Information on EC 3.6.1.23 - dUTP diphosphatase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.6.1.23
-
RECOMMENDED NAME
GeneOntology No.
dUTP diphosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dUTP + H2O = dUMP + diphosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphorous acid anhydride hydrolysis
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
pyrimidine deoxyribonucleotides de novo biosynthesis I
-
-
pyrimidine deoxyribonucleotides de novo biosynthesis II
-
-
pyrimidine deoxyribonucleotides de novo biosynthesis III
-
-
pyrimidine deoxyribonucleotides dephosphorylation
-
-
pyrimidine metabolism
-
-
Pyrimidine metabolism
-
-
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli)
-
-
SYSTEMATIC NAME
IUBMB Comments
dUTP nucleotidohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
37289-34-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
B-PG9
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene yosS
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Escherichia coli overproducing
overproducing strain
-
-
Manually annotated by BRENDA team
R2
-
-
Manually annotated by BRENDA team
human herpes simplex virus type 2
-
-
-
Manually annotated by BRENDA team
human herpesvirus 6A GS
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
subsp. mycoides
-
-
Manually annotated by BRENDA team
no activity in caprine lentivirus genotype E
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
RGV, an iridovirus pathogenic in the pig frog
-
-
Manually annotated by BRENDA team
WSSV, ORF wsv112 or gene wdut
-
-
Manually annotated by BRENDA team
gene DUT
-
-
Manually annotated by BRENDA team
Sulfolobus islandicus rod-shaped virus SIRV
Uniprot
Manually annotated by BRENDA team
strain NA1
SwissProt
Manually annotated by BRENDA team
strain 427, gene DUT with T49 and E248
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
DURP gene, several different virus of the alpha-, beta-, and gammaherpesviridae, e.g. HSV-1, HHV-8, or HCMV, overview
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2',3'-dideoxyUTP + H2O
2',3'-dideoxy-UMP + diphosphate
show the reaction diagram
-
76% of the activity with dUTP
-
-
?
2'-deoxy-5-fluoro-UTP + H2O
2'-deoxy-5-fluoro-UMP + diphosphate
show the reaction diagram
6-azaUTP + H2O
6-azaUMP + diphosphate
show the reaction diagram
-
39% of the activity with dUTP
-
-
?
alpha,beta-imido-dUTP + H2O
dUMP + amino-diphosphate
show the reaction diagram
-
-
-
?
araUTP + H2O
araUMP + diphosphate
show the reaction diagram
-
90% of the activity with dUTP
-
-
?
beta-L-2'-dUTP + H2O
beta-L-2'-dUMP + diphosphate
show the reaction diagram
dATP + H2O
dAMP + diphosphate
show the reaction diagram
dCTP + H2O
dCMP + diphosphate
show the reaction diagram
dGTP + H2O
dGMP + diphosphate
show the reaction diagram
dITP + H2O
dIMP + diphosphate
show the reaction diagram
shows also significant activity against dITP
-
-
?
dTTP + H2O
dTMP + diphosphate
show the reaction diagram
dUDP + H2O
deoxyuridine + diphosphate
show the reaction diagram
dUDP + H2O
dUMP + phosphate
show the reaction diagram
dUTP + H2O
?
show the reaction diagram
dUTP + H2O
dUMP + diphosphate
show the reaction diagram
dUTP + H2O
UMP + diphosphate
show the reaction diagram
dUTPalphaS + H2O
dUMP + thiodiphosphate
show the reaction diagram
-
-
-
-
?
UTP + H2O
UMP + diphosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dCTP + H2O
dCMP + diphosphate
show the reaction diagram
A7J1X3
-
-
-
?
dUTP + H2O
?
show the reaction diagram
dUTP + H2O
dUMP + diphosphate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NaCl
-
maximal activity at 0.1 M
VO2+
-
results a higher activity than Mg2+ or Mn2+
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(E)-3-(2,2-difluoroethoxy)-N-(7-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-2-methylhept-5-en-2-yl)benzenesulfonamide
-
-
(E)-3-(cyclopentyloxy)-N-(7-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-2-methylhept-5-en-2-yl)benzenesulfonamide
-
-
(E)-3-(cyclopropylmethoxy)-N-(7-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-2-methylhept-5-en-2-yl)benzenesulfonamide
-
-
(E)-N-(7-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-2-methylhept-5-en-2-yl)-3-methoxybenzenesulfonamide
-
-
(E)-N-(7-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-2-methylhept-5-en-2-yl)benzenesulfonamide
-
-
(R)-1-(3-(2-(hydroxydiphenylmethyl)-pyrrolidin-1-ylsulfonyl)propyl)pyrimidine-2,4(1H,3H)-dione
-
-
(R)-1-(4-(2-(hydroxydiphenylmethyl)-pyrrolidin-1-yl)-4-oxobutyl)pyrimidine-2,4(1H,3H)-dione
-
-
(R)-N-(1-(3-(2,2-difluoroethoxy)phenyl)ethyl)-4-((2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)benzenesulfonamide
-
-
(R)-N-(1-(3-(cyclopentyloxy)phenyl)ethyl)-4-((2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)benzenesulfonamide
-
-
(R)-N-(1-(3-(cyclopropylmethoxy)phenyl)ethyl)-4-((2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)-benzenesulfonamide
-
-
(R,E)-N-(1-(3-(2,2-difluoroethoxy)phenyl)ethyl)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)pent-3-ene-1-sulfonamide
-
-
(R,E)-N-(1-(3-(cyclopentyloxy)phenyl)ethyl)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)pent-3-ene-1-sulfonamide
-
-
(R,E)-N-(1-(3-(cyclopropylmethoxy)phenyl)ethyl)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)pent-3-ene-1-sulfonamide
-
-
(S)-1-(4-(2-(hydroxydiphenylmethyl)-pyrrolidin-1-yl)-4-oxobutyl)pyrimidine-2,4(1H,3H)-dione
-
-
1,10-phenanthroline
1-(2-amido-4-trityloxybutyl)uracil
-
-
1-(2-dimethylamido-4-trityloxybutyl)uracil
-
-
1-(2-morpholinamido-4-trityloxybutyl)uracil
-
-
1-(3-tert-butyldimethylsilyloxypropyl)uracil
1-(3-triphenylsilyloxypropyl)uracil
1-(3-tritylaminopropyl)uracil
1-(3-trityloxypropyl)uracil
1-(3-[[(2S)-2-[hydroxy[bis(2-methoxyphenyl)]methyl]pyrrolidin-1-yl]sulfonyl]propyl)pyrimidine-2,4(1H,3H)-dione
-
-
1-(3-[[(2S)-2-[hydroxy[bis(3-methoxyphenyl)]methyl]pyrrolidin-1-yl]sulfonyl]propyl)pyrimidine-2,4(1H,3H)-dione
-
-
1-(4-[(2S)-2-[bis(3-chlorophenyl)(hydroxy)methyl]pyrrolidin-1-yl]-4-oxobutyl)pyrimidine-2,4(1H,3H)-dione
-
-
1-(4-[(2S)-2-[bis(3-fluorophenyl)(hydroxy)methyl]pyrrolidin-1-yl]-4-oxobutyl)pyrimidine-2,4(1H,3H)-dione
-
-
1-(4-[(2S)-2-[bis(4-chlorophenyl)(hydroxy)methyl]pyrrolidin-1-yl]-4-oxobutyl)pyrimidine-2,4(1H,3H)-dione
-
-
1-(4-[(2S)-2-[bis(4-fluorophenyl)(hydroxy)methyl]pyrrolidin-1-yl]-4-oxobutyl)pyrimidine-2,4(1H,3H)-dione
-
-
1-(5-triphenylsilyloxypentyl)uracil
1-(5-tritylaminopentyl)uracil
1-(5-trityloxypentyl)uracil
1-[(1E,8E)-5-([[(E)-(3,4-dihydroxy-5-methoxybenzylidene)amino]oxy]methyl)-1-(3,4-dihydroxy-5-methoxyphenyl)-3,7-dioxa-2,8-diazadeca-1,8-dien-10-yl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[(1Z,8E)-5-([[(E)-(3,4-dihydroxy-5-methoxybenzylidene)amino]oxy]methyl)-1-(3,4-dihydroxy-5-methoxyphenyl)-3,7-dioxa-2,8-diazadeca-1,8-dien-10-yl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[(2E)-4-(trityloxy)but-2-en-1-yl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[(2R,5S)-5-([[tert-butyl(diphenyl)silyl]oxy]methyl)-2,5-dihydrofuran-2-yl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[(2R,5S)-5-[(trityloxy)methyl]-2,5-dihydrofuran-2-yl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[(E)-4-trityloxy-2-butenyl]uracil
1-[(Z)-4-trityloxy-2-butenyl]uracil
1-[2-(acetamido)-4-(tert-butyldiphenylsilyloxy)butyl]uracil
-
-
1-[2-(acetamido)-4-(triphenylmethyl)aminobutyl]uracil
-
-
1-[2-(azidomethyl)-4-(trityloxy)butyl]uracil
1-[2-(carbobenzoxymethyl)-4-(triphenylmethyl)aminobutyl]uracil
-
-
1-[2-(carboethoxyethylamido)-4-trityloxybutyl]uracil
-
-
1-[2-(carboxy)-4-(tert-butyldiphenylsilyloxy)butyl]uracil
-
-
1-[2-(carboxy)-4-(triphenylmethyl)aminobutyl]uracil
-
-
1-[2-(carboxyethylamido)-4-trityloxybutyl]uracil
-
-
1-[2-(diethylamido)-4-(tert-butyldiphenylsilyloxy)butyl]uracil
-
-
1-[2-(ethylamido)-4-(tert-butyldiphenylsilyloxy) butyl]uracil
-
-
1-[2-(hydroxyethylamido)-4-trityloxybutyl]uracil
-
-
1-[2-(hydroxymethyl)-4-(trityloxy)butyl]uracil
1-[2-(methoxyethylamido)-4-trityloxybutyl]uracil
-
-
1-[2-(methylamido)-4-(tert-butyldiphenylsilyloxy)butyl]uracil
-
-
1-[2-(N,N-dimethylaminoethylamido)-4-trityloxybutyl]uracil
-
-
1-[2-(N-benzyloxycarbonylpiperazinamido)-4-(tert-butyldiphenylsilyloxy)butyl]uracil
-
-
1-[2-(N-methylpiperazine)amido-4-trityloxybutyl]uracil
-
-
1-[2-(tert-butyldiphenylsilyloxyethylamido)-4-trityloxybutyl]uracil
-
-
1-[2-(trityloxy)ethoxymethyl]uracil
1-[3-(4-benzylpiperidin-1-yl)-3-oxopropyl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[3-(benzhydrylaminocarbonyl)propyl]uracil
-
1429fold selectivity over human enzyme
1-[3-(tritylamino)propyl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[3-(tritylaminocarbonyl)propyl]uracil
-
4545fold selectivity over human enzyme
1-[3-([(2R)-2-[hydroxy(diphenyl)methyl]pyrrolidin-1-yl]sulfonyl)propyl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[3-([(2S)-2-[bis(3-fluorophenyl)(hydroxy)methyl]pyrrolidin-1-yl]sulfonyl)propyl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[3-([(2S)-2-[bis(4-fluorophenyl)(hydroxy)methyl]pyrrolidin-1-yl]sulfonyl)propyl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[3-[(2S)-2-(diphenylmethyl)pyrrolidin-1-yl]-3-oxopropyl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[4-(tert-butoxycarbonylamino)-3-(trityloxymethyl)butyl]uracil
1-[4-acetoxy-3-(tritylaminomethyl)butyl]uracil
1-[4-hydroxy-2-(trityloxymethyl)butyl]uracil
1-[4-hydroxy-3-(tritylaminomethyl)butyl]uracil
1-[4-hydroxy-3-[(tritylamino)methyl]butyl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[4-[(2S)-2-(diphenylmethyl)pyrrolidin-1-yl]-4-oxobutyl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[4-[(2S)-2-[bis[2-(benzyloxy)phenyl](hydroxy)methyl]pyrrolidin-1-yl]-4-oxobutyl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[4-[(2S)-2-[bis[3-(benzyloxy)phenyl](hydroxy)methyl]pyrrolidin-1-yl]-4-oxobutyl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[4-[(2S)-2-[bis[4-(benzyloxy)phenyl](hydroxy)methyl]pyrrolidin-1-yl]-4-oxobutyl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[4-[(2S)-2-[hydroxy[di(thiophen-3-yl)]methyl]pyrrolidin-1-yl]-4-oxobutyl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[6-(tritylamino)hexyl]pyrimidine-2,4(1H,3H)-dione
-
-
1-[N-(2-triphenylmethylaminoethyl)-acetamide]uracil
-
-
1-[N-(3-triphenylmethylaminopropyl)-acetamide]uracil
-
-
1-[N-(4-triphenylmethylaminobutyl)-acetamide]uracil
-
-
1-[N-(triphenylmethyl) diaminodi(n-propyl) acetamide]uracil
-
-
1-[N-(triphenylmethylaminoethyl)-N-(aminoethyl) acetamide]uracil
-
-
1-[N-hydroxyethyl-N-(triphenylmethylamino) ethyl-acetamide]uracil
-
-
1-[[2-(trityloxy)ethoxy]methyl]pyrimidine-2,4(1H,3H)-dione
2',3',5'-trideoxy-3'-fluoro-5'-(tritylamino)uridine
-
activity against the parasites with IC50: 0.0053 mM
2',3'-didehydro-2',3'-dideoxyuridine 5'-diphenyl phosphate
2',3'-dideoxy-3'-fluoro-5'-O-trityluridine
2',5'-dideoxy-5'-(tritylamino)uridine
2',5'-dideoxyuridine 5'-N-diphenylphosphoramidate
2'-deoxy-5'-O-(triphenylsilyl)uridine
-
activity against the parasites with IC50: 0.0011 mM
2'-deoxy-5'-O-trityluridine
2'-deoxy-5'-O-[tris(1-methylethoxy)silyl]uridine
-
activity against the parasites with IC50: 0.013 mM
2'-deoxy-5'-phenylmethylaminouracil
-
-
2'-deoxy-5'-triphenylmethanyluracil
-
-
2'-deoxyuracil 5'-diphenyl phosphate
-
-
2'-deoxyuridine
-
competitive inhibition
2'-deoxyuridine 5'-(alpha,beta-imido)triphosphate
3'-azido-2',3'-dideoxy-UTP
-
competitive inhibitor
3'-deoxy-5'-O-(hydroxy{[hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl)uridine
3'-deoxy-5'-O-[hydroxy(phosphonoamino)phosphoryl]uridine
-
competitive inhibition
3'-O-tert-butyldimethylsilyl-2',5'-dideoxyuridine 5'-N-diphenylphosphoramidate
3'-O-tert-butyldimethylsilyl-5'-O-sulfamoyl-2'-deoxyuridine
3-(2,2-difluoroethoxy)-N-(2-(4-((2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)phenyl)propan-2-yl)-benzenesulfonamide
-
-
3-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2,4,4-trimethylpentan-2-yl)propanamide
-
-
3-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2-methylbutan-2-yl)propanamide
-
-
3-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2-phenylethyl)propanamide
-
-
3-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(3-ethylhexan-3-yl)propanamide
-
-
3-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(3-phenylpropyl)propanamide
-
-
3-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(diphenylmethyl)propanamide
-
-
3-(cyclopentyloxy)-N-(2-(4-((2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)phenyl)propan-2-yl)-benzenesulfonamide
-
-
3-(cyclopropylmethoxy)-N-(2-(4-((2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)phenyl)propan-2-yl)-benzenesulfonamide
-
-
3-(cyclopropylmethoxy)-N-[5-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methoxy]-2-methylpentan-2-yl]benzenesulfonamide
-
-
3-[5-(trityloxy)tetrahydrofuran-2-yl]pyridine-2,6(1H,3H)-dione
-
-
4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(1-hydroxy-2-methyl-1,1-diphenylpropan-2-yl)butanamide
-
-
4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(1-hydroxy-2-methylpropan-2-yl)butanamide
-
-
4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2,2-diphenylethyl)-N-methylbutanamide
-
-
4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2,2-diphenylethyl)butanamide
-
-
4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2,4,4-trimethylpentan-2-yl)butanamide
-
-
4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2-hydroxy-2,2-diphenylethyl)-N-methylbutanamide
-
-
4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2-methyl-1-phenylpropan-2-yl)butanamide
-
-
4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2-methyl-4-phenylbutan-2-yl)butanamide
-
-
4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(2-phenylpropan-2-yl)butanamide
-
-
4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(3-ethylpentan-3-yl)butanamide
-
-
4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-(diphenylmethyl)butanamide
-
-
4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-phenylbutanamide
-
-
4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-N-[2-methyl-1-(naphthalen-2-yl)propan-2-yl]butanamide
-
-
5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-2',3'-dideoxyuracil
-
-
5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-2'-deoxyuracil
-
-
5'-(2,2-dimethyl-1,1-diphenylpropan-1-yl)-3'-methyl-2',3'-dideoxyuracil
-
-
5'-(2,3,3-trimethylbutan-2-yl)-3'-methyl-2',3'-dideoxyuracil
-
-
5'-(2,4-dimethyl-3-(1-methylethyl)pentan-3-yl)-2'-deoxyuracil
-
-
5'-(P3-benzyl-alpha,beta-methylene)-2'deoxyuridine
-
-
5'-adamantoyl-3'-methyl-2',3'-dideoxyuracil
-
-
5'-hexadecanoyl-3'-methyl-2',3'-dideoxyuracil
-
-
5'-O-(1-adamantoyl)-2',3'-didehydro-2',3'-dideoxyuridine
5'-O-(4,4'-dimethoxytrityl)-2'-deoxyuridine
-
-
5'-O-sulfamoylcarbamoyl-2',3'-didehydro-2',3'-dideoxyuridine
5'-O-triphenylsilyl-2',3'-didehydro-2',3'-dideoxyuridine
5'-O-triphenylsilyl-2',3'-dideoxy-3'-fluorouridine
5'-O-triphenylsilyl-2'-deoxyuridine
5'-pentanoylamino-2',5'-dideoxyuridine
5'-tert-butyldiphenylsilyloxy-2',3'-dideoxy-3'-fluorouridine
5'-tritylamino-2',5'-dideoxyuridine
5'-tritylamino-3'-fluoro-2',3',5'-trideoxyuridine
5-Aminouracil
-
5 mM, 50% loss of activity
6-aminouracil
-
5 mM, 60% loss of activity
8-azido-ATP
-
competitive
alpha,beta-imido-dUDP
alpha,beta-imido-dUTP
alpha-beta-imido-dUTP
-
competitive, strong inhibition
bacteriophage PBS2-induced inhibitor
-
seems to form a high MW complex with the Bacillus subtilis dUTPase, the lower MW enzyme form of dUTPase is not inactivated by the protease inhibitor
-
beta-hydroxyisovalerylshikonin
-
an ATP-noncompetitive inhibitor of protein tyrosine kinases, induces apoptosis in human lung cancer DMS114 cells through reduction of dUTP nucleotidohydrolase activity, acts synergistically with 5-fluorouracil, overview
bovine serum albumin
38% inhibition at 0.01%
-
diphosphate
-
-
dUMP alphaS
-
-
dUTPalphaS
-
-
-
fluorodeoxy-UMP
guanidine hydrochloride
-
kinetics of GuHCl-induced denaturation of the two dUTPase isozymes at pH 7.5, 4 C and 1.5-4 M
Hydroxymercuribenzoate
-
5 mM, complete inhibition
-
KCl
-
0.4 mM, 29% loss of activity
Mg2+
-
complete inhibition by extensive dialysis against 0.0125 M MgCl2
N,N-dibenzyl-3-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)propanamide
-
-
N-(2-(4-((2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)-phenyl)propan-2-yl)-3-methoxybenzenesulfonamide
-
-
N-(2-(4-((2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)-phenyl)propan-2-yl)benzenesulfonamide
-
-
N-benzyl-4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
-
-
N-tert-butyl-4-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)butanamide
-
-
N-[(1R)-1-[3-(cyclopropylmethoxy)-4-fluorophenyl]ethyl]-3-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methoxy]propane-1-sulfonamide
-
-
N-[(1R)-1-[3-(cyclopropylmethoxy)phenyl]ethyl]-3-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methoxy]propane-1-sulfonamide
-
-
N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]-D-alanyl-L-tyrosine
-
-
N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]-L-alanyl-L-aspartic acid
-
-
N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]-L-alanyl-L-glutamic acid
-
-
N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]-L-alanyl-L-glutamine
-
-
N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]-L-alanyl-L-lysine
-
-
N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]-L-alanyl-L-threonine
-
-
N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]-L-alanylglycine
-
-
N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]glycyl-L-lysine
-
-
N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]glycyl-L-threonine
-
-
N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]glycyl-L-tyrosine
-
-
N-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]glycylglycine
-
-
N-[2-[3-(cyclopropylmethoxy)phenyl]propan-2-yl]-3-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methoxy]propane-1-sulfonamide
-
-
N-[3-(tert-butyl diphenyl silanyloxy)-propyl]-2-uracil acetamide
-
-
N-[4-(tert-butyl diphenyl silanyloxy)-butyl]-2-uracil acetamide
-
-
N-[5-[(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methoxy]-2-methylpentan-2-yl]benzenesulfonamide
-
-
NEM
-
12 mM, 14% loss of activity
siRNAdUT3
a 21 base-pair double-stranded RNA molecule targeted against the motif 3 of the enzyme, time- and dose-dependent decrease in the dUTPase activity in transfected cells
-
thymidine 5'-(alpha,beta-imido)triphosphate
-
-
thymidine triphosphate
binding of thymidine triphosphate leads to disordered C-terminal arranged as a lid covering the active site, and the enzyme adapts an inactive conformation as a result of structural changes in the active site, mechanism, overview
Uracil
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
50-500 mM, 25% stimulation
bis(acetylacetonato)oxovanadium(IV)
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00173 - 0.0061
2'-deoxy-5-fluoro-UTP
0.438 - 0.523
beta-L-2'-dUTP
1.13
dATP
-
at 25C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0
0.465 - 2.3
dCTP
1.96
dGTP
-
at 25C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0
0.0447
dITP
wild type enzyme, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30C
0.26 - 0.761
dTTP
0.0062 - 0.17
dUDP
0.0001 - 0.5
dUTP
0.0027
dUTP-Co2+
-
pH 7.0, 25C, recombinant enzyme
0.0019
dUTP-Mg2+
-
pH 7.0, 25C, recombinant enzyme
0.0137
dUTP-Mn2+
-
pH 7.0, 25C, recombinant enzyme
0.0002
dUTPalphaS
-
-
-
0.035 - 1
UTP
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.4
dATP
Leishmania major
-
pH 8, 25C
0.7 - 13
dCTP
6.4
dGTP
Leishmania major
-
pH 8, 25C
1.3
dITP
Saccharomyces cerevisiae
P33317
wild type enzyme, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30C
0.1 - 8.6
dTTP
65
dUDP
Leishmania major
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