Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxychinoline 5-sulfonate | 50% of activity towards ssDNA with 0.005 mM | Schizophyllum commune | |
citrate | at 0.2 M 50% of initial activity with ssDNA | Schizophyllum commune | |
EDTA | 50% of activity towards ssDNA with 0.0003 mM | Schizophyllum commune | |
F- | at 0.2 M 30% of initial activity with ssDNA | Schizophyllum commune | |
heparin | - |
Schizophyllum commune | |
Ionic strength | inhibition of activity at ionic strength above 0.3 M | Schizophyllum commune | |
phosphate | - |
Schizophyllum commune | |
Polyvinyl sulfate | - |
Schizophyllum commune | |
sodium bisphosphate | at 0.2 M 10% of initial activity with ssDNA | Schizophyllum commune | |
Zn2+ | 54% of initial activity towards ssDNA with 0.1 mM Zn2+; almost complete inactivation at 1.0 mM | Schizophyllum commune |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | at 0.0014 mM 105% restoration of activity after EDTA-inactivation | Schizophyllum commune | |
Mn2+ | at 0.0014 mM 53% restoration of activity after EDTA-inactivation | Schizophyllum commune | |
Zn2+ | stimulation of activity towards ssDNA at 0.001-0.01 mM, at 0.0014 mM 103% restoration of activity after EDTA-inactivation | Schizophyllum commune |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
- |
Schizophyllum commune |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Schizophyllum commune | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
RNA + H2O | Schizophyllum commune | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizophyllum commune | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Schizophyllum commune |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Schizophyllum commune | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
dsDNA + H2O | - |
Schizophyllum commune | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
RNA + H2O | - |
Schizophyllum commune | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
ssDNA + H2O | - |
Schizophyllum commune | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SC nuclease | - |
Schizophyllum commune |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Schizophyllum commune |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 50% of activity with ssDNA at 47°C, with dsDNA at 49°C | Schizophyllum commune |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | 50% of optimal activity towards ssDNA and dsDNA | Schizophyllum commune |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | in 30 mM buffer with 200 mM NaCl, more stable above pH 7.0 than below | Schizophyllum commune |