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Literature summary for 3.1.30.1 extracted from

  • Vogt, V.M.
    Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae (1973), Eur. J. Biochem., 33, 192-200.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
NaCl optimal activity at 100 mM, 55% of maximal rate at 400 mM Aspergillus oryzae
sodium dodecylsulfate above 0.04% decrease of reaction rate. 60% of activity at 0.12% Aspergillus oryzae

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ only marginal stimulating effects Aspergillus oryzae
Co2+
-
Aspergillus oryzae
Mn2+ only marginal stimulating effects Aspergillus oryzae
Zn2+
-
Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
SDS-PAGE Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Aspergillus oryzae
-
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
RNA + H2O Aspergillus oryzae
-
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
polyadenylic acid + H2O
-
Aspergillus oryzae 5'-AMP + 5'-phosphooligonucleotides
-
?
polycytosylic acid + H2O
-
Aspergillus oryzae 5'-CMP + 5'-phosphooligonucleotides
-
?
polyguanylic acid + H2O
-
Aspergillus oryzae 5'-GMP + 5'-phosphooligonucleotides
-
?
polyuridylic acid + H2O
-
Aspergillus oryzae 5'-UMP + 5'-phosphooligonucleotides
-
?
RNA + H2O
-
Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
ssDNA + H2O heat denaturated, 100°C, 5-15 min Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?

Synonyms

Synonyms Comment Organism
single-strand-specific nuclease
-
Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.3
-
Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
3.1 6.4 almost 50% of activity at pH 3.3 and 4.6 Aspergillus oryzae