Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a potential drug target against malaria | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
expression of the recombinant enzyme | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-deoxyuridine | competitive inhibition | Plasmodium falciparum | |
alpha,beta-imido-dUTP | competitive inhibition | Plasmodium falciparum | |
dUDP | competitive inhibition | Plasmodium falciparum | |
dUMP | product, competitive inhibition | Plasmodium falciparum | |
dUTP | - |
Plasmodium falciparum | |
EDTA | causes a 105fold decrease and a 12fold increase of the kcat and Km values for dUTP hydrolysis, respectively, compared to the dUTP-Mg2+ complex | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten and ligand binding kinetics, overview. Thermodynamic parameters for the interaction between Plasmodium falciparum dUTPase and deoxyuridine derivatives at 25.2 °C and pH 7.0 | Plasmodium falciparum | |
0.0019 | - |
dUTP | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
0.0019 | - |
dUTP-Mg2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
0.0027 | - |
dUTP-Co2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
0.0137 | - |
dUTP-Mn2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can substitute the physiological cofactor Mg2+, however the kcat is significantly reduced compared to dUTP-Mg2+ | Plasmodium falciparum | |
Mg2+ | dependent on, the energetic barrier of the reaction is 4fold higher when Mg2+ is depleted | Plasmodium falciparum | |
Mn2+ | can substitute the physiological cofactor Mg2+, however the kcat is significantly reduced compared to dUTP-Mg2+ | Plasmodium falciparum | |
NaCl | maximal activity at 0.1 M | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | Plasmodium falciparum | - |
dUMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme by gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | - |
Plasmodium falciparum | dUMP + diphosphate | - |
? | |
dUTP + H2O | dUTPase catalyzes the hydrolysis of the alpha,beta-diphosphate bond of dUTP | Plasmodium falciparum | dUMP + diphosphate | - |
? | |
dUTP-Co2+ + H2O | - |
Plasmodium falciparum | dUMP-Co2+ + diphosphate | - |
? | |
dUTP-Mg2+ + H2O | - |
Plasmodium falciparum | dUMP-Mg2+ + diphosphate | - |
? | |
dUTP-Mn2+ + H2O | - |
Plasmodium falciparum | dUMP-Mn2+ + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | - |
Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
deoxyuridine 5'-triphosphate nucleotidohydrolase | - |
Plasmodium falciparum |
dUTPase | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
dUTP | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
4.1 | - |
dUTP-Co2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
5.3 | - |
dUTP-Mn2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
13.3 | - |
dUTP-Mg2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0718 | - |
dUTP-Mn2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
0.092 | - |
dUTP-Co2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
0.0955 | - |
dUTP-Mg2+ | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
0.099 | - |
dUMP | pH 7.0, 25°C | Plasmodium falciparum | |
0.115 | - |
dUTP | pH 7.0, 25°C, recombinant enzyme | Plasmodium falciparum | |
0.121 | - |
dUTP | pH 7.0, 25°C, recombinant enzyme, without NaCl, presence of EDTA | Plasmodium falciparum |