Information on EC 3.5.4.1 - cytosine deaminase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.5.4.1
-
RECOMMENDED NAME
GeneOntology No.
cytosine deaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cytosine + H2O = uracil + NH3
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
pyrimidine nucleobases salvage II
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pyrimidine ribonucleosides salvage III
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pyrimidine metabolism
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Pyrimidine metabolism
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Arginine and proline metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
cytosine aminohydrolase
Also acts on 5-methylcytosine.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-05-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ssp. denitrificans J9
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-
Manually annotated by BRENDA team
Achromobacter liquidum
IFO 3084
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-
Manually annotated by BRENDA team
Achromobacter polymorph
AKU 0122
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Manually annotated by BRENDA team
Achromobacter polymorph AKU 0122
AKU 0122
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
AKU 0300
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-
Manually annotated by BRENDA team
IAM 1526
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Manually annotated by BRENDA team
AKU 0101
-
-
Manually annotated by BRENDA team
IAM 0101
-
-
Manually annotated by BRENDA team
Alcaligenes faecalis type S
type S
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-
Manually annotated by BRENDA team
Alcaligenes viscolactis
IAM 1517
-
-
Manually annotated by BRENDA team
Alcaligenes viscolactis IAM 1517
IAM 1517
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-
Manually annotated by BRENDA team
JH-13
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Manually annotated by BRENDA team
IFO5840
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
IFO 3329
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Manually annotated by BRENDA team
IFO 3026
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Manually annotated by BRENDA team
Candida dubliniensis isolates SA113, SA109, Eg202, Eg204 and p7276
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Enterobacter aerogenes type S
type S
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Manually annotated by BRENDA team
IAM 1221
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Manually annotated by BRENDA team
Enterobacter cloacae type S
type S
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Manually annotated by BRENDA team
Escherichia coli GIA39(DE3)
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SwissProt
Manually annotated by BRENDA team
strain K12
SwissProt
Manually annotated by BRENDA team
Escherichia coli K12 AKU 0005
strain K12 AKU 0005
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Manually annotated by BRENDA team
Flavobacterium filamentosum
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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1180923 A+, 1234243 A+, 1241781 A+, 1244667 A+, 1244777 A+, 1245036 A+, 1245171 A+, 1245369 A+, 1245423 A+, 1245558 A+, 1255648 A+, 1263457 A+, 1276034 A+, 1278977 A+, 1281439 A+, 1305851 A+, 1346847 A+, 1507347 A+, 1562627 A+, 1563093 A+, 1563401 A+, 1564525 A+, 1597447 A+, 1597509 A+, 1597841 A+, 1616398 A+, 1620311 A+, 1620314 A+, 1620375 A+, 1620421 A+, 1620461 A+, 1620469 A+, 1620487 A+, 1620616 A+, 1631072 A+, 1631073 A+, 1631074 A+, 1631075 A+, 1631076 A+, 1631077 A+, 1631083 A+, 1631088 A+, 1631098 A+, 1631099 A+, 1631100 A+, 1631101 A+, 1631107 A+, 1631114 A+, 1631121 A+, 1631124 A+, 1631125 A+, 1631126 A+, 1631127 A+, 1631129 A+, 1631135 A+, 1631137 A+, 1631139 A+, 1631140 A+, 1631143 A+, 1631150 A+, 1631159 A+, 1631162 A+, 1631166 A+, 1631181 A+, 1631182 A+, 1631189 A+, 1631192 A+, 1631195 A+, 1631201 A+, 1631207 A+, 1631208 A+, 1631209 A+, 1631215 A+, 1631219 A+, 1631222 A+, 1631224 A+, 1631226 A+, 1631229 A+, 1631230 A+, 1631231 A+, 1631238 A+, 1631241 A+, 1631243 A+, 1631244 A+, 1631252 A+, 1631255 A+, 1631256 A+, 1631262 A+, 1631268 A+, 1631269 A+, 1631272 A+, 1631273 A+, 1631276 A+, 1631277 A+, 1631279 A+, 1631280 A+, 1631286 A+, 1631288 A+, 1631290 A+, 1631293 A+, 1631295 A+, 1631296 A+, 1631303 A+, 1631305 A+, 1631306 A+, 1631312 A+, 1631313 A+, 1631316 A+, 1631319 A+, 1631320 A+, 1631323 A+, 1631327 A+, 1631328 A+, 1631329 A+, 1631334 A+, 1631336 A+, 1631337 A+, 1631340 A+, 1631343 A+, 1631349 A+, 1631353 A+, 1631354 A+, 1631355 A+, 1631358 A+, 1631360 A+, 1631364 A+, 1631365 A+, 1631366 A+, 1631373 A+, 1631376 A+, 1631381 A+, 1631383 A+, 1631384 A+, 1631385 A+, 1631389 A+, 1631393 A+, 1631396 A+, 1631400 A+, 1631408 A+, 1631410 A+, 1631411 A+, 1631414 A+, 1631419 A+, 1631421 A+, 1631422 A+, 1631425 A+, 1631428 A+, 1631430 A+, 1631431 A+, 1631433 A+, 1631437 A+, 2210950 A+, 2210956 A+, 2212744 A+, 2212746 A+, 2212747 A+, 2212748 A+, 2212749 A+, 2212750 A+, 2212754 A+, 2212755 A+, 2212759 A+, 2212761 A+, 2212762 A+, 2212763 A+, 2212766 A+, 2212769 A+, 2212776 A+, 2212780 A+, 2212787 A+, 2212788 A+, 2212790 A+, 2212791 A+, 2212792 A+, 2212793 A+, 2212794 A+, 2212796 A+, 2212797 A+, 2212799 A+, 2212800 A+, 2212803 A+, 2212806 A+, 2212810 A+, 2212812 A+, 2212815 A+, 2212819 A+, 2212825 A+, 2212835 A+, 2212837 A+, 2212839 A+, 2212842 A+, 2212843 A+, 2212844 A+, 2212854 A+, 2212856 A+, 2212857 A+, 2212862 A+, 2212863 A+, 2212865 A+, 2212866 A+, 2212868 A+, 2212874 A+, 2212882 A+, 2212884 A+, 2212891 A+, 2212893 A+, 2212896 A+, 2212902 A+, 2212904 A+, 2212905 A+, 2213762 A+, 2213815 A+, 2401648 A+, 2401649 A+, 2405342 A+, 2424063 A+, 2480888 A+, 2685135 A+, 2685176 A+, 2702409 A+, 2702555 A+, 2706707 A+, 2706717 A+, 2706718 A+, 2706725 A+, 2706756 A+, 2706762 A+, 2706771 A+, 2706791 A+, 2706801 A+, 2706807 A+, 2706808 A+, 2706818 A+, 2706824 A+, 2706833 A+, 2706849 A+, 2706854 A+, 2706859 A+, 2706862 A+, 2706889 A+, 670096 A+, 680755 A+, 686634 A+, 700879 A+, 718465 A+, 720014 A+, 720232 A+, 722114 A+, 723256 A+, 723754 A+, 734319 A+, 734795 A+, 748149 A+, 903162 A+, 938336 A+, 942488 A+, 942561 A+, 942587 A+, 942610 A+, 948372 A+, 948381 A+, 948391 A+, 948393 A+, 948396 A+, 948399 A+, 948400 A+, 948404 A+, 948406 A+, 948413 A+, 948414 A+, 948418 A+, 948425 A+, 948427 A+, 948428 A+, 948445 A+, 948448 A+, 948450 A+, 948452 A+, 948458 A+, 948470 A+, 948471 A+, 948475 A+, 948478 A+, 948479 A+, 948489 A+, 948493 A+, 948494 A+, 948505 A+, 948516 A+, 948522 A+, 948524 A+, 948528 A+, 948535 A+, 948536 A+, 948540 A+, 948544 A+, 948546 A+, 948552 A+, 948554 A+, 948556 A+, 948561 A+, 948571 A+, 948580 A+, 948584 A+, 948591 A+, 948595 A+, 948601 A+, 948604 A+, 948608 A+, 948611 A+, 948617 A+, 948625 A+, 948626 A+, 948641 A+, 948653 A+, 948654 A+, 948656 A+, 948658 A+, 948667 A+, 948668 A+, 948669 A+, 948675 A+, 948678 A+, 948692 A+, 948697 A+, 948701 A+, 948709 A+, 948717 A+, 948726 A+, 948728 A+, 948740 A+, 948743 A+, 948764 A+, 948765 A+, 948768 A+, 950019 A+, 965714 A+, 966198 A+, 1244729 A++, 1596524 A++, 1631080 A++, 1631096 A++, 1631117 A++, 1631128 A++, 1631146 A++, 1631152 A++, 1631160 A++, 1631202 A++, 1631213 A++, 1631217 A++, 1631220 A++, 1631223 A++, 1631260 A++, 1631287 A++, 1631307 A++, 1631339 A++, 1631367 A++, 1631399 A++, 2212760 A++, 2212773 A++, 2212781 A++, 2212784 A++, 2212871 A++, 2424186 A++, 2702395 A++, 2702402 A++, 2702414 A++, 2702416 A++, 2702461 A++, 2706766 A++, 688731 A++, 948436 A++, 948441 A++, 948451 A++, 948459 A++, 948462 A++, 948463 A++, 948476 A++, 948487 A++, 948496 A++, 948526 A++, 948572 A++, 948593 A++, 948718 A++, 948719 A++, 1631084 A++, 1631145 A++, 1631165 A++, 1631170 A++, 1631190 A++, 1631206 A++, 1631289 A++, 1631311 A++, 1631391 A++, 2212786 A++, 2212830 A++, 2212852 A++, 2404546 A++, 2448977 A++, 2702433 A++, 2702449 A++, 2706697 A++, 2706698 A++, 2706722 A++, 2706751 A++, 942554 A++, 948433 A++, 948455 A++, 948514 A++, 948568 A++, 948602 A++, 948688 A++, 948698 A++, 950038 A++, 950042 A++, 712428 A+++, 713581 A+++, 722698 A+++, 723071 A+++, 723285 A+++, 893059 A+++, 925878 A+++, 925904 A+++, 942532 A+++, 942534 A+++, 942579 A+++, 948373 A+++, 948378 A+++, 948379 A+++, 948380 A+++, 948385 A+++, 948389 A+++, 948390 A+++, 948392 A+++, 948394 A+++, 948401 A+++, 948402 A+++, 948403 A+++, 948408 A+++, 948409 A+++, 948410 A+++, 948412 A+++, 948415 A+++, 948416 A+++, 948417 A+++, 948419 A+++, 948420 A+++, 948434 A+++, 948438 A+++, 948439 A+++, 948440 A+++, 948444 A+++, 948449 A+++, 948454 A+++, 948465 A+++, 948474 A+++, 948477 A+++, 948480 A+++, 948482 A+++, 948483 A+++, 948484 A+++, 948486 A+++, 948490 A+++, 948495 A+++, 948499 A+++, 948500 A+++, 948502 A+++, 948506 A+++, 948508 A+++, 948509 A+++, 948511 A+++, 948517 A+++, 948518 A+++, 948520 A+++, 948530 A+++, 948531 A+++, 948534 A+++, 948537 A+++, 948538 A+++, 948541 A+++, 948547 A+++, 948549 A+++, 948553 A+++, 948555 A+++, 948557 A+++, 948558 A+++, 948559 A+++, 948560 A+++, 948566 A+++, 948567 A+++, 948574 A+++, 948576 A+++, 948577 A+++, 948581 A+++, 948586 A+++, 948588 A+++, 948590 A+++, 948594 A+++, 948596 A+++, 948597 A+++, 948603 A+++, 948605 A+++, 948610 A+++, 948613 A+++, 948614 A+++, 948615 A+++, 948616 A+++, 948619 A+++, 948621 A+++, 948622 A+++, 948624 A+++, 948640 A+++, 948645 A+++, 948657 A+++, 948662 A+++, 948670 A+++, 948671 A+++, 948672 A+++, 948673 A+++, 948679 A+++, 948680 A+++, 948681 A+++, 948682 A+++, 948684 A+++, 948687 A+++, 948690 A+++, 948694 A+++, 948695 A+++, 948696 A+++, 948702 A+++, 948704 A+++, 948705 A+++, 948711 A+++, 948714 A+++, 948716 A+++, 948722 A+++, 948729 A+++, 948731 A+++, 948732 A+++, 948736 A+++, 948738 A+++, 948744 A+++, 948745 A+++, 948747 A+++, 948752 A+++, 948753 A+++, 948755 A+++, 948758 A+++, 948762 A+++, 948766 A+++, 693926 A++++, 896410 A++++, 903494 A++++, 948387 A++++, 948461 A++++, 948466 A++++, 948481 A++++, 948498 A++++, 948512 A++++, 948527 A++++, 948545 A++++, 948548 A++++, 948569 A++++, 948582 A++++, 948583 A++++, 948627 A++++, 948631 A++++, 948651 A++++, 948664 A++++, 948666 A++++, 948700 A++++, 948720 A++++, 948721 A++++, 948724 A++++
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Automatic Mining of ENzyme DAta
IFO 3070
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Manually annotated by BRENDA team
AKU 0502
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Manually annotated by BRENDA team
AKU 0502
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Manually annotated by BRENDA team
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1631157 A+, 1631173 A+, 1631216 A+, 1631234 A+, 1631235 A+, 1631304 A+, 1631312 A+, 1631326 A+, 1631348 A+, 1631362 A+, 1631432 A+, 2193765 A+, 2212794 A+, 2212810 A+, 2212813 A+, 2212848 A+, 2212854 A+, 2212869 A+, 2697499 A+, 2706760 A+, 2706788 A+, 2706811 A+, 2706813 A+, 2706834 A+, 2706836 A+, 2706850 A+, 2706863 A+, 668084 A+, 699300 A+, 713064 A+, 948321 A+, 948374 A+, 948430 A+, 948533 A+, 948551 A+, 948573 A+, 948603 A+, 948606 A+, 948635 A+, 948655 A+, 948677 A+, 948679 A+, 948767 A+, 1153605 A++, 1153609 A++, 1179289 A++, 1612215 A++, 1631037 A++, 1631043 A++, 1631044 A++, 1631045 A++, 1631053 A++, 1631058 A++, 1631063 A++, 1631066 A++, 1631070 A++, 1631082 A++, 1631087 A++, 1631141 A++, 1631237 A++, 1631387 A++, 2210884 A++, 2210898 A++, 2212702 A++, 2212703 A++, 2212704 A++, 2212713 A++, 2212716 A++, 2212719 A++, 2212723 A++, 2212728 A++, 2212732 A++, 2212737 A++, 2212740 A++, 2212741 A++, 2212764 A++, 2212777 A++, 2342921 A++, 2424989 A++, 2649333 A++, 2667499 A++, 2696828 A++, 2706655 A++, 2706662 A++, 2706663 A++, 2706675 A++, 2706676 A++, 2706677 A++, 2706684 A++, 2706685 A++, 2706690 A++, 2706699 A++, 2706708 A++, 2706711 A++, 2706724 A++, 2706754 A++, 2706757 A++, 2706884 A++, 685910 A++, 685919 A++, 685938 A++, 948320 A++, 948325 A++, 948327 A++, 948328 A++, 948334 A++, 948335 A++, 948344 A++, 948346 A++, 948350 A++, 948352 A++, 948355 A++, 948358 A++, 948359 A++, 948361 A++, 948362 A++, 948364 A++, 948501 A++, 948543 A++, 948649 A++, 948685 A++, 948750 A++, 1631048 A++, 1631096 A++, 1631278 A++, 2212715 A++, 2212729 A++, 2696784 A++, 2699726 A++, 2706679 A++, 2706688 A++, 668280 A++, 685937 A++, 948338 A++, 948341 A++, 948360 A++, 948468 A++, 675179 A+++, 948366 A+++, 948550 A+++, 948598 A+++, 948340 A++++
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Automatic Mining of ENzyme DAta
IFO 3082
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
plant
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Proteus mirabilis type S
type S
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Manually annotated by BRENDA team
Proteus vulgaris type S
type S
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Manually annotated by BRENDA team
prototype foamy virus
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Automatic Mining of ENzyme DAta
Pseudomonas aeruginosa type S
type S
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Manually annotated by BRENDA team
Pseudomonas chlororaphis type S
type S
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Manually annotated by BRENDA team
Pseudomonas cruciviae type S
type S
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Manually annotated by BRENDA team
IFO 3458
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Manually annotated by BRENDA team
ATCC 12099
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Manually annotated by BRENDA team
AKU 0820
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Manually annotated by BRENDA team
Pseudomonas putida type S
type S
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Manually annotated by BRENDA team
Pseudomonas schuylkilliensis
Pseudomonas schuylkilliensis type S
type S
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Manually annotated by BRENDA team
Pseudomonas trifolii
Pseudomonas trifolii type S
type S
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Manually annotated by BRENDA team
Ralstonia solanacearum type S
type S
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Serratia marcescens type S
type S
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Manually annotated by BRENDA team
Serratia polymuthicum
AKU 0062
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Manually annotated by BRENDA team
Serratia polymuthicum AKU 0062
AKU 0062
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
IAM 1313
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Manually annotated by BRENDA team
IAM 1671
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Manually annotated by BRENDA team
IAM 1671
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Manually annotated by BRENDA team
yeasts
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Automatic Mining of ENzyme DAta
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
human mitochondrial and nuclear DNA are edited by APOBEC3A. The degree of editing is much greater in patients lacking the uracil DNA-glycolyase gene
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-thiocytosine + H2O
2-thiouracil + NH3
show the reaction diagram
-
-
-
-
?
3-oxauracil + H2O
malonate semialdehyde + CO2 + NH3
show the reaction diagram
-
-
-
-
?
5-azacytosine + H2O
5-azauracil + NH3
show the reaction diagram
-
-
-
-
?
5-bromocytosine + H2O
5-bromouracil + NH3
show the reaction diagram
-
-
-
-
?
5-chlorocytosine + H2O
5-chlorouracil + NH3
show the reaction diagram
-
-
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
show the reaction diagram
5-iodocytosine + H2O
5-iodouracil + NH3
show the reaction diagram
-
-
-
-
?
5-methylcytosine + H2O
thymine + NH3
show the reaction diagram
6-azacytosine + H2O
6-azauracil + NH3
show the reaction diagram
creatinine + H2O
N-methylhydantoin + NH3
show the reaction diagram
cytidine + H2O
1-beta-D-ribofuranosylpyrimidin-2(1H)-one + NH3
show the reaction diagram
-
-
-
-
?
cytosine + H2O
uracil + NH3
show the reaction diagram
isocytosine + H2O
?
show the reaction diagram
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-
-
-
?
pyrimidin-2-one + H2O
(4R)-hydroxyl-3,4-dihydropyrimidine + ?
show the reaction diagram
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stabilization of a transition-state analogue at the active site of cytosine deaminase with importance of proton transfer from the Zn hydroxide group to Glu64 during the nucleophilic attack, quantum mechanical/molecular mechanical molecular dynamics and free energy simulations, active site structure, overview
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-fluorocytosine + H2O
5-fluorouracil + NH3
show the reaction diagram
5-methylcytosine + H2O
thymine + NH3
show the reaction diagram
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-
-
-
?
cytosine + H2O
uracil + NH3
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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Mn2+ reconstitutes apoenzyme after removal of Fe2+
Iron
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the enzyme contains 0.20 equivalents of Fe
KCl
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1.0 mM, relative activity 116%
NaCl
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1.0 mM, relative activity 107%
SnCl2
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1.0 mM, relative activity 122%
Zinc
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the enzyme contained 0.56 equivalents of Zn