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Literature summary for 3.5.4.1 extracted from
- Baker's yeast cytosine deaminase. Some enzymic properties and allosteric inhibition by nucleosides and nucleotides (1971), Biochemistry, 10, 4270-4276.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CDP | - |
Saccharomyces cerevisiae |  |
| CMP | - |
Saccharomyces cerevisiae | |
| CTP | - |
Saccharomyces cerevisiae | |
| cytidine | - |
Saccharomyces cerevisiae | |
| GDP | - |
Saccharomyces cerevisiae | |
| GMP | - |
Saccharomyces cerevisiae | |
| GTP | - |
Saccharomyces cerevisiae | |
| guanosine | - |
Saccharomyces cerevisiae | |
| p-mercuribenzoate | 0.3 mM, 50% inhibition | Saccharomyces cerevisiae | |
| thymidine | - |
Saccharomyces cerevisiae | |
| TTP | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
cytosine | - |
Saccharomyces cerevisiae | |
| 2.5 | - |
5-Methylcytosine | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cytosine + H2O | Escherichia coli | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Saccharomyces cerevisiae | - |
uracil + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.1 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable in the pH-range 5-9 for at least 48 h | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-methylcytosine + H2O | - |
Saccharomyces cerevisiae | thymine + NH3 | - |
? | |
| cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Saccharomyces cerevisiae | uracil + NH3 | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | stable in this range at 4°C for at least 48 h | Saccharomyces cerevisiae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
p-mercuribenzoate | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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