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Literature summary for 2.4.1.266 extracted from

  • Pereira, P.J.B.; Empadinhas, N.; Albuquerque, L.; Sa-Moura, B.; da Costa, M.S.; Macedo-Ribeiro, S.
    Mycobacterium tuberculosis glucosyl-3-phosphoglycerate synthase: structure of a key enzyme in Methylglucose lipopolysaccharide biosynthesis (2008), PLOS One, 3, e3748.
    View publication on PubMedView publication on EuropePMC
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Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour diffusion method at 20°C, three-dimensional structure of the apoenzyme, as well as of its ternary complex with UDP and 3-phosphoglycerate is determined by X-ray crystallography, to a resolution of 2.5 and 2.7 A, respectively Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + 3-phospho-D-glycerate three-dimensional structures provide a molecular explanation for the enzyme’s preference for UDP-containing donor substrates, as well as for its glucose versus mannose discrimination, and uncover the structural determinants for acceptor substrate selectivity Mycobacterium tuberculosis UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
GpgS
-
 Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function the increased resilience of this pathogen is due, to a great extent, to its complex, polysaccharide-rich, and unusually impermeable cell wall. Enzymes involved in glycosidic bond synthesis represent more than 1% of all ORFs identified from Mycobacterium tuberculosis. One of them is GpgS, a retaining glycosyltransferase with low sequence homology to any other glycosyltransferases of known structure, which has been identified in two species of mycobacteria and shown to be essential for the survival of Mycobacterium tuberculosis Mycobacterium tuberculosis