate, L-glycerol-3-phosphate, 2,3-diphospho-D-glycerate, and phosphoenolpyruvate
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-
?
additional information
?
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-
no activity with: ADP-ribose, UDP-acetylgalactosamine, UDP-glucuronic acid, UDP-galactose, GDP-fucose, GDP-mannose, UDP-mannose, ADP-mannose, UDP-glucose, TDP-glucose, and ADP-glucose as sugar donors and glycerol, D-2-phosphoglycerate, L-glycerol-3-phosphate, 2,3-diphospho-D-glycerate, and phosphoenolpyruvate
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?
additional information
?
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a single ionizable residue is involved catalysis (pKa = 6.3) that must be deprotonated for full activity. A solvent kinetic isotope effect of 2.0 on kcat is consistent with a proton in flight during the rate-determining step
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-
?
additional information
?
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-
a single ionizable residue is involved catalysis (pKa = 6.3) that must be deprotonated for full activity. A solvent kinetic isotope effect of 2.0 on kcat is consistent with a proton in flight during the rate-determining step
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-
?
additional information
?
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a single ionizable residue is involved catalysis (pKa = 6.3) that must be deprotonated for full activity. A solvent kinetic isotope effect of 2.0 on kcat is consistent with a proton in flight during the rate-determining step
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-
?
additional information
?
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cosubstrate efficiency in decreasing order: UDP-glucose,ADP-glucose and GDP-glucose. No cosubstrate: GDP-mannose
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-
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additional information
?
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-
cosubstrate efficiency in decreasing order: UDP-glucose,ADP-glucose and GDP-glucose. No cosubstrate: GDP-mannose
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-
-
additional information
?
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cosubstrate efficiency in decreasing order: UDP-glucose,ADP-glucose and GDP-glucose. No cosubstrate: GDP-mannose
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-
-
additional information
?
-
the genes for glucosyl-3-phosphoglycerate synthase, GpgS, and glucosyl-3-phosphoglycerate phosphatase, GpgP, the enzymes that lead to the synthesis of glucosylglycerol through the formation of glucosyl-3-phosphoglycerate, are organized in anoperon-like structure with the gene encoding a putative glycosyltransferase, the glucosylglycerate synthase, Ggs, overview, biosynthetic pathway overview
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?
additional information
?
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the genes for glucosyl-3-phosphoglycerate synthase, GpgS, and glucosyl-3-phosphoglycerate phosphatase, GpgP, the enzymes that lead to the synthesis of glucosylglycerol through the formation of glucosyl-3-phosphoglycerate, are organized in anoperon-like structure with the gene encoding a putative glycosyltransferase, the glucosylglycerate synthase, Ggs, overview, biosynthetic pathway overview
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-
?
additional information
?
-
no activity with: ADP-ribose, UDP-acetylgalactosamine, UDP-glucuronic acid, UDP-galactose, GDP-fucose, GDP-mannose, UDP-mannose or ADP-mannose
-
-
?
additional information
?
-
-
no activity with: ADP-ribose, UDP-acetylgalactosamine, UDP-glucuronic acid, UDP-galactose, GDP-fucose, GDP-mannose, UDP-mannose or ADP-mannose
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-
?
additional information
?
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key enzyme of glucosylglycerate synthesis
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-
?
additional information
?
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key enzyme of glucosylglycerate synthesis
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-
?
additional information
?
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enzyme is rather non-specific for glucosyl donors using several glucose diphosphate nucleosides, with ADP-glucose being by far the preferred substrate, followed by UDP-glucose and GDP-glucose. Only 3-PGA can used as glucosyl acceptor leading to the formation of glucosyl 3-phosphoglycerate
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-
?
additional information
?
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-
enzyme is rather non-specific for glucosyl donors using several glucose diphosphate nucleosides, with ADP-glucose being by far the preferred substrate, followed by UDP-glucose and GDP-glucose. Only 3-PGA can used as glucosyl acceptor leading to the formation of glucosyl 3-phosphoglycerate
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-
?
additional information
?
-
enzyme is rather non-specific for glucosyl donors using several glucose diphosphate nucleosides, with ADP-glucose being by far the preferred substrate, followed by UDP-glucose and GDP-glucose. Only 3-PGA can used as glucosyl acceptor leading to the formation of glucosyl 3-phosphoglycerate
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?
additional information
?
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key enzyme of glucosylglycerate synthesis
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?
