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Literature summary for 3.1.30.1 extracted from
- A nuclease specific for heat denaturated DNA isolated from a product of Aspergillus oryzae (1966), Biochim. Biophys. Acta, 114, 158-168.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 69% of maximum activity at 20 mM | Aspergillus oryzae |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 36% activation at 0.1 mM, 16% activation at 1 mM | Aspergillus oryzae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | Aspergillus oryzae | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bis(p-nitrophenyl)phosphate + H2O | almost no hydrolysis | Aspergillus oryzae | p-nitrophenol + phosphate | - |
? | |
| DNA + H2O | - |
Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| ssDNA + H2O | heat denaturated, 100°C, 5-15 min | Aspergillus oryzae | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | 4.6 | - |
Aspergillus oryzae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | almost 50% of activity at pH 3.0 and 5.5 | Aspergillus oryzae |
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Release 2023.1 (January 2023)
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