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Literature summary for 2.4.1.266 extracted from

  • Empadinhas, N.; Albuquerque, L.; Mendes, V.; Macedo-Ribeiro, S.; da Costa, M.S.
    Identification of the mycobacterial glucosyl-3-phosphoglycerate synthase (2008), FEMS Microbiol. Lett., 280, 195-202.
    View publication on PubMed
Search for more literature for 2.4.1.266

Cloned(Commentary)

Cloned (Comment) Organism
gene gpgS, DNA and amino acid sequence determination and analysis, phylogenetic tree Mycobacterium tuberculosis
gene gpgS, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21 Mycobacterium tuberculosis variant bovis
gene gpgS, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21 Mycolicibacterium smegmatis
overexpressed in Escherichia coli as a C-terminal His-tagged protein Mycobacterium tuberculosis variant bovis
overexpressed in Escherichia coli as a C-terminal His-tagged protein Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information GpgSs exhibits Michaelis-Menten kinetics at 37°C Mycobacterium tuberculosis variant bovis
additional information
-
 additional information GpgSs exhibits Michaelis-Menten kinetics at 37°C Mycolicibacterium smegmatis
0.24
-
 3-phospho-D-glycerate pH 7.5, 37°C Mycobacterium tuberculosis variant bovis
0.24
-
 3-phospho-D-glycerate pH 7.5, 37°C Mycolicibacterium smegmatis
1.28
-
 UDP-glucose pH 7.5, 37°C Mycolicibacterium smegmatis
2.43
-
 UDP-glucose pH 7.5, 37°C Mycobacterium tuberculosis variant bovis
6.08
-
 ADP-glucose pH 7.5, 37°C Mycolicibacterium smegmatis
6.63
-
 ADP-glucose pH 7.5, 37°C Mycobacterium tuberculosis variant bovis
27.7
-
 GDP-glucose pH 7.5, 37°C Mycobacterium tuberculosis variant bovis
32.04
-
 GDP-glucose pH 7.5, 37°C Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ strictly dependent on Mycobacterium tuberculosis
Mg2+ strictly dependent on Mycobacterium tuberculosis variant bovis
Mg2+ strictly dependent on Mycolicibacterium smegmatis
Mg2+ activity is strictly dependent on Mg2+, maximal activation at 20 mM Mycolicibacterium smegmatis
Mg2+ activity is strictly dependent on Mg2+, maximal activation at 20-75 mM Mycobacterium tuberculosis variant bovis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33900
-
 3 * 33900, calculated from sequence Mycolicibacterium smegmatis
34400
-
 3 * 34400, calculated from sequence Mycobacterium tuberculosis variant bovis
101100
-
 gel filtration Mycobacterium tuberculosis variant bovis
106900
-
 gel filtration Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + 3-phospho-D-glycerate Mycobacterium tuberculosis variant bovis involved in biosynthesis of methylated polysaccharides UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
UDP-glucose + 3-phospho-D-glycerate Mycolicibacterium smegmatis involved in biosynthesis of methylated polysaccharides UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
UDP-glucose + 3-phospho-D-glycerate Mycolicibacterium smegmatis ATCC 700084 involved in biosynthesis of methylated polysaccharides UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
 gene gpgS or Rv1208
-
Mycobacterium tuberculosis H37Rv
-
 gene gpgS or Rv1208
-
Mycobacterium tuberculosis variant bovis
-
-
-
Mycobacterium tuberculosis variant bovis
-
 slow-growing strain BCG, gene gpgS
-
Mycolicibacterium smegmatis A0R2E6 fast-growing strain 1102, gene gpgS
-
Mycolicibacterium smegmatis A0R2E6 strain 1102
-
Mycolicibacterium smegmatis ATCC 700084 A0R2E6 strain 1102
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Mycobacterium tuberculosis variant bovis
recombinant enzyme from Escherichia coli Mycolicibacterium smegmatis
recombinant GpgS protein Mycobacterium tuberculosis variant bovis
recombinant GpgS protein Mycolicibacterium smegmatis

Storage Stability

Storage Stability Organism
0°C, purified recombinant enzyme, nearly 100% remaining activity after 1 month Mycobacterium tuberculosis variant bovis
0°C, purified recombinant enzyme, nearly 100% remaining activity after 1 month Mycolicibacterium smegmatis
22°C, room temperature, purified recombinant enzyme, nearly 100% remaining activity after 1 week Mycobacterium tuberculosis variant bovis
22°C, room temperature, purified recombinant enzyme, nearly 100% remaining activity after 1 week Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + D-3-phosphoglycerate
-
 Mycobacterium tuberculosis ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
ADP-alpha-D-glucose + D-3-phosphoglycerate
-
 Mycobacterium tuberculosis variant bovis ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
ADP-alpha-D-glucose + D-3-phosphoglycerate
-
 Mycolicibacterium smegmatis ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
ADP-alpha-D-glucose + D-3-phosphoglycerate
-
 Mycolicibacterium smegmatis ATCC 700084 ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
ADP-alpha-D-glucose + D-3-phosphoglycerate
-
 Mycobacterium tuberculosis H37Rv ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
ADP-glucose + 3-phospho-D-glycerate
-
 Mycobacterium tuberculosis variant bovis ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
ADP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium smegmatis ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
GDP-glucose + 3-phospho-D-glycerate
-
 Mycobacterium tuberculosis variant bovis GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
GDP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium smegmatis GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
GDP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium smegmatis ATCC 700084 GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
UDP-glucose + 3-phospho-D-glycerate
-
 Mycobacterium tuberculosis variant bovis UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
UDP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium smegmatis UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
UDP-glucose + 3-phospho-D-glycerate involved in biosynthesis of methylated polysaccharides Mycobacterium tuberculosis variant bovis UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
UDP-glucose + 3-phospho-D-glycerate involved in biosynthesis of methylated polysaccharides Mycolicibacterium smegmatis UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
UDP-glucose + 3-phospho-D-glycerate
-
 Mycolicibacterium smegmatis ATCC 700084 UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
UDP-glucose + 3-phospho-D-glycerate involved in biosynthesis of methylated polysaccharides Mycolicibacterium smegmatis ATCC 700084 UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
UDP-glucose + D-3-phosphoglycerate best substrates Mycobacterium tuberculosis UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
-
 ?
UDP-glucose + D-3-phosphoglycerate best substrates Mycobacterium tuberculosis variant bovis UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
-
 ?
UDP-glucose + D-3-phosphoglycerate best substrates Mycolicibacterium smegmatis UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
-
 ?
UDP-glucose + D-3-phosphoglycerate best substrates Mycolicibacterium smegmatis ATCC 700084 UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
-
 ?
UDP-glucose + D-3-phosphoglycerate best substrates Mycobacterium tuberculosis H37Rv UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 33900, calculated from sequence Mycolicibacterium smegmatis
trimer 3 * 34400, calculated from sequence Mycobacterium tuberculosis variant bovis

Synonyms

Synonyms Comment Organism
glucosyl-3-phosphoglycerate synthase
-
 Mycobacterium tuberculosis
glucosyl-3-phosphoglycerate synthase
-
 Mycobacterium tuberculosis variant bovis
glucosyl-3-phosphoglycerate synthase
-
 Mycolicibacterium smegmatis
GpgS
-
 Mycobacterium tuberculosis
GpgS
-
 Mycobacterium tuberculosis variant bovis
GpgS
-
 Mycolicibacterium smegmatis
GpgS protein
-
 Mycobacterium tuberculosis variant bovis
GpgS protein
-
 Mycolicibacterium smegmatis
More the enzyme belongs to the retaining family 81 glycosyltransferases Mycobacterium tuberculosis
More the enzyme belongs to the retaining family 81 glycosyltransferases Mycobacterium tuberculosis variant bovis
More the enzyme belongs to the retaining family 81 glycosyltransferases Mycolicibacterium smegmatis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Mycobacterium tuberculosis
45
-
-
 Mycobacterium tuberculosis variant bovis
45
-
-
 Mycolicibacterium smegmatis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 55 no activity below 20°C and above 55°C Mycobacterium tuberculosis
20 55 no activity below 20°C and above 55°C Mycobacterium tuberculosis variant bovis
20 55 no activity below 20°C and above 55°C Mycolicibacterium smegmatis
30 50 30°C: about 40% of maximal activity, 50°C: about 70% of maximal activity Mycolicibacterium smegmatis
35 50 30°C: about 35% of maximal activity, 35°C: about 60% of maximal activity, 50°C: about 90% of maximal activity, 55°C: about 25% of maximal activity Mycobacterium tuberculosis variant bovis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 half-life value for inactivation of GpgS is 12.42 h Mycobacterium tuberculosis variant bovis
37
-
 half-life value for inactivation of GpgS is 7.28 h Mycolicibacterium smegmatis
37
-
 half-life 7.28 h Mycolicibacterium smegmatis
37
-
 half-life: 12.42 h Mycobacterium tuberculosis variant bovis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Mycobacterium tuberculosis
8
-
-
 Mycobacterium tuberculosis variant bovis
8
-
-
 Mycolicibacterium smegmatis
8.5
-
-
 Mycolicibacterium smegmatis

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 50% of maximal activity, pH 9.5: about 75% of maximal activity Mycobacterium tuberculosis variant bovis
6 9.5 pH 6.0: about 55% of maximal activity, pH 9.5: about 60% of maximal activity Mycolicibacterium smegmatis
6 10 activity range Mycobacterium tuberculosis variant bovis
6 10 activity range Mycolicibacterium smegmatis