Any feedback?
Literature summary for 3.5.4.1 extracted from
- Computational modeling and functional analysis of Herpes simplex virus type-1 thymidine kinase and Escherichia coli cytosine deaminase fusion protein (2007), Biochem. Biophys. Res. Commun., 360, 46-50.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | the recombinant fusion enzyme HSV-1TKglyCD might be useful in cancer gene therapy | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the fusion protein HSV-1TKglyCD in HEK-293 cells and in the human lung cancer cell line NCIH460 | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a fusion protein HSV-1TKglyCD combining Herpes simplex virus type-1 thymidine kinase, HSV-1TK, and Escherichia coli cytosine deaminase, CD, with screening for the optimal suitable linker peptide, molecular modeling, overview | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ganciclovir | - |
Escherichia coli |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
recombinant fusion protein HSV-1TKglyCD | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-fluorocytosine + H2O | Escherichia coli | recombinant fusion enzyme, cytotoxicity could be enhanced by concurrently treating TKglyCD-expressing cells with prodrugs ganclocivir and 5-fluorocytosine, overview | 5-fluorouracil + NH3 | - |
? | |
| cytosine + H2O | Escherichia coli | - |
uracil + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P25524 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-fluorocytosine + H2O | - |
Escherichia coli | 5-fluorouracil + NH3 | - |
? | |
| 5-fluorocytosine + H2O | recombinant fusion enzyme, cytotoxicity could be enhanced by concurrently treating TKglyCD-expressing cells with prodrugs ganclocivir and 5-fluorocytosine, overview | Escherichia coli | 5-fluorouracil + NH3 | - |
? | |
| cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | recombinant fusion protein HSV-1TKglyCD, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
37°C, recombinant fusion protein HSV-1TKglyCD | Escherichia coli | ganciclovir | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
