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Literature summary for 2.4.1.266 extracted from

  • Costa, J.; Empadinhas, N.; Goncalves, L.; Lamosa, P.; Santos, H.; da Costa, M.S.
    Characterization of the biosynthetic pathway of glucosylglycerate in the archaeon Methanococcoides burtonii (2006), J. Bacteriol., 188, 1022-1030.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.266

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Methanococcoides burtonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.54
-
 3-phospho-D-glycerate pH 7.6, 30°C Methanococcoides burtonii
1.6
-
 3-phospho-D-glycerate pH 7.6, 50°C Methanococcoides burtonii
1.75
-
 GDP-glucose pH 7.6, 30°C Methanococcoides burtonii
3.7
-
 GDP-glucose pH 7.6, 50°C Methanococcoides burtonii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ strictly dependent on divalent cations in the following order of efficiency: Mn2+, Co2+, Mg2+. Maximal activity at 1 mM Methanococcoides burtonii
Mg2+ strictly dependent on divalent cations in the following order of efficiency: Mn2+, Co2+, Mg2+ Methanococcoides burtonii
Mn2+ strictly dependent on divalent cations in the following order of efficiency: Mn2+, Co2+, Mg2+. Maximal activity at 1 mM Methanococcoides burtonii
additional information K+, Ca2+, Sr2+, Cu2+, Ba2+, and Zn2+, do not stimulate GpgS activity at any concentration Methanococcoides burtonii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, calculated from sequence Methanococcoides burtonii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-glucose + 3-phospho-D-glycerate Methanococcoides burtonii synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP). An mpgS gene coding for mannosyl-3-phosphoglycerate synthase (MpgS) is absent GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanococcoides burtonii Q12XX4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Methanococcoides burtonii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0116
-
 pH 7.6, 30°C Methanococcoides burtonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-glucose + 3-phospho-D-glycerate
-
 Methanococcoides burtonii GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
GDP-glucose + 3-phospho-D-glycerate synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP). An mpgS gene coding for mannosyl-3-phosphoglycerate synthase (MpgS) is absent Methanococcoides burtonii GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
additional information no activity with: ADP-ribose, UDP-acetylgalactosamine, UDP-glucuronic acid, UDP-galactose, GDP-fucose, GDP-mannose, UDP-mannose, ADP-mannose, UDP-glucose, TDP-glucose, and ADP-glucose as sugar donors and glycerol, D-2-phosphoglycerate, L-glycerol-3-phosphate, 2,3-diphospho-D-glycerate, and phosphoenolpyruvate Methanococcoides burtonii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, calculated from sequence Methanococcoides burtonii

Synonyms

Synonyms Comment Organism
GpgS
-
 Methanococcoides burtonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Methanococcoides burtonii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 50% of maximal activity, 70°C: about 50% of maximal activity Methanococcoides burtonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 half-life: 51.3 h Methanococcoides burtonii
23
-
 half-life: 11.5 h Methanococcoides burtonii
50
-
 half-life: 13.8 h Methanococcoides burtonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Methanococcoides burtonii

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 pH 6.5: about 55% of maximal activity, pH 9.5: about 70% of maximal activity Methanococcoides burtonii