Information on EC 3.4.24.61 - nardilysin

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.24.61
-
RECOMMENDED NAME
GeneOntology No.
nardilysin
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolysis of polypeptides, preferably at -Xaa-/-Arg-Lys-, and less commonly at -Arg-/-Arg-Xaa-, in which Xaa is not Arg or Lys
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
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-
CAS REGISTRY NUMBER
COMMENTARY hide
157906-54-2
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292850-69-2
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminobenzoyl-GFSPFRSSRQ-ED(2,4-dinitrophenyl) + H2O
?
show the reaction diagram
-
-
-
?
2-aminobenzoyl-GGALRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGA + LRKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 24% at AL and 76% at RK
-
-
?
2-aminobenzoyl-GGALRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGALR + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 24% at AL and 76% at RK
-
-
?
2-aminobenzoyl-GGFARKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFA + RKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 65% at AR and 35% at RK
-
-
?
2-aminobenzoyl-GGFARKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFAR + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 65% at AR and 35% at RK
-
-
?
2-aminobenzoyl-GGFFRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFF + RKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 100% at FR
-
-
?
2-aminobenzoyl-GGFHRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFH + RKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 100% at HR
-
-
?
2-aminobenzoyl-GGFIKKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFIK + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 0% at IK and 100% at KK
-
-
?
2-aminobenzoyl-GGFIKRVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFIK + RVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 0% at IK and 100% at KR
-
-
?
2-aminobenzoyl-GGFIRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFIR + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
2-aminobenzoyl-GGFIRRVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFIR + RVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 0% at IR and 100% at RR
-
-
?
2-aminobenzoyl-GGFLKKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFL + KKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 5% at LL and 95% at KK
-
-
?
2-aminobenzoyl-GGFLKKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLK + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 5% at LL and 95% at KK
-
-
?
2-aminobenzoyl-GGFLKRVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFL + KRVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 1% at LK and 99% at KR
-
-
?
2-aminobenzoyl-GGFLKRVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLK + RVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 1% at LK and 99% at KR
-
-
?
2-aminobenzoyl-GGFLRKAGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLR + KAGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 25% at KA and 75% at RK
-
-
?
2-aminobenzoyl-GGFLRKAGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLRK + AGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 25% at KA and 75% at RK
-
-
?
2-aminobenzoyl-GGFLRKDGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLR + KDGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 23% at KD and 77% at RK
-
-
?
2-aminobenzoyl-GGFLRKDGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLRK + DGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 23% at KD and 77% at RK
-
-
?
2-aminobenzoyl-GGFLRKFGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLR + KFGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 17% at KF and 83% at RK
-
-
?
2-aminobenzoyl-GGFLRKFGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLRK + FGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 17% at KF and 83% at RK
-
-
?
2-aminobenzoyl-GGFLRKHGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLR + KHGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 10% at KH and 90% at RK
-
-
?
2-aminobenzoyl-GGFLRKHGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLRK + HGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 10% at KH and 90% at RK
-
-
?
2-aminobenzoyl-GGFLRKIGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLR + KIGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 19% at KI and 81% at RK
-
-
?
2-aminobenzoyl-GGFLRKIGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLRK + IGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 19% at KI and 81% at RK
-
-
?
2-aminobenzoyl-GGFLRKLGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLR + KLGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 26% at KL and 74% at RK
-
-
?
2-aminobenzoyl-GGFLRKLGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLRK + LGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 26% at KL and 74% at RK
-
-
?
2-aminobenzoyl-GGFLRKMGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLR + KMGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 14% at KM and 86% at RK
-
-
?
2-aminobenzoyl-GGFLRKMGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLRK + MGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 14% at KM and 86% at RK
-
-
?
2-aminobenzoyl-GGFLRKNGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLR + KNGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 24% at KN and 76% at RK
-
-
?
2-aminobenzoyl-GGFLRKNGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLRK + NGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 24% at KN and 76% at RK
-
-
?
2-aminobenzoyl-GGFLRKSGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLR + KSGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 25% at KS and 75% at RK
-
-
?
2-aminobenzoyl-GGFLRKSGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLRK + SGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 25% at KS and 75% at RK
-
-
?
2-aminobenzoyl-GGFLRKVGQ-(N-2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-GGFL + RKVGQ-(N-2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
percentage cleaved 23% at LR and 77% at RK
-
-
?
2-aminobenzoyl-GGFLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGF + LRKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 23% at FL and 77% at RK
-
-
?
2-aminobenzoyl-GGFLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFL + RKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 23% at LR and 77% at RK
-
-
?
2-aminobenzoyl-GGFLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLR + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
2-aminobenzoyl-GGFLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLRK + VGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 23% at KV and 77% at RK
-
-
?
2-aminobenzoyl-GGFLRKWGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLR + KWGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 13% at KW and 87% at RK
-
-
?
2-aminobenzoyl-GGFLRKWGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLRK + WGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 13% at KW and 87% at RK
-
-
?
2-aminobenzoyl-GGFLRRVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFL + RRVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 7% at LR and 93% at RR
-
-
?
2-aminobenzoyl-GGFLRRVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFLR + RVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 7% at LR and 93% at RR
-
-
?
2-aminobenzoyl-GGFLRRVGQ-N[2,4-dinitrophenyl]-ethylenediamine + H2O
?
show the reaction diagram
-
-
-
?
2-aminobenzoyl-GGFMRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFM + RKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 29% at MR and 71% at RK
-
-
?
2-aminobenzoyl-GGFMRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFMR + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 29% at MR and 71% at RK
-
-
?
2-aminobenzoyl-GGFNRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFN + RKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 100% at NR
-
-
?
2-aminobenzoyl-GGFSRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFS + RKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 76% at SR and 24% at RK
-
-
?
2-aminobenzoyl-GGFSRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFSR + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 76% at SR and 24% at RK
-
-
?
2-aminobenzoyl-GGFVRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFVR + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 100% at RK
-
-
?
2-aminobenzoyl-GGFWRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFW + RKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 100% at WR
-
-
?
2-aminobenzoyl-GGFYKKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFYKKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 100% at YK and 0% at KK
-
-
?
2-aminobenzoyl-GGFYKRVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFY + KRVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 64% at YK and 36% at KR
-
-
?
2-aminobenzoyl-GGFYKRVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFYK + RVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 64% at YK and 36% at KR
-
-
?
2-aminobenzoyl-GGFYRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFY + RKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
2-aminobenzoyl-GGFYRRVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFY + RRVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 65% at YR and 35% at RR
-
-
?
2-aminobenzoyl-GGFYRRVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGFYR + RVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 65% at YR and 35% at RR
-
-
?
2-aminobenzoyl-GGHLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGH + LRKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 26% at HL and 74% at RK
-
-
?
2-aminobenzoyl-GGHLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGHLR + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 26% at HL and 74% at RK
-
-
?
2-aminobenzoyl-GGILRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGI + LRKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 56% at IL and 44% at RK
-
-
?
2-aminobenzoyl-GGILRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGILR + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 56% at IL and 44% at RK
-
-
?
2-aminobenzoyl-GGLLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGL + LRKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 73% at LL and 27% at RK
-
-
?
2-aminobenzoyl-GGLLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGLLR + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 73% at LL and 27% at RK
-
-
?
2-aminobenzoyl-GGMLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGM + LRKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 81% at ML and 19% at RK
-
-
?
2-aminobenzoyl-GGMLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGMLR + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 81% at ML and 19% at RK
-
-
?
2-aminobenzoyl-GGNLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGN + LRKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 23% at NL and 77% at RK
-
-
?
2-aminobenzoyl-GGNLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGNLR + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 23% at NL and 77% at RK
-
-
?
2-aminobenzoyl-GGSLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGS + LRKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 4% at SL and 96% at RK
-
-
?
2-aminobenzoyl-GGSLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGSLR + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 4% at SL and 96% at RK
-
-
?
2-aminobenzoyl-GGVLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGV + LRKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 19% at VL and 81% at RK
-
-
?
2-aminobenzoyl-GGVLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGVLR + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 19% at VL and 81% at RK
-
-
?
2-aminobenzoyl-GGWLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGW + LRKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 18% at WL and 82% at RK
-
-
?
2-aminobenzoyl-GGWLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGWLR + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 18% at WL and 82% at RK
-
-
?
2-aminobenzoyl-GGYLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGY + LRKVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 2% at YL and 98% at RK
-
-
?
2-aminobenzoyl-GGYLRKVGQ-ethylenediamine-2,4-dinitrophenyl + H2O
2-aminobenzoyl-GGYLR + KVGQ-ethylenediamine-2,4-dinitrophenyl
show the reaction diagram
-
percentage cleaved 2% at YL and 98% at RK
-
-
?
2-aminobenzoyl-MISLMKRPPGFSPFRSSRI-NH2 + H2O
desArg10 kallidin (KRPPGFSPF) + ?
show the reaction diagram
-
kallidin model peptide, nardilysin does not release desArg10 kallidin from the physiological precursor low molecular weight kiniogen
-
?
2-aminobenzoyl-MISLMKRPQ-ED(2,4-dinitrophenyl) + H2O
?
show the reaction diagram
-
-
-
?
2-aminobenzoyl-TPLVTLX1X2NAIIKQED(2,4-dinitrophenyl) + H2O
?
show the reaction diagram
-
fluorogenic substrate based on beta-endorphin, nardilysin hydrolyzes peptides when X1-X2 are F-K, F-R, W-K, M-K,Y-K, or L-K, K-F is not hydrolyzed
-
?
Abz-GGFX(1)X(2)X(3)VGQ-EDDnp + H2O
?
show the reaction diagram
-
X residues are varied
-
?
Abz-GGGFLRRVGQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
alpha-natriuretic factor ANF + H2O
?
show the reaction diagram
-
-
-
?
alpha-neoendorphin + H2O
?
show the reaction diagram
-
-
-
-
?
alpha-Neoendorphin + H2O
Tyr-Gly-Gly-Phe-Leu-Arg + Lys-Tyr-Pro-Leu
show the reaction diagram
amyloid precursor protein + H2O
?
show the reaction diagram
-
the enzyme enhances alpha-cleavage of amyloid precursor protein, which results in the decreased generation of amyloid beta in vitro
-
-
?
ANF-(1-28) + H2O
?
show the reaction diagram
-
i.e. atrial natriuretic factor, cleavage site: Arg+Arg
-
-
-
beta-endorphin + H2O
Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-Phe + Lys-Asn-Ala-Ile-Ile-Lys-Asn-Ala-Tyr-Lys-Lys-Gly-Gln + ?
show the reaction diagram
-
-
-
?
Bovine adrenal medulla dodecapeptide + H2O
Tyr-Gly-Gly-Phe-Met-Arg + Arg-Val-Gly-Arg-Pro-Glu
show the reaction diagram
calcitonin + H2O
?
show the reaction diagram
-
-
-
?
dynorphin A + H2O
?
show the reaction diagram
dynorphin A + H2O
Tyr-Gly-Gly-Phe-Leu-Arg + Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln-NH2
show the reaction diagram
dynorphin A-(1-10) + H2O
Tyr-Gly-Gly-Phe-Leu-Arg + Arg-Ile-Arg-Pro
show the reaction diagram
dynorphin A-(1-13) + H2O
Tyr-Gly-Gly-Phe-Leu-Arg + Arg-Ile-Arg-Pro-Lys-Leu-Lys
show the reaction diagram
dynorphin A-(1-9) + H2O
Tyr-Gly-Gly-Phe-Leu-Arg + Arg-Ile-Arg
show the reaction diagram
dynorphin B + H2O
Tyr-Gly-Gly-Phe-Leu-Arg + Arg-Gln-Phe-Lys-Val-Val-Thr
show the reaction diagram
Glucagon + H2O
?
show the reaction diagram
-
-
-
-
?
glucagon + H2O
miniglucagon + ?
show the reaction diagram
heparin-binding epidermal growth factor-like growth factor + H2O
?
show the reaction diagram
substrate is synthesized as a transmembrane protein. Enzyme enhances ectodomain shedding of substrate in coperation with tumor necrosis factor-alpha-converting enzyme. Metalloendopeptidase activity of enzyme is not required for enhancement of shedding
-
-
?
malate dehydrogenase + H2O
?
show the reaction diagram
-
-
-
-
?
N-(2-(N-methylamino)benzoyl)-GGFLRRVGK(2,4-dinitrophenyl)-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
peptide I + H2O
Pro-Arg-Glu-Arg-Lys + Ala-Gly-Ala-Lys-Asn-Tyr-NH2
show the reaction diagram
-
i.e. Pro-Arg-Glu-Arg-Lys-Ala-Gly-Ala-Lys-Asn125I-Tyr-NH2
i.e. peptide II
-
Peptide III + H2O
Pro-Arg-Glu-Arg + Arg-Ala-Gly-Ala-Lys-Asn-Tyr-NH2
show the reaction diagram
-
i.e. Pro-Arg-Glu-Arg-Arg-Ala-Gly-Ala-Lys-Asn-Tyr-NH2
-
-
PGC-1alpha + H2O
?
show the reaction diagram
nardilysin and PGC-1alpha interact and colocalize at the uncoupling protein 1 enhancer, where the enzyme represses PGC-1alpha activity
-
-
?
PRAME190-198 + H2O
?
show the reaction diagram
-
nardilysin produces precursors of tumor-associated protein PRAME(190-198) by producing cleavage after Lys200, Arg201 and Lys202 but does not excise precisely at the C terminus (Lys198)
-
-
?
Preproneurotensin-(154-170) + H2O
?
show the reaction diagram
Pro-Gln-Arg-Lys-Tyr-NH2 + H2O
Pro-Gln + Arg-Lys-Tyr-NH2
show the reaction diagram
-
-
-
-
Pro-Glu-Arg-Arg-Tyr-NH2 + H2O
Pro-Glu + Arg-Arg-Tyr-NH2
show the reaction diagram
-
-
-
-
Pro-Gly-Arg-Lys-Tyr-NH2 + H2O
Pro-Gly + Arg-Lys-Tyr-NH2
show the reaction diagram
-
-
-
-
pro-tumour necrosis factor-alpha + H2O
?
show the reaction diagram
-
-
-
-
?
somatostatin-28 + H2O
?
show the reaction diagram
Somatostatin-28 + H2O
Somatostatin-28-(13-28) + somatostatin-28-(1-12)
show the reaction diagram
tumor necrosis factor-alpha + H2O
?
show the reaction diagram
-
-
-
-
?
[Ala17,Tyr20]-Somatostatin-28-(10-20)-NH2 + H2O
Pro-Arg-Glu + Arg-Lys-Ala-Gly-Ala-Lys-Asn-Tyr-NH2
show the reaction diagram
[Arg0,Ala17]-somatostatin-28-(1-19)-NH2 + H2O
?
show the reaction diagram
-
-
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-neoendorphin + H2O
?
show the reaction diagram
-
-
-
-
?
amyloid precursor protein + H2O
?
show the reaction diagram
-
the enzyme enhances alpha-cleavage of amyloid precursor protein, which results in the decreased generation of amyloid beta in vitro
-
-
?
dynorphin A + H2O
?
show the reaction diagram
-
-
-
-
?
Glucagon + H2O
?
show the reaction diagram
-
-
-
-
?
heparin-binding epidermal growth factor-like growth factor + H2O
?
show the reaction diagram
O43847
substrate is synthesized as a transmembrane protein. Enzyme enhances ectodomain shedding of substrate in coperation with tumor necrosis factor-alpha-converting enzyme. Metalloendopeptidase activity of enzyme is not required for enhancement of shedding
-
-
?
malate dehydrogenase + H2O
?
show the reaction diagram
-
-
-
-
?
PGC-1alpha + H2O
?
show the reaction diagram
Q8BHG1
nardilysin and PGC-1alpha interact and colocalize at the uncoupling protein 1 enhancer, where the enzyme represses PGC-1alpha activity
-
-
?
pro-tumour necrosis factor-alpha + H2O
?
show the reaction diagram
-
-
-
-
?
somatostatin-28 + H2O
?
show the reaction diagram
-
-
-
-
?
tumor necrosis factor-alpha + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
2-aminobenzoyl-GGFIRRVGQ-EDDnp
-
alternate substrate inhibitor
2-aminobenzoyl-GGFLRRVGQ-N[2,4-dinitrophenyl]-ethylenediamine
-
substrate inhibitionl
amastatin
bestatin
beta-endorphin
-
substrate inhibitor
Chelating agents
-
strong
N-ethylmaleimide
N-tosyl-L-Phe chloromethyl ketone
NaCl
-
-
Phosphate buffer
-
-
-
spermine
-
-
TAPI-2
-
hydroxamic-based inhibitor
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
p42IP4
-
p42IP4 (centaurin alpha1) enhances the enzymatic activity of NRD 3-4 times.
-
additional information
-
increase in enzyme activity by 50% upon formation of complex with mitochondrial malate dehydrogenase
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0019
2-aminobenzoyl-GFSPFRSSRQED(2,4-dinitrophenyl)
-
pH 7.0, 37C
0.0074
2-aminobenzoyl-GGALRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0061
2-aminobenzoyl-GGFARKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0094
2-aminobenzoyl-GGFFRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0044
2-aminobenzoyl-GGFHRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0075
2-aminobenzoyl-GGFIKKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFIXXVGQ-ethylenediamine-2,4-dinitrophenyl
0.0074
2-aminobenzoyl-GGFIKRVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFIXXVGQ-ethylenediamine-2,4-dinitrophenyl
0.0062 - 0.0072
2-aminobenzoyl-GGFIRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0033
2-aminobenzoyl-GGFIRRVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFIXXVGQ-ethylenediamine-2,4-dinitrophenyl
0.0014
2-aminobenzoyl-GGFIRRVGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 7.0, 37C
0.0073
2-aminobenzoyl-GGFLKKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLXXVGQ-ethylenediamine-2,4-dinitrophenyl
0.0051
2-aminobenzoyl-GGFLKRVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLXXVGQ-ethylenediamine-2,4-dinitrophenyl
0.01
2-aminobenzoyl-GGFLRKAGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
0.011
2-aminobenzoyl-GGFLRKDGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
0.0066
2-aminobenzoyl-GGFLRKFGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
0.0048
2-aminobenzoyl-GGFLRKHGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
0.0051
2-aminobenzoyl-GGFLRKIGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
0.0049
2-aminobenzoyl-GGFLRKLGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
0.0075
2-aminobenzoyl-GGFLRKMGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
0.0097
2-aminobenzoyl-GGFLRKNGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
0.0101
2-aminobenzoyl-GGFLRKSGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
0.0057 - 0.0065
2-aminobenzoyl-GGFLRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0055
2-aminobenzoyl-GGFLRKWGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
0.0036
2-aminobenzoyl-GGFLRRVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLXXVGQ-ethylenediamine-2,4-dinitrophenyl
0.00023 - 0.03
2-aminobenzoyl-GGFLRRVGQ-N[2,4-dinitrophenyl]-ethylenediamine
0.0072
2-aminobenzoyl-GGFMRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.003
2-aminobenzoyl-GGFNRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.004
2-aminobenzoyl-GGFSRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0095
2-aminobenzoyl-GGFVRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0053
2-aminobenzoyl-GGFWRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0039
2-aminobenzoyl-GGFYKKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFYXXVGQ-ethylenediamine-2,4-dinitrophenyl
0.003
2-aminobenzoyl-GGFYKRVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFYXXVGQ-ethylenediamine-2,4-dinitrophenyl
0.0083 - 0.0094
2-aminobenzoyl-GGFYRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0026
2-aminobenzoyl-GGFYRRVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFYXXVGQ-ethylenediamine-2,4-dinitrophenyl
0.0057
2-aminobenzoyl-GGHLRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0064
2-aminobenzoyl-GGILRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0091
2-aminobenzoyl-GGLLRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0067
2-aminobenzoyl-GGMLRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0054
2-aminobenzoyl-GGNLRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0065
2-aminobenzoyl-GGSLRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0092
2-aminobenzoyl-GGVLRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.008
2-aminobenzoyl-GGWLRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0092
2-aminobenzoyl-GGYLRKVGQ-ethylenediamine-2,4-dinitrophenyl
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.0041
2-aminobenzoyl-MISLMKRPPGFSPFRSSRINH2
-
pH 7.0, 37C
0.0035
2-aminobenzoyl-MISLMKRPQED(2,4-dinitrophenyl)
-
pH 7.0, 37C
0.00054 - 0.0466
2-aminobenzoyl-TPLVTLX1X2NAIIKQED(2,4-dinitrophenyl)
0.974
alpha-neoendorphin
-
-
0.00625
ANF-(1-28)
-
-
0.0014
betaendorphin
-
pH 7.0, 37C
0.103
Bovine adrenal medulla dodecapeptide
-
-
0.0017
calcitonin
-
pH 7.0, 37C
0.00645
dynorphin A
-
-
0.127
dynorphin A-(1-10)
-
-
0.024
dynorphin A-(1-13)
-
-
0.162
dynorphin A-(1-9)
-
-
0.209
Dynorphin B
-
-
0.002
dynorphin B13
-
pH 7.0, 37C
-
0.0173
Preproneurotensin-(154-170)
-
-
0.0005 - 0.008
Somatostatin-14
0.0055 - 0.043
Somatostatin-28
0.428
[Ala17,Tyr20]-Somatostatin-28-(10-20)-NH2
0.227
[Arg0,Ala17]-somatostatin-28-(1-19)-NH2
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.031 - 7.68
2-aminobenzoyl-GFSPFRSSRQED(2,4-dinitrophenyl)
3.88
2-aminobenzoyl-GGALRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
1.08
2-aminobenzoyl-GGFARKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl
2.83
2-aminobenzoyl-GGFFRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl
6.9
2-aminobenzoyl-GGFHRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.198
2-aminobenzoyl-GGFIKKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFIXXVGQ-ethylenediamine-2,4-dinitrophenyl
12.09
2-aminobenzoyl-GGFIKRVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFIXXVGQ-ethylenediamine-2,4-dinitrophenyl
1.22 - 1.23
2-aminobenzoyl-GGFIRKVGQ-ethylenediamine-2,4-dinitrophenyl
15.73
2-aminobenzoyl-GGFIRRVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFIXXVGQ-ethylenediamine-2,4-dinitrophenyl
3.3 - 5.03
2-aminobenzoyl-GGFIRRVGQ-N-(2,4-dinitrophenyl)ethylenediamine
2.16
2-aminobenzoyl-GGFLKKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLXXVGQ-ethylenediamine-2,4-dinitrophenyl
9.75
2-aminobenzoyl-GGFLKRVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLXXVGQ-ethylenediamine-2,4-dinitrophenyl
2.04
2-aminobenzoyl-GGFLRKAGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
0.24
2-aminobenzoyl-GGFLRKDGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
2.94
2-aminobenzoyl-GGFLRKFGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
13.69
2-aminobenzoyl-GGFLRKHGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
2.04
2-aminobenzoyl-GGFLRKIGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
1.74
2-aminobenzoyl-GGFLRKLGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
2.77
2-aminobenzoyl-GGFLRKMGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
1.59
2-aminobenzoyl-GGFLRKNGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
4.65
2-aminobenzoyl-GGFLRKSGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
2.48
2-aminobenzoyl-GGFLRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl and hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl and hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl and hydrolysis of the peptide series 2-aminobenzoyl-GGFLXXVGQ-ethylenediamine-2,4-dinitrophenyl
5.61
2-aminobenzoyl-GGFLRKWGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLRKXGQ-ethylenediamine-2,4-dinitrophenyl
9.92
2-aminobenzoyl-GGFLRRVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFLXXVGQ-ethylenediamine-2,4-dinitrophenyl
4.86
2-aminobenzoyl-GGFMRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl
1.08
2-aminobenzoyl-GGFNRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.353
2-aminobenzoyl-GGFSRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.41
2-aminobenzoyl-GGFVRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.705
2-aminobenzoyl-GGFWRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFXRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.187
2-aminobenzoyl-GGFYKKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFYXXVGQ-ethylenediamine-2,4-dinitrophenyl
0.405
2-aminobenzoyl-GGFYKRVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFYXXVGQ-ethylenediamine-2,4-dinitrophenyl
2.59 - 2.63
2-aminobenzoyl-GGFYRKVGQ-ethylenediamine-2,4-dinitrophenyl
18.99
2-aminobenzoyl-GGFYRRVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGFYXXVGQ-ethylenediamine-2,4-dinitrophenyl
4.36
2-aminobenzoyl-GGHLRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
4.84
2-aminobenzoyl-GGILRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
5.83
2-aminobenzoyl-GGLLRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
7.84
2-aminobenzoyl-GGMLRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.953
2-aminobenzoyl-GGNLRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
8.53
2-aminobenzoyl-GGSLRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
3.24
2-aminobenzoyl-GGVLRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
4.95
2-aminobenzoyl-GGWLRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
1.85
2-aminobenzoyl-GGYLRKVGQ-ethylenediamine-2,4-dinitrophenyl
Rattus norvegicus
-
pH 7.0, hydrolysis of the peptide series 2-aminobenzoyl-GGXLRKVGQ-ethylenediamine-2,4-dinitrophenyl
0.04 - 9.48
2-aminobenzoyl-MISLMKRPQED(2,4-dinitrophenyl)
0.216 - 6.08
2-aminobenzoyl-TPLVTLX1X2NAIIKQED(2,4-dinitrophenyl)
3.3 - 5.03
beta-endorphin
1.81 - 2.94
calcitonin
0.783 - 6.08
somatostatin14
additional information
2-aminobenzoyl-MISLMKRPPGFSPFRSSRI-NH2
Mus musculus
-
pH 7.0, 37C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
second minor pH-optimum
7
-
assay at
8.5
-
peptide I as substrate
8.9
-
[Ala17,Tyr20]-somatostatin-28-(10-20)-NH2 as substrate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9.8
-
enzyme is irreversibly inactivated below pH 6.0 and above pH 9.8
7.3 - 9
-
about half-maximal activity at pH 7.3 and about 90% of maximal activity at pH 9, peptide I as substrate
8.2 - 9.3
-
[Ala17,Tyr20]-somatostatin-28-(10-20)-NH2 as substrate, about half-maximal activity at pH 8.2 and 9.3
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
neuronal cells
Manually annotated by BRENDA team
-
nuclei, neuronal cells
Manually annotated by BRENDA team
-
Purkinje neurons and dendrites
Manually annotated by BRENDA team
-
mRNA isolated from
Manually annotated by BRENDA team
-
in cortical neurons the enzyme is partially co-localized with parvalbumin, but not with calretinin
Manually annotated by BRENDA team
-
NRD is expressed in the neurites of RA-stimulated SHSY5Y cells, and localized in vesicular structures
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
p42IP4 (centaurin-alpha), nardilysin and tubulin are found in the cytosol and at the plasma membrane of SH-SY5Y neuroblastoma cells
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
105000
-
protease V8 digestion generates a stable form with the same properties as the uncleaved enzyme
110000
128000
-
-
130000
-
-
133000
140000
187000
-
purified enzyme, gel filtration
300000
-
heterooligomer containing enzyme, testis or brain extract, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 110000, testis, SDS-PAGE, 1 * 140000, brain, SDS-PAGE
additional information
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 9
-
3-15 h, 25% or less loss of activity
31367
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52
-
half-life of 5.5 min at pH 6.5
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 catalytically active forms
-
recombinant enzyme expressed in Sf9 cells
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of the wild-type enzyme and the mutant E235A in COS-7 or HEK-293T cells, co-expression with ADAM17
-
full-length cDNA from a testis library
-
heterologous expression of hNRDc1 by both human myometrial smooth muscle cells and ltk- mouse fibroblasts. Analysation of the regulatory function of hNRDc1 regarding mK44 (a splice variant of maxi-K chanel). Data indicate that hNRDc1 is involved in regulating the excitation of smooth muscle cells.
-
isolation of cDNA
-
overexpression of V5-tagged NRDc from a a lentivirus vector in TACE-deficient fibroblasts, co-expression of NRDc with ADAM10, recombinant wild-type NRDc and mutant E235A are synthesized using a Bac-to-Bac baculovirus expression system, co-expression of the C-terminally V5-tagged wild-type enzyme with the TNF-alpha-converting enzyme in COS-7 cells
-
rat, testis
-
recombinant enzyme expressed in Sf9 cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
differentiation of SH-SY5Y cells by stimulation with 10 microM retinoic acid (RA) results in upregulation of NRD protein levels, with a 6fold rise after 15 days
-
protein expression is frequently elevated both in the serum and cancer epithelium of gastric cancer patients
-
the enzyme is highly expressed in neuroblastoma cells
-
there is strongly decreased neuronal enzyme expression in Down syndrome. The enzyme is down-regulated (about 1.21fold) in brains of individuals with bipolar disorder
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C948A
-
the mutation of a highly conserved cysteine residue in NRD to Ala abolishes the potentiation by tubulin
DELTA1-148
-
mutant does not bind the H3K4me2 peptide
DELTA1-260
-
mutant binds the H3K4me2 peptide
DELTA1-445
-
mutant binds the H3K4me2 peptide
DELTA1-621
-
mutant binds the H3K4me2 peptide
DELTA149-621
-
mutant does not bind the H3K4me2 peptide
E235A
-
inactive mutant
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
the enzyme plays a role in the pathophysiology of schizophrenia