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Literature summary for 3.5.4.1 extracted from
- Purification and some properties of cytosine deaminase from Salmonella typhimurium (1982), Biochim. Biophys. Acta, 791, 251-258.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium |  |
General Stability
| General Stability | Organism |
|---|---|
| exceptionally stable to denaturation | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| Cu2+ | - |
Escherichia coli | |
| Cu2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| Fe3+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| orotidine-5'-monophosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| Zn2+ | - |
Escherichia coli | |
| Zn2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.74 | - |
cytosine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
4 * 54000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| 230000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cytosine + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Escherichia coli | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Saccharomyces cerevisiae | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Salmonella enterica subsp. enterica serovar Typhimurium HD11-AE2 | - |
uracil + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
HD11-AE2 | - |
| Salmonella enterica subsp. enterica serovar Typhimurium HD11-AE2 | - |
HD11-AE2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 6.5-9, no loss of activity over a 2 week period | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-fluorocytosine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 5-fluorouracil + NH3 | - |
? | |
| 5-fluorocytosine + H2O | - |
Escherichia coli | 5-fluorouracil + NH3 | - |
? | |
| 5-fluorocytosine + H2O | - |
Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? | |
| 5-fluorocytosine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium HD11-AE2 | 5-fluorouracil + NH3 | - |
? | |
| cytosine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Saccharomyces cerevisiae | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium HD11-AE2 | uracil + NH3 | - |
? | |
| additional information | 5-methylcytosine, cytidine, deoxycytidine and CMP are no substrates | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| additional information | 5-methylcytosine, cytidine, deoxycytidine and CMP are no substrates | Salmonella enterica subsp. enterica serovar Typhimurium HD11-AE2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Escherichia coli |
| tetramer | 4 * 54000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 50 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
still active at, able to withstand high temperature denaturation | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 7.5 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
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