EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    3.2.1.91 | -999 |
- |
irreversible thermal unfolding kinetics of recombinant enzyme domains, overview |
664959 |
| 3.2.1.91 | -999 |
- |
the cellulose-binding domain of Cel7A is shown to be thermostabilizing the whole protein by dynamic light scattering. Alone, it displays a melting point 14-15°C higher than that of the catalytic domain and 6-7°C higher than that of the whole protein |
748262 |
| 3.2.1.91 | -999 |
- |
thermal unfolding kinetics of wild-type and mutant enzymes, overview |
666803 |
| 3.2.1.91 | 30 |
60 |
the enzyme is stable for 1 h up to 60°C |
752111 |
| 3.2.1.91 | 30 |
60 |
the enzyme is stable up to 50°C, but is dramatically inactivated at 60°C (8% activity remaining after 1 h) |
745229 |
| 3.2.1.91 | 35 |
55 |
- |
742765 |
| 3.2.1.91 | 40 |
- |
3 hours |
393734 |
| 3.2.1.91 | 40 |
- |
purified recombinant enzymes, 30 min, good stability up to 40°C |
732493 |
| 3.2.1.91 | 40 |
70 |
the enzyme maintains about 90% activity when incubated at 60°C for 24 h. About 90% activity is maintained after 8 h of incubation at 70°C. At temperatures over 80°C, the enzyme activity sharply decreases depending on the incubation time. The enzyme shows half-life values of 552 h, 144 h, 96 h, and 42 h at 40°C, 50°C, 60°C, and 70°C, respectively |
715879 |
| 3.2.1.91 | 40 |
90 |
after 1 h at 40, 50, 60, 70, 80, and 90°C, the wild type enzyme retains about 100%, 99%, 93%, 70%, 47%, and 21% activity, respectively. The half-life at 90°C is 25 min |
750797 |
