Information on EC 3.1.30.1 - Aspergillus nuclease S1

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY
3.1.30.1
-
RECOMMENDED NAME
GeneOntology No.
Aspergillus nuclease S1
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products
show the reaction diagram
-
-
-
-
Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products
show the reaction diagram
endonuclease I catalyzes the breakage of the P-O3' bond
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of phosphoric diester
-
-
hydrolysis of phosphoric ester
-
-
-
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Alteromonas BAL 31 nuclease
-
-
Aspergillus oryzae S1 nuclease
-
-
-
-
Bacillus subtilis deoxyribonuclease
-
-
Bacillus subtilis deoxyribonuclease
Bacillus subtilis Marburg
-
-
-
CEL I
Q9LL59
-
Deoxyribonuclease P1
-
-
-
-
deoxyribonuclease S1
-
-
-
-
EC 3.1.4.21
-
formerly
Endonuclease P1
-
-
-
-
Endonuclease S1
-
-
-
-
endonuclease S1 (Aspergillus)
-
-
-
-
KB endonuclease
-
-
M1 nuclease
-
-
mung bean endonuclease
-
-
mung bean nuclease
-
-
Neurospora crassa endonuclease
-
-
Neurospora crassa nuclease
-
-
Neurospora crassa single-strand specific endonuclease
-
-
Phage T4 endonuclease IV
-
-
Physarum polycephalum nuclease
-
-
Pisum sativum endonuclease
-
-
resolvase T7
-
-
rye germ nuclease
-
-
S1-nuclease
-
-
S1-type nuclease
-
-
Schizosaccharomyces pombe endonuclease
-
-
single strand preferring nuclease
-
-
single-strand endodeoxyribonuclease
-
-
-
-
single-strand-preferring nuclease (SSP nuclease)
-
-
single-strand-specific DNase
-
-
single-strand-specific endodeoxyribonuclease
-
-
-
-
single-strand-specific nuclease
-
-
single-strand-specific nuclease
-
-
single-strand-specific S1 endonuclease
-
-
single-stranded DNA specific endonuclease
-
-
-
-
single-stranded-nucleate endonuclease
-
-
-
-
SSPN
-
-
T7 endonuclease
-
-
Ustilago maydis nuclease
-
-
wheat seedling nuclease
-
-
CAS REGISTRY NUMBER
COMMENTARY
37288-25-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
celery
SwissProt
Manually annotated by BRENDA team
Marburg strain
-
-
Manually annotated by BRENDA team
Bacillus subtilis Marburg
Marburg strain
-
-
Manually annotated by BRENDA team
plasmid RK2 ParB protein
-
-
Manually annotated by BRENDA team
isolated from colonial ascidium collected near Shikotan Island, Sea of Okhotsk, at a depth of 123 m
-
-
Manually annotated by BRENDA team
cv. Chinese Spring
-
-
Manually annotated by BRENDA team
mung bean
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
-
tight correlation found between the extracellular endonuclease activity and the rate of thymidine uptake by actively growing cells suggesting that this nuclease is required for fulfilling the nucleotide pool of precursors of DNA biosynthesis during the transformation of hyphae into the aerial mycelium and conidia in stressful environmental conditions
additional information
-
mode of nuclease action and molecular modeling, overview
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
?
2'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
3'-CMP + H2O
cytosine + phosphate
show the reaction diagram
-
-
-
?
3'-CMP + H2O
cytosine + phosphate
show the reaction diagram
-
-
-
?
3'-CMP + H2O
cytosine + phosphate
show the reaction diagram
-
-
-
?
3'-CMP + H2O
cytosine + phosphate
show the reaction diagram
-
-
-
?
3'-CMP + H2O
cytosine + phosphate
show the reaction diagram
-
-
-
?
3'-CMP + H2O
cytosine + phosphate
show the reaction diagram
-
-
-
?
3'-dAMP + H2O
deoxyadenosine + phosphate
show the reaction diagram
-
-
-
?
3'-dAMP + H2O
deoxyadenosine + phosphate
show the reaction diagram
-
-
-
?
3'-dAMP + H2O
deoxyadenosine + phosphate
show the reaction diagram
-
-
-
?
3'-dAMP + H2O
deoxyadenosine + phosphate
show the reaction diagram
-
-
-
?
3'-dAMP + H2O
deoxyadenosine + phosphate
show the reaction diagram
-
only little activity
-
?
3'-dCMP + H2O
deoxycytosine + phosphate
show the reaction diagram
-
-
-
?
3'-dCMP + H2O
deoxycytosine + phosphate
show the reaction diagram
-
-
-
?
3'-dCMP + H2O
deoxycytosine + phosphate
show the reaction diagram
-
-
-
?
3'-dGMP + H2O
deoxyguanosine + phosphate
show the reaction diagram
-
-
-
?
3'-dGMP + H2O
deoxyguanosine + phosphate
show the reaction diagram
-
-
-
?
3'-dGMP + H2O
deoxyguanosine + phosphate
show the reaction diagram
-
-
-
?
3'-dTMP + H2O
deoxythymidine + phosphate
show the reaction diagram
-
-
-
?
3'-dTMP + H2O
deoxythymidine + phosphate
show the reaction diagram
-
-
-
?
3'-dTMP + H2O
deoxythymidine + phosphate
show the reaction diagram
-
-
-
?
3'-dTMP + H2O
deoxythymidine + phosphate
show the reaction diagram
-
-
-
?
3'-GMP + H2O
guanosine + phosphate
show the reaction diagram
-
-
-
?
3'-GMP + H2O
guanosine + phosphate
show the reaction diagram
-
-
-
?
3'-GMP + H2O
guanosine + phosphate
show the reaction diagram
-
-
-
?
3'-GMP + H2O
guanosine + phosphate
show the reaction diagram
-
-
-
?
3'-GMP + H2O
guanosine + phosphate
show the reaction diagram
-
-
-
?
3'-GMP + H2O
guanosine + phosphate
show the reaction diagram
-
-
-
?
3'-UMP + H2O
uridine + phosphate
show the reaction diagram
-
-
-
?
3'-UMP + H2O
uridine + phosphate
show the reaction diagram
-
-
-
?
3'-UMP + H2O
uridine + phosphate
show the reaction diagram
-
-
-
?
3'-UMP + H2O
uridine + phosphate
show the reaction diagram
-
-
-
?
3'-UMP + H2O
uridine + phosphate
show the reaction diagram
-
-
-
?
adenosine 3'-diphosphate 5'-phosphate
5'-AMP + diphosphate
show the reaction diagram
-
-
-
?
adenosine 3'5'-biphosphate + H2O
5'-AMP + phosphate
show the reaction diagram
-
-
-
?
adenosine 3'5'-biphosphate + H2O
5'-AMP + phosphate
show the reaction diagram
-
-
-
?
ApA + H2O
adenosine + AMP
show the reaction diagram
-
-
-
?
bis(p-nitrophenyl)phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
almost no hydrolysis
-
?
deoxythymidine 3',5'-bisphosphate
5'-dTMP + phosphate
show the reaction diagram
-
-
-
?
DNA + H2O
?
show the reaction diagram
-
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
functional role in recombination and DNA repair in vivo
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
75000 times more active on single-stranded than on native DNA
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
native DNA hydrolysis probably by contaminating nuclease, its inactivation possible at 55C
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
at acidic pH the enzyme is 25times more active on single-stranded DNA than on double-stranded DNA and 3times more active on single-stranded DNA than on single-stranded RNA. At neutral pH the RNase activity exceeds the DNase activity. The enzyme nicks supercoiled RF-I plasmid DNA and rapidly cuts the phosphodiester bond across from the nick in the resultant relaxed RF-II plasmid DNA.
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
Bacillus subtilis Marburg
-
-
-
?
DNA + H2O
nicked DNA
show the reaction diagram
-
-
-
-
?
DNA + H2O
nicked DNA
show the reaction diagram
-
resolving of DNA-four way junction
-
-
?
DNA + H2O
nicked DNA
show the reaction diagram
-
resolving of four-way junction
-
-
?
DNA + H2O
nicked DNA
show the reaction diagram
-
T7 endonuclase I resolves four-way junctions by simultaeously introducing two nicks on the two continously stacked strands at sites 5' to the junction, also acts on a variety of DNA structures
-
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
after introduction of nicks and double-strand scissions liberation of 5'-phosphomononucleotides and 5'-phosphooligonucleotides from the newly created termini
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
higher rate of hydrolysis of alkylated and depurinated dsDNA than native DNA
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
low activity in comparision with ssDNA
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
15-20fold higher activity on single-stranded than native DNA
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
single-strand-specific activity on Bluescript II KS+ supercoiled DNA is inherent property of snake venom exonuclease
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
sequence selective cleavage
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
cleavage of phage PM2 superhelical DNA to relaxed, nicked-circular form and unit length linear form molecules
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
cleavage of phage PM2 superhelical DNA to relaxed, nicked-circular form and unit length linear form molecules
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
only 2% of activity on ssDNA
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
activity on locally altered structures of native DNA like bleomycin-treated DNA, superhelical, depurinated or UV-irradiated DNA
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
activity on locally altered structures of native DNA like bleomycin-treated DNA, superhelical, depurinated or UV-irradiated DNA
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
activity on locally altered structures of native DNA like bleomycin-treated DNA, superhelical, depurinated or UV-irradiated DNA
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
probably exonucleolytic cleavage, acting on linear, but not circular dsDNA
-
?
dsDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
Bacillus subtilis Marburg
-
only 2% of activity on ssDNA
-
?
duplex deoxyadenosine-deoxythymine homopolymer + H2O
5'-dAMP + 5'-TMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
heteroduplex DNA + H2O
nicked DNA
show the reaction diagram
-
cleavage of single base pair mismatches in heteroduplex DNA
-
-
?
platinum-DNA complexes + H2O
5'-phosphomononucleotides + platinum
show the reaction diagram
-
native, denaturated and renaturated platinum-DNA complexes, use of several platinum compounds like diamminedichloroplatinum(II)
-
?
poly(U) + H2O
?
show the reaction diagram
-
preferred substrate
-
-
?
polyA + H2O
fragments of polyA
show the reaction diagram
-
-
-
-
?
polyadenylic acid + H2O
5'-AMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
polyadenylic acid + H2O
5'-AMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
polyadenylic acid + H2O
5'-AMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
polyadenylic acid + H2O
5'-AMP + 5'-phosphooligonucleotides
show the reaction diagram
-
only 5% of activity with denaturated DNA at pH 4.6, 0.05 M NaCl and 45C, at pH 6.4 50% of activity in comparision with DNA
-
?
polycytosylic acid + H2O
5'-CMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
polycytosylic acid + H2O
5'-CMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
polycytosylic acid + H2O
5'-CMP + 5'-phosphooligonucleotides
show the reaction diagram
-
only 5% of activity with denaturated DNA at pH 4.6, 0.05 M NaCl and 45C, at pH 6.4 50% of activity in comparision with DNA
-
?
polycytosylic acid + H2O
5'-CMP + 5'-phosphooligonucleotides
show the reaction diagram
-
only small degradation
-
?
polydeoxyadenylic acid + H2O
5'-dAMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
polydeoxyadenylic acid + H2O
5'-dAMP + 5'-phosphooligonucleotides
show the reaction diagram
-
more slowly, but significant hydrolysis in comparision with denaturated DNA
-
?
polydeoxycytosylic acid + H2O
5'-dCMP + 5'-phosphooligonucleotides
show the reaction diagram
-
only little degradation
-
?
polydeoxyguanylic acid + H2O
5'-dGMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
polydeoxyguanylic acid + H2O
5'-dGMP + 5'-phosphooligonucleotides
show the reaction diagram
-
only little degradation, no degradation in the presence of Ca2+
-
?
polydeoxythymidylic acid + H2O
5'-dTMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
polydeoxythymidylic acid + H2O
5'-dTMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
polyguanylic acid + H2O
5'-GMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
polyguanylic acid + H2O
5'-GMP + 5'-phosphooligonucleotides
show the reaction diagram
-
only small degradation
-
?
polyguanylic acid + H2O
5'-GMP + 5'-phosphooligonucleotides
show the reaction diagram
-
only 5% of activity with denaturated DNA at pH 4.6, 0.05 M NaCl and 45C
-
?
polyinosinic acid + H2O
5'-IMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
polyU + H2O
fragments of polyU
show the reaction diagram
-
-
-
-
?
polyuridylic acid + H2O
5'-UMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
polyuridylic acid + H2O
5'-UMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
polyuridylic acid + H2O
5'-UMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
polyuridylic acid + H2O
5'-UMP + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
polyuridylic acid + H2O
5'-UMP + 5'-phosphooligonucleotides
show the reaction diagram
-
80% of activity with polydeoxythymidylic acid as substrate
-
?
polyuridylic acid + H2O
5'-UMP + 5'-phosphooligonucleotides
show the reaction diagram
-
activity comparable with denaturated DNA at pH 4.6, 0.05 M NaCl and 45C
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
mainly 5'-phosphodi- and trinucleotides, at long incubation time more than 90% 5'-phosphomononucleotides
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
5fold more activity on DNA than RNA
-
?
RNA + H2O
?
show the reaction diagram
-
-
-
-
?
RNA + H2O
fragments of RNA
show the reaction diagram
-
-
-
-
?
RNA + H2O
fragments of RNA
show the reaction diagram
-
-
-
-
?
RNA + H2O
fragments of RNA
show the reaction diagram
-
-
-
-
?
RNA + H2O
5'-mononucleotides
show the reaction diagram
-
AMP-preferential endoexonuclease
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
from 5'-phosphomononucleotides to at least 5'-phosphoheptanucleotides
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
no detectable 5'-phospho mononucleotides, most 5'-phospho tetranucleotides
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
mainly 5'-phosphodi- and trinucleotides, at long incubation time more than 90% 5'-phosphomononucleotides
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
mainly 5'-phosphooligonucleotides with 5-10 residues
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
mouse and guinea-pig satellite DNA
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
heat denaturated, 100C, 5-15 min
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
heat denaturated, 100C, 5-15 min
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
heat denaturated, 100C, 5-15 min
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
heat denaturated, 100C, 5-15 min
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
15 times more active towards thermally denaturated than native DNA
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
no cleavage at single-base mismatches
-
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
specificity for phosphodiester bonds adjoining deoxycytidine
5'-phosphooligonucleotides with average chain lengh of about 50
?
ssDNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
Bacillus subtilis Marburg
-
-
-
?
ssDNA + H2O
fragments of ssDNA
show the reaction diagram
-
-
-
-
?
ssDNA + H2O
fragments of ssDNA
show the reaction diagram
-
-
-
-
?
ssDNA + H2O
fragments of ssDNA
show the reaction diagram
-
-
-
-
?
ssDNA + H2O
fragments of ssDNA
show the reaction diagram
-
-
-
-
?
ssDNA + H2O
fragments of ssDNA
show the reaction diagram
Q9NJY3, Q9NY43
P4 also has exonuclease and phosphomonoesterase activity
-
-
?
tRNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
tRNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
preference for anticodon and 3'-terminal regions
-
?
tRNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
hydrolysis of nonhydrogen-bonded loops
-
?
tRNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
in the anticodon region
-
?
heteroduplex DNA + H2O
nicked DNA
show the reaction diagram
Q9LL59
cleavage of single base pair mismatches in heteroduplex DNA
-
-
?
additional information
?
-
-
T7 endonuclease I is a multifunctional enzyme. The whole enzyme can efficiently resolve four-way junctions and branched DNA produced during phage proliferation. A single catalytic domain of the junction-resolving enzyme T7 endonuclease I is a non-specific nicking endonuclease
-
-
-
additional information
?
-
-
the enzyme is active on DNA and RNA, the enzyme endonucleolytically degrades ssDNA and RNA by 3'-5' mode to produce 5'-oligonucleotides and 5'-mononucleotides. It preferentially degrades poly(U), substrate specificity, overview
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
functional role in recombination and DNA repair in vivo
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
75000 times more active on single-stranded than on native DNA
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
native DNA hydrolysis probably by contaminating nuclease, its inactivation possible at 55C
-
?
DNA + H2O
nicked DNA
show the reaction diagram
-
-
-
-
?
DNA + H2O
nicked DNA
show the reaction diagram
-
resolving of DNA-four way junction
-
-
?
DNA + H2O
nicked DNA
show the reaction diagram
-
resolving of four-way junction
-
-
?
DNA + H2O
nicked DNA
show the reaction diagram
-
T7 endonuclase I resolves four-way junctions by simultaeously introducing two nicks on the two continously stacked strands at sites 5' to the junction, also acts on a variety of DNA structures
-
-
?
DNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
Bacillus subtilis Marburg
-
-
-
?
heteroduplex DNA + H2O
nicked DNA
show the reaction diagram
-
cleavage of single base pair mismatches in heteroduplex DNA
-
-
?
poly(U) + H2O
?
show the reaction diagram
-
preferred substrate
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
-
-
?
RNA + H2O
5'-phosphomononucleotides + 5'-phosphooligonucleotides
show the reaction diagram
-
5fold more activity on DNA than RNA
-
?
RNA + H2O
fragments of RNA
show the reaction diagram
-
-
-
-
?
RNA + H2O
fragments of RNA
show the reaction diagram
-
-
-
-
?
RNA + H2O
fragments of RNA
show the reaction diagram
-
-
-
-
?
ssDNA + H2O
fragments of ssDNA
show the reaction diagram
-
-
-
-
?
ssDNA + H2O
fragments of ssDNA
show the reaction diagram
-
-
-
-
?
ssDNA + H2O
fragments of ssDNA
show the reaction diagram
-
-
-
-
?
ssDNA + H2O
fragments of ssDNA
show the reaction diagram
-
-
-
-
?
heteroduplex DNA + H2O
nicked DNA
show the reaction diagram
Q9LL59
cleavage of single base pair mismatches in heteroduplex DNA
-
-
?
additional information
?
-
-
the enzyme is active on DNA and RNA, the enzyme endonucleolytically degrades ssDNA and RNA by 3'-5' mode to produce 5'-oligonucleotides and 5'-mononucleotides. It preferentially degrades poly(U), substrate specificity, overview
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
2.5fold stimulation of hydrolysis rate of denaturated DNA by 10 mM
Ca2+
-
60% stimulation of activity on denaturated DNA by 5 mM CaCl2
Ca2+
-
can only partially substitute for Zn2+
Ca2+
-
1.7fold stimulation of 3'-AMP hydrolysis with 5 mM CaCl2
Cd2+
-
only marginal stimulating effects
Co2+
-
-
Co2+
-
up to 3fold stimulation of DNA hydrolysis rate by 0.5 mM
Co2+
-
with 1 mM restoration of 72% of activity of 1 mM EDTA-treated enzyme
Co2+
-
at 0.0014 mM 105% restoration of activity after EDTA-inactivation
Co2+
-
optimal concentration 2-10 mM CoCl2
Co2+
-
10 mM, promotes junction cleavage, active site of endonuclease I has binding sites for two metal ions
Co2+
-
can only partially substitute for Zn2+
Fe2+
-
2.5fold stimulation of hydrolysis rate of denaturated DNA by 10 mM
Fe2+
-
10 mM, promotes junction cleavage, active site of endonuclease I has binding sites for two metal ions, strong cleavage results from attack by hydroxyl radicals generated from Fe2+ ions bound into the active site of endonuclease I
Fe2+
-
can only partially substitute for Zn2+
Hg2+
-
maximal activity at 0.01-1 mM at pH 3.5-5.0
KCl
-
required
Mg2+
-
2.5fold stimulation of hydrolysis rate of denaturated DNA by 10 mM
Mg2+
-
hydrolysis of single-stranded DNA in the absence of Mg2+ at 1.5% of the rate at 15 mM Mg2+
Mg2+
-
optimal concentration 1-2 mM MgCl2
Mg2+
-
40% stimulation of activity on denaturated DNA by 5 mM MgCl2
Mg2+
-
less than 1% of maximal activity without Mg2+, maximal activity at 0.5 mM
Mg2+
-
required for resolving of cruciform structures
Mg2+
-
10 mM, promotes junction cleavage, active site of endonuclease I has binding sites for two metal ions
Mg2+
Q9LL59
10 mM, targeting induced local lesions in genomes mismatch cleavage protocol
Mg2+
-
10 mM, cleavage of single base pair mismatches
Mg2+
-
mutant enzymes D55E and E65D show no cleavage of x strand 5'-labeled junction 3 in presence of Mg2+, mutant enzyme E20D shows limited cleavage
Mg2+
-
2fold stimulation of 3'-AMP hydrolysis with 5 mM MgCl2
Mn2+
-
only marginal stimulating effects
Mn2+
-
at 0.0014 mM 53% restoration of activity after EDTA-inactivation
Mn2+
-
required for activity, reduced activity towards ssDNA at 10 mM
Mn2+
-
20times lower activity in resolving of cruciform structures than with Mg2+
Mn2+
-
10 mM, promotes junction cleavage, active site of endonuclease I has binding sites for two metal ions
Mn2+
-
mutant enzyme E65D shoes limited cleavage of x strand 5'-labeled junction 3 in presence of Mn2+
NaCl
-
maximal activity below 50 mM
NaCl
-
required
Zn2+
-
36% activation at 0.1 mM, 16% activation at 1 mM
Zn2+
-
enzyme contains 3 zinc atoms
Zn2+
-
46% stimulation of activity at 0.1 mM Zn2+, maximal stimulation at 0.01 mM
Zn2+
-
metalloprotein with one loosely and two strongly bound zinc atoms; with 1 mM restoration of 76% of activity of 1 mM EDTA-treated enzyme
Zn2+
-
stimulation of activity towards ssDNA at 0.001-0.01 mM, at 0.0014 mM 103% restoration of activity after EDTA-inactivation
Zn2+
-
enzyme contains 3 zinc atoms
Zn2+
-
1 mM, 10fold increase in activity
Zn2+
-
zinc metalloprotein, required for activity
Zn2+
-
2 mM, cleavage of single base pair mismatches
Zn2+
-
0.2 mM, cleavage of single base pair mismatches
Zn2+
-
required
Mn2+
-
can only partially substitute for Zn2+
additional information
-
metalloenzyme
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,10-phenanthroline
-
0.24 mM, complete inhibition of plasmid nicking activity
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
inactivation
2-mercaptoethanol
-
with 4 mM 50% of activity with denaturated DNA
2-mercaptoethanol
-
-
2-mercaptoethanol
-
with 1 mM stabilization of activity at pH 3.5 and 4.0
2-mercaptoethanol
-
-
5'-ATP
-
50% inhibition by 0.5 mM
5'-dAMP
-
50% inhibition by 0.085 mM dAMP
5'-dATP
-
50% inhibition by 0.001 mM dATP
8-hydroxychinoline 5-sulfonate
-
10% of maximal activity with 0.01 mM, no activity with 0.1 mM
-
8-hydroxychinoline 5-sulfonate
-
-
-
8-hydroxychinoline 5-sulfonate
-
8% of maximal activity with 0.01 mM, no activity with 0.1 mM
-
8-hydroxychinoline 5-sulfonate
-
30% of maximal activity with 0.01 mM
-
8-hydroxychinoline 5-sulfonate
-
50% of activity towards ssDNA with 0.005 mM
-
8-hydroxychinoline 5-sulfonate
-
with 1 mM 50% of maximal activity on denaturated DNA, 70% with native DNA
-
amino group modification
-
at pH 8.0, 65-70% loss of initial activity towards ssDNA, RNA and 3'-AMP after modification of amino groups, lysine residues, with 0.5 mM 2,4,6-trinitrobenzenesulfonic acid
-
amino group modification
-
reaction of lysine residues with phthalaldehyde, loss of 80% of initial activity with 0.1 mM
-
aurintricarboxylic acid
-
complete inhibition at 0.005 mM
beta-mercaptoethanol
-
-
Ca2+
-
about 40% inhibition of hydrolysis of RNA
Ca2+
-
30% of activity towards denaturated ssDNA with 1.2 mM
Ca2+
-
35.1% of maximal activity with 0.1 mM denaturated DNA, 74.7% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
Ca2+
-
about 10% of activity towards ssDNA at 1 and 10 mM
Ca2+
-
1 mM, slight inhibition
Ca2+
-
10 mM, complete inhibition of junction cleavage
carboxylate group modification
-
at pH 4.6, 50-85% loss of initial activity towards ssDNA, RNA and 3'-AMP after modification of carboxylate groups with 5-15 mM 1-ethyl-3-(dimethylaminopropyl)-carbodiimide; at pH 7.8, 55-95% loss of initial activity towards ssDNA, RNA and 3'-AMP after modification of carboxylate groups with 2-15 mM Woodward's reagent K
-
citraconylation
-
reversible block of amino groups, lysine residues, by citraconic anhydride
-
citrate
-
at 0.2 M 50% of initial activity with ssDNA
Co2+
-
almost complete inhibition of 3'-AMP hydrolysis by 1 mM CoCl2
CsCl
-
optimal activity 0.5-1.8 M, 27% of maximal activity at 7 M
Cu2+
-
46.0% of maximal activity with 0.1 mM denaturated DNA, 56.0% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Cu2+
-
1 mM, slight inhibition
diethyldicarbonate
-
inactivation
EDTA
-
69% of maximum activity at 20 mM
EDTA
-
95% inhibition at 0.1 mM, reversed by equivalent amounts of Co2+
EDTA
-
no activity after dialysis with 1 mM, no restoration by addition of Zn2+, Co2+, Mg2+, Mn2+, Ca2+ or cysteine
EDTA
-
complete inactiviation at 1 mM, 70% of original activity are restored by addition of 1 mM Zn2+
EDTA
-
95% inhibition at 0.1 mM, reversed by equivalent amounts of Co2+; complete inactiviation at 1 mM, 70% of original activity are restored by addition of 1 mM Zn2+
EDTA
-
complete inactivation by incubation with 20 mM, pH 5.0
EDTA
-
complete inactiviation at 1 mM, 70% of original activity are restored by addition of 1 mM Zn2+; no activity after dialysis with 1 mM, no restoration by addition of Zn2+, Co2+, Mg2+, Mn2+, Ca2+ or cysteine
EDTA
-
almost complete inactivation by 0.01 mM
EDTA
-
15% of maximal activity with 0.01 mM
EDTA
-
almost complete inactivation by 0.01 mM
EDTA
-
55-100% loss of initial activity with 1-10 mM
EDTA
-
50% of activity towards ssDNA with 0.0003 mM
EDTA
-
almost no activity with 2 mM
EDTA
-
1 mM, slight inhibition
EDTA
-
; reversible by Zn2+
EDTA
-
almost complete inhibition of 3'-AMP hydrolysis with 1 mM
EGTA
-
1 mM, slight inhibition
F-
-
complete inhibition by 10 mM
F-
-
18% of maximal activity with 10 mM
F-
-
5% of maximal activity with 10 mM
F-
-
at 0.2 M 30% of initial activity with ssDNA
Fe2+
-
-
-
Fe2+
-
about 40% inhibition of hydrolysis of RNA
-
Fe2+
-
47.7% of maximal activity with 0.1 mM denaturated DNA, almost no activity with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
-
glutathione
-
with 4 mM of the reduced form 50% of activity with denaturated DNA
Hg2+
-
complete inactivation by 5 mM HgCl2
Hg2+
-
31.5% of maximal activity with 0.1 mM denaturated DNA, 48.2% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
Hg2+
-
complete inhibition of 3'-AMP hydrolysis by 1 mM HgCl2
iodoacetamide
-
-
Ionic strength
-
optimal 0.025-0.05 M
-
Ionic strength
-
dependence of tRNA hydrolysis on salt concentration
-
Ionic strength
-
inhibition of activity at ionic strength above 0.3 M
-
Ionic strength
-
about 40% of activity towards ssDNA at 0.2 M NaCl and KCl
-
KCl
-
50% of activity with 0.019 and 0.025 mM
Mg2+
-
about 40% inhibition of hydrolysis of RNA with 10 mM Mg2+
Mg2+
-
concentrations higher 2 mM
Mg2+
-
reduction of activity towards ssDNA at 10 mM
Mg2+
-
1 mM, slight inhibition
MgCl2
-
50 mM, 25% inhibition
N-bromosuccinimide
-
DNA and RNA protects from inactivation
N-ethylmaleimide
-
with 5 mM 67% reduction of hydrolysis of polydeoxythymidylic acid
NaCl
-
inhibition at concentrations higher than 200 mM
NaCl
-
optimal activity at 100 mM, 55% of maximal rate at 400 mM
NaCl
-
maximal activity 0-2 M, 40% of maximal activity at 4,4 M
NaCl
-
maximal activity at 0-100 mM, at 1 mM 71% of maximal activity
NaCl
-
complete inhibition with 50 mM
NaCl
-
inhibition by low concentrations, 50% of activity with 19 and 0.025 mM
NaCl
-
inhibition at concentrations higher than 100 mM
NaCl
-
dependence of cleavage of superhelical, nicked-circular and linear DNA molecules from the NaCl concentration
NaCl
-
inhibition of polydeoxythymidylic acid at 10-50 mM, not reversible by addition of Mg2+
NaCl
-
strong inhibition above 100 mM
NaCl
-
100 mM, 20% inhibition
phosphate
-
50% inhibition by 2 mM sodium phosphate
phosphate
-
-
phosphate
-
80% inhibition by 33 mM potassium phosphate, pH 7.5
phosphate
-
competitive inhibition
phosphate
-
45% of maximal activity with 20 mM potassium phosphate, 26% with 30 mM
Polyvinyl sulfate
-
-
potassium citrate
-
complete inhibition at 10 mM
reductive methylation
-
modification of lysine residues into N,N'-dimethyl lysine
-
sodium bisphosphate
-
50% inhibition by 0.02 mM sodium bisphosphate
sodium bisphosphate
-
at 0.2 M 10% of initial activity with ssDNA
sodium dodecylsulfate
-
above 0.04% decrease of reaction rate. 60% of activity at 0.12%
sodium dodecylsulfate
-
a third of maximal activity at 0.1%, but slight activation below 0.01%
sodium dodecylsulfate
-
up to 0.6% no effect on DNA hydrolysis
sodium dodecylsulfate
-
-
sodium dodecylsulfate
-
complete inactivation with 0.01% at pH 5.0
sodium dodecylsulfate
-
complete inhibition with 0.2%
Urea
-
40% of maximal activity with single-stranded DNA with 6.6 M
Zn2+
-
54% of initial activity towards ssDNA with 0.1 mM Zn2+
Zn2+
-
54% of initial activity towards ssDNA with 0.1 mM Zn2+; almost complete inactivation at 1.0 mM
Zn2+
-
33.7% of maximal activity with 0.1 mM denaturated DNA, 65.2% with 0.5 mM polydeoxythymidylic acid as substrate, noncompetitive inhibition
Zn2+
-
about 15% of activity towards ssDNA at 1 and 10 mM
Mn2+
-
6.5% of maximal activity with 0.1 mM denaturated DNA, 8.4% with 0.5 mM polydeoxythymidylic acid as substrate, competitive inhibition
additional information
-
urea does not influence the enzyme at any concentrations
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
dimethyl sulfoxide
-
-
glutathione
-
oxidized form, about 2fold stimulation of activity at all pH
glycerol
-
2fold stimulation of activity by 10-20% in 50 mM Tris-HCl-buffer, pH 7.5
NaCl
-
optimal activity at 100 mM
Triton X-100
-
-
Triton X-100
-
stabilization of activity at pH 5.0 with 0.001%
Triton X-100
-
10fold stimulation of activity with 0.3% v/v
Trypsin
-
almost 2.5fold stimulation of activity, probably due to a conversion into a smaller enzyme form
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.04
-
3'-AMP
-
-
37.6
-
3'-AMP
-
-
71.2
-
3'-CMP
-
-
470
-
3'-dCMP
-
-
223
-
3'-dGMP
-
-
608
-
3'-dTMP
-
-
47.1
-
3'-GMP
-
-
79.3
-
3'-UMP
-
-
3.4
-
adenosine 3',5'-bisphosphate
-
-
0.02
-
denaturated DNA
-
-
-
0.7
-
denaturated DNA
-
-
-
69.9
-
deoxythymidine 3',5'-bisphosphate
-
-
0.02
-
polydeoxythymidylic acid
-
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.5
-
5'-ATP
-
-
0.085
-
5'-dAMP
-
-
0.001
-
5'-dATP
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.01
-
-
purified enzyme, polydeoxythymidylic acid as substrate
0.63
-
-
with 3'-dAMP as substrate
1.06
-
-
with 2'-AMP as substrate
2.39
-
-
with 3'-dGMP as substrate
3.76
-
-
with 3'dCMP as substrate
4.83
-
-
with 3'-dTMP as substrate
7.7
-
-
mitochondrial activity with ssDNA as substrate
8.3
-
-
purified enzyme, DNA as substrate
12.7
-
-
purified enzyme, heat-denaturated DNA as substrate
17.2
-
-
with deoxythymidine 3',5'-bisphosphate as substrate
241
-
-
with 3'-GMP as substrate
242
-
-
with adenosine 3',5'-bisphosphate as substrate
248
-
-
with 3'-AMP as substrate
255
-
-
with 3'-UMP as substrate
266
-
-
with 3'-CMP as substrate
810
-
-
with heat-denaturated DNA at pH 8.0 in the presence of 10 mM Mg2+
41250
-
-
purified enzyme, pH 3.7, 75C
additional information
-
-
0.6-0.8 U/mg, 1 unit is defined as the amount of enzmyme that is needed to convert 0.001 mg of supercoiled pUC(AT) to either the linear or nicked form in a 0.02 ml reaction at 37C in 30 min
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3.8
4.3
-
pH 3.8 for the immobilized, pH 4.3 for the soluble enzyme
4.5
5
-
with denaturated DNA in the presence of 1 mM Zn2+
4.5
-
-
in 10 mM NaCl
4.6
-
-
DNA cleavage assay
4.6
-
-
DNA digestion
4.6
-
-
DNA hydrolysis assay
5
-
-
because of rapid inactivation, stabilization by 0.1 mM Zn2+ or 1 mM cysteine, glutathione or dithiothreitol recommended
5
-
-
with natural single-stranded DNA and RNA as substrate
5.5
-
-
-
5.5
-
-
cleavage of single base pair mismatches
6
7
-
with 3'-AMP and adenosine 3',5'-biphosphate as substrate
6.5
-
-
cleavage of single base pair mismatches
7.2
7.6
-
with DNA and RNA as substrate
7.5
8.5
-
0.1-0.2 M Tris-HCl buffer
7.5
-
-
with 10 mM sodium phosphate buffer
7.5
-
-
-
7.5
-
-
in Tris-HCl-buffer, at this pH only 30% of this activity in potassium phosphate buffer, only 60% in sodium citrate buffer
7.5
-
Q9LL59
targeting induced local lesions in genomes mismatch cleavage protocol
8
-
-
optimal in 0.1 M NaCl
9.2
-
-
-
9.2
-
-
with denaturated DNA and polydeoxythymidylic acid as substrate
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2.5
8
-
activity range
3
8
-
almost 50% of activity at pH 3.0 and 5.5
3
8.5
-
with mononucleotide substrates
3.1
6.4
-
almost 50% of activity at pH 3.3 and 4.6
4
6
-
with synthetic homopolynucleotides as substrate
4.5
10.5
-
12.3% of maximal activity at pH 4.5, 43.3% at pH 10.5
5.8
9.3
-
50% of activity at pH 5.8 and 9.3
6.5
7.5
-
50% of optimal activity towards ssDNA and dsDNA
6.5
8
-
-
6.5
8.5
-
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
-
37
-
-
DNA cleavage assay
37
-
-
DNA hydrolysis assay
45
-
-
with denaturated DNA as substrate
45
-
Q9LL59
targeting induced local lesions in genomes mismatch cleavage protocol
45
-
-
cleavage of single base pair mismatches
45
-
-
DNA digestion
55
-
-
soluble enzyme
55
-
-
soluble and immobilized enzyme
60
-
-
with native DNA or 3'-AMP as substrate
60
-
-
cleavage of single base pair mismatches
60
-
-
with native DNA or 3'-AMP as substrate
65
-
-
immobilized enzyme
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
50
-
with tRNA as substrate
20
80
-
-
30
60
-
optimal temperature for denaturated DNA 60C, 13% of maximal activity at 30C
47
62
-
maximal hydrolysis rate of denaturated DNA and RNA
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
9.5
-
-
deduced from nucleotide sequence
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20000
-
-
gel filtration
32000
38000
-
35000: enzyme from nonsenescent flag leaves, 38000: from senescent leaves
32000
-
-
SDS-PAGE
32000
-
-
SDS-PAGE
32000
-
-
gel filtration
32000
-
-
SDS-PAGE
34000
-
-
gel filtration
35000
-
-
gel filtration
36000
38000
-
SDS-PAGE
36000
-
-
gel electrophoresis
37400
-
-
SDS-PAGE
38000
-
-
SDS-PAGE
39000
-
-
reduction with 2-mercaptoethanol prior to SDS-PAGE generates two polypeptides of 25000 and 15000, linked covalently by disulfide bond, intact and cleaved forms are equally active
39000
-
-
reduction with 2-mercaptoethanol prior to SDS-PAGE generates two polypeptides of 25000 and 15000, linked covalently by disulfide bond, intact and cleaved forms are equally active
42000
50000
-
-
42000
-
-
gel filtration
42000
-
-
gel filtration
43000
-
-
sucrose density gradient sedimentation
43000
-
-
gel filtration
44000
-
-
gel filtration
45000
-
-
SDS-PAGE
47000
-
-
gel filtration
54000
-
-
-
54000
-
-
SDS-PAGE
54000
-
-
native enzyme
60000
-
-
SDS-PAGE
76000
-
-
-
90000
-
-
single inactive precursor polypeptide, generation of four nucleases by proteolysis: 75000: Mg2+ dependent exonuclease, 65000: endo-exonuclease, 55000: single-strand-specific endonuclease, 65000: Dnase A, a Ca2+ dependent endonuclease
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 26000, SDS-PAGE, probaly a dimer
?
-
x * 32000
?
Q9NJY3, Q9NY43
x * 35000, SDS-PAGE, precursor
dimer
-
alpha,beta, 1 * 30200 + 1 * 24000, SDS-PAGE
monomer
-
1 * 35000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-
large number of mannose residues
glycoprotein
-
18% carbohydrates
glycoprotein
-
17.4% carbohydrate consisting of mannose, galactose, glucosamine in a ratio of 6/2/1
glycoprotein
-
about 20% carbohydrate
glycoprotein
-
29% carbohydrate by weight
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
crystal structure at 2.1 A resolution
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3.6
5.2
-
comparatively higher stability of the immobilized than the soluble enzyme
4.8
5.5
-
Zn2+ essential for stabilizing activity
5.5
9
-
in 30 mM buffer with 200 mM NaCl, more stable above pH 7.0 than below
7
8
-
stable without additions
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
70
-
at 70C almost 75% of initial activity with immobilized, 20% with soluble enzyme
30
70
-
50% of activity with ssDNA at 47C, with dsDNA at 49C
50
-
-
40% loss of activity with polyuridylic acid or polydeoxythymidylic acid as substrate after heating of the enzyme fraction for 5 min
55
65
-
no inactivation by heating at 55C, partial inactivation at 65C, importance of high content of hydrophobic amino acids and zinc atoms for thermostability postulated
60
67
-
stable below 60C, 50% inactivation after heating at 67C for 15 min, pH 6.0
60
70
-
stable to heating at pH 5.0 in the presence of both Zn2+ and sulfhydryl compounds
60
80
-
at 70C complete loss of activity of the soluble enzyme, 40% of maximal activity of the immobilized enzyme at this temperature, 20% at 80C
60
-
-
after 90 min incubation time 90% of activity towards 3'-AMP and 60% of activity towards DNA lost
65
-
-
no inactivation of ribonuclease and deoxyribonuclease activity by heating at this temperature in the presence of substrate
74
-
-
in 0.02 M potassium phosphate buffer, pH 6.5, 37% of activity towards dsDNA after heating of 2 min, no activity after heating of 10 min; in 0.02 M potassium phosphate buffer, pH 6.5, 44% of activity towards ssDNA after heating of 2 min, 4% after heating of 10 min
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
no loss of activity in the presence of 100-250 mM glyoxal
-
stable to high concentrations of urea, susceptible to low concentrations of SDS and guanidine-HCl
-
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimethyl sulfoxide
-
no loss of activity in 50% dimethylsulfoxide
dimethylformamide
-
no loss of activity in 30% dimethylformamide
formaldehyde
-
no loss of activity in 2% formaldehyde
formamide
-
no loss of activity in 60% formamide
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, purified enzyme after dialysis against deionized water, no loss of activity
-
4C, 0.1 M buffer pH 3.5-4.0, mercaptoethanol, 22 h, 16.9% of initial activity at pH 3.5, 59% at pH 4.0, by preincubation with Zn2+ 48% of initial activity at pH 3.5, 86% at pH 4.0
-
4C, 30 mM sodium acetate buffer pH 4.6, containing 1 mM ZnSO4, 50 mM NaCl and 5% glycerol, more than 45 days, no apparent loss of activity
-
4C, crude and purified form, no loss of activity after several years
-
-20C, 50% glycerol v/v, 6 months, no loss of activity of the purified enzyme
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recycling isoelectric focusing
-
recombinant endonuclease I
-
recombinant T7 endonuclease
-
recombinant T7 endonuclease I
-
affinity purification
Q9NJY3, Q9NY43
recombinant carboxy-terminal hexahistidine tag fusion protein
-
from mitochondria
-
heparin-Sepharose, partially purified
-
extracellular enzyme 165fold by chromatography with modified chitosan
-
from nucleoplasm
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
-
expression in Escherichia coli
-
expression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D55E
-
no cleavage of x strand 5'-labeled junction 3 in presence of Mg2+
D55N
-
similar binding affinity for DNA as wild-type, approx. 0.02% of wild-type DNA cleavage rate
D55N
-
3fold higher DNA-junction cleavage rate as wild-type
D74A
-
lower binding affinity for DNA as wild-type, approx. 15% of wild-type DNA cleavage rate
D74N
-
lower binding affinity for DNA as wild-type, approx. 30% of wild-type DNA cleavage rate
E20A
-
lower binding affinity for DNA as wild-type, approx. 0.5% of wild-type DNA cleavage rate
E20A
-
3fold higher DNA-junction cleavage rate as wild-type
E20D
-
limited cleavage of x strand 5'-labeled junction 3 in presence of Mg2+
E20Q
-
3fold lower binding affinity for DNA as wild-type, approx. 0.1% of wild-type DNA cleavage rate
E20Q
-
similar DNA-junction cleavage rate as wild-type
E35K
-
lower binding affinity for DNA as wild-type, slightly lower rate of DNA cleavage
E65D
-
no cleavage of x strand 5'-labeled junction 3 in presence of Mg2+, limited activity is restored in presence of Mn2+
E65K
-
similar binding affinity for DNA as wild-type, approx. 0.02% of wild-type DNA cleavage rate
E65K
-
2fold higher DNA-junction cleavage rate as wild-type
E65Q
-
slightly higher binding affinity for DNA as wild-type, approx. 1% of wild-type DNA cleavage rate
E65Q
-
similar DNA-junction cleavage rate as wild-type
K67A
-
lower binding affinity for DNA as wild-type, approx. 0.3% of wild-type DNA cleavage rate
K67A
-
2fold higher DNA-junction cleavage rate as wild-type
K67R
-
7fold lower binding affinity for DNA as wild-type, approx. 0.01% of wild-type DNA cleavage rate
P54L
-
lower binding affinity for DNA as wild-type, approx. 0.5% of wild-type DNA cleavage rate
P54L
-
3fold higher DNA-junction cleavage rate as wild-type
K67R
-
similar DNA-junction cleavage rate as wild-type
additional information
-
mutation in beta-bridge center that separates the catalytic domains, 20-50 times lower nonspecific nuclease activity in Mg2+ buffer
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
analysis
-
analysis of heteroduplex and superhelical DNA, quantitation of nucleic acid hybridization
analysis
-
analysis of tRNA for determination of amino acid specifity
analysis
-
in DNA-RNA annaeling experiments as reagent for selective removal of nonhybridized polynucleotides
analysis
-
-
analysis
-
studies of nucleic acid secondary structure
analysis
-
reagent for mapping splice points and ordered regions in RNA; studies of nucleic acid secondary structure
analysis
-
reagent for mapping splice points and ordered regions in RNA
analysis
-
used in S1 nuclease protection assays
molecular biology
-
straightforward detection of single-nucleotide polymorphisms in double-stranded DNA by using exonuclease III/nuclease S1/peptide nucleic acid system
synthesis
-
industrial production of 5'-phosphomononucleotides from yeast RNA
synthesis
-
removal of DNA loops during cDNA synthesis
molecular biology
-
mutation detection in DNA oligonucleotides based on a guanine-quenching method coupled with T7 endonuclease-catalysed digestion of single-stranded DNA
analysis
-
gene isolation
analysis
-
analysis of cap structures at the 5'-termini of eucaryotic mRNA
synthesis
-
industrial production of 5'-phosphomononucleotides from yeast RNA
analysis
-
studies of nucleic acid secondary structure