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Literature summary for 3.1.30.1 extracted from

  • Laskowski, M.
    Purification and properties of the mung bean nuclease (1980), Methods Enzymol., 65, 263-276.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
L-serine
-
Vigna radiata var. radiata

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ at 1 mM Vigna radiata var. radiata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
reduction with 2-mercaptoethanol prior to SDS-PAGE generates two polypeptides of 25000 and 15000, linked covalently by disulfide bond, intact and cleaved forms are equally active Vigna radiata var. radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Vigna radiata var. radiata
-
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?

Organism

Organism UniProt Comment Textmining
Vigna radiata var. radiata
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 29% carbohydrate by weight Vigna radiata var. radiata

Purification (Commentary)

Purification (Comment) Organism
-
Vigna radiata var. radiata

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol v/v, 6 months, no loss of activity of the purified enzyme Vigna radiata var. radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-AMP + H2O
-
Vigna radiata var. radiata adenosine + phosphate
-
?
DNA + H2O
-
Vigna radiata var. radiata 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
dsDNA + H2O
-
Vigna radiata var. radiata 5'-phosphomononucleotides + 5'-phosphooligonucleotides after introduction of nicks and double-strand scissions liberation of 5'-phosphomononucleotides and 5'-phosphooligonucleotides from the newly created termini ?
ssDNA + H2O
-
Vigna radiata var. radiata 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?

Synonyms

Synonyms Comment Organism
mung bean nuclease
-
Vigna radiata var. radiata
mung bean nuclease I
-
Vigna radiata var. radiata

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8 stable without additions Vigna radiata var. radiata