Literature summary for 3.6.1.23 extracted from

Li, G.L.; Wang, J.; Li, L.F.; Su, X.D.
Crystallization and preliminary X-ray analysis of three dUTPases from Gram-positive bacteria (2009), Acta Crystallogr. Sect. F, 65, 339-342.

Cloned(Commentary)

Cloned (Comment)	Organism
gene dut, DNA and amino acid sequence determination and analysis, high level overexpression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)	Streptococcus mutans
genes yncF and yosS, DNA and amino acid sequence determination and analysis, high level overexpression of N-terminally His6-tagged enzymes in Escherichia coli strain BL21(DE3)	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, sitting drop vapour diffusion method, 15 mg/ml protein in 20 mM Tris-HCl, and 150 mM NaCl, pH 7.5, with 0.2 M MgCl2, 25% w/v PEG 3350, 0.1 M Tris-HCl, pH 8.5, X-ray diffraction structure determination and analysis at 1.3 A resolution	Streptococcus mutans
purified recombinant selenomethionine-labelled YosS and YncF, hanging drop vapour diffusion method, for YosS: 30 mg/ml protein in 20 mM Tris-HCl, and 150 mM NaCl, pH 7.5, with 2.0 M ammonium sulfate, 0.2 M potassium/sodium tartrate, 0.1 M trisodium citrate, pH 5.6, in 3 days, for YncF: 20 mg/ml protein in 20 mM Tris-HCl, and 150 mM NaCl, pH 7.5, with 0.2 M trimethylamine N-oxide dehydrate, 15% w/v PEG MME 2000, 0.1 M Tris-HCl, pH 9.0, in two weeks, X-ray diffraction structure determination and analysis at 2.3 A and 1.7 A resolution, respectively	Bacillus subtilis

Crystallization (Comment)

Organism

purified recombinant enzyme, sitting drop vapour diffusion method, 15 mg/ml protein in 20 mM Tris-HCl, and 150 mM NaCl, pH 7.5, with 0.2 M MgCl2, 25% w/v PEG 3350, 0.1 M Tris-HCl, pH 8.5, X-ray diffraction structure determination and analysis at 1.3 A resolution

Streptococcus mutans

purified recombinant selenomethionine-labelled YosS and YncF, hanging drop vapour diffusion method, for YosS: 30 mg/ml protein in 20 mM Tris-HCl, and 150 mM NaCl, pH 7.5, with 2.0 M ammonium sulfate, 0.2 M potassium/sodium tartrate, 0.1 M trisodium citrate, pH 5.6, in 3 days, for YncF: 20 mg/ml protein in 20 mM Tris-HCl, and 150 mM NaCl, pH 7.5, with 0.2 M trimethylamine N-oxide dehydrate, 15% w/v PEG MME 2000, 0.1 M Tris-HCl, pH 9.0, in two weeks, X-ray diffraction structure determination and analysis at 2.3 A and 1.7 A resolution, respectively

Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
16100	-	3 * 16100, SDS-PAGE	Streptococcus mutans

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	genes yncF and yosS	-
Streptococcus mutans	-	gene DUT	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and gel filtration	Bacillus subtilis
recombinant N-terminally His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity and gel filtration	Streptococcus mutans

Subunits

Subunits	Comment	Organism
homotrimer	-	Bacillus subtilis
homotrimer	3 * 16100, SDS-PAGE	Streptococcus mutans

Synonyms

Synonyms	Comment	Organism
dUTPase	-	Bacillus subtilis
dUTPase	-	Streptococcus mutans
YncF	-	Bacillus subtilis
YosS	-	Bacillus subtilis