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Literature summary for 3.5.4.1 extracted from

  • Xing, L.; Deng, X.; Kotedia, K.; Ackerstaff, E.; Ponomarev, V.; Clifton Ling, C.; Koutcher, J.A.; Li, G.C.
    Non-invasive molecular and functional imaging of cytosine deaminase and uracil phosphoribosyltransferase fused with red fluorescence protein (2008), Acta Oncol., 47, 1211-1220.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.1

Application

Application Comment Organism
pharmacology applicability of gene-directed enzyme prodrug therapy, feasibility of using magnetic resonance spectroscopy and optical imaging to measure non-invasively expression and function of cytosine deaminase in a preclinical tumor model Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression vector with cytosine deaminase fused to monomeric DsRed (mDsRed), expression vector contains immediate early promoter of cytomegalovirus (CMV) and a hygromycin B resistance gene, transient transfection of COS-7 cells Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytosine + H2O Saccharomyces cerevisiae
-
 uracil + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q12178
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 development of cell lines and animal xenograft models, expression and function of fusion constructs between cytosine deaminase and monomeric DsRed (mDsRed), analyzed by Western blot, flow cytometry, fluorescence microscopy, magnetic resonance spectroscopy, analysis of antitumoral effect of 5-fluorouracil (5-FU) and 5-fluorocytosine (5-FC) in transgenic background, increase of cytosine deaminase enhances the potential of gene-directed enzyme prodrug therapy, conversion of 5-fluorocytosine to 5-fluorouracil analyzed in mice bearing tumor xenografts with the fusion product between cytosine deaminase and monomeric DsRed (R3327-CD/mDsRed) Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-fluorocytosine + H2O recombinant enzyme in gene therapy, expression analysis in transgenic mice with xenograft tumors, optical imaging technologies Saccharomyces cerevisiae 5-fluorouracil + NH3
-
 ?
cytosine + H2O
-
 Saccharomyces cerevisiae uracil + NH3
-
 ?

Synonyms

Synonyms Comment Organism
CD
-
 Saccharomyces cerevisiae