Information on EC 3.1.1.74 - cutinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.1.1.74
-
RECOMMENDED NAME
GeneOntology No.
cutinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cutin + H2O = cutin monomers
show the reaction diagram
Cutin, a polymeric structural component of plant cuticles, is a hydroxy fatty acid polymer, usually C16 or C18 and that contains one to three hydroxyl groups. The enzyme from several fungal sources also hydrolyses the p-nitrophenyl esters of hexadecanoic acid. It is however inactive towards several esters that are substrates for non-specific esterases.
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic ester hydrolysis
hydrolysis
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SYSTEMATIC NAME
IUBMB Comments
cutin hydrolase
Cutin, a polymeric structural component of plant cuticles, is a polymer of hydroxy fatty acids that are usually C16 or C18 and contain up to three hydroxy groups. The enzyme from several fungal sources also hydrolyses the p-nitrophenyl esters of hexadecanoic acid. It is however inactive towards several esters that are substrates for non-specific esterases.
CAS REGISTRY NUMBER
COMMENTARY hide
51377-41-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain 15
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Manually annotated by BRENDA team
strain 20
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Manually annotated by BRENDA team
strain 211
-
-
Manually annotated by BRENDA team
strain 35
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Manually annotated by BRENDA team
strain CECT
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Manually annotated by BRENDA team
gene anig5
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Botrytis cinerea Pser.: Fr.
Pser.: Fr.
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
the organism harbors one cutinase
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
cutinase-like enzyme
UniProt
Manually annotated by BRENDA team
majus
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-
Manually annotated by BRENDA team
Fusarium roseum
Cut1; gene cut1, the organism harbors two cutinases
UniProt
Manually annotated by BRENDA team
Helminthosporium sativum
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-
-
Manually annotated by BRENDA team
Humilica insolens
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
no activity in Saccharomycotina
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Manually annotated by BRENDA team
the organism harbors one cutinase
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Manually annotated by BRENDA team
Pseudozyma antarctica
gene PANT_9c00247; gene PANT_9c00247
UniProt
Manually annotated by BRENDA team
Cut1; gene cut1, the organism harbors one cutinase
UniProt
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
gene SsCut
Uniprot
Manually annotated by BRENDA team
gene ScCut1
UniProt
Manually annotated by BRENDA team
gene ScCut1
UniProt
Manually annotated by BRENDA team
Cut1; gene cut1, the organism harbors two cutinases
UniProt
Manually annotated by BRENDA team
Thc_Cut1 and Thc_Cut2
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-
Manually annotated by BRENDA team
Cut1
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-
Manually annotated by BRENDA team
Thf42_Cut1
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-
Manually annotated by BRENDA team
T34
UniProt
Manually annotated by BRENDA team
T34
UniProt
Manually annotated by BRENDA team
metagenome-derived LC-cutinase
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,4-butanediol + adipic acid
?
show the reaction diagram
-
polycondensation reaction, degree of polymerization is 13
-
-
?
1,4-cyclohexanedimethanol + adipic acid
?
show the reaction diagram
-
polycondensation reaction, degree of polymerization is 16
-
-
?
1,4-cyclohexanedimethanol + sebacic acid
?
show the reaction diagram
-
polycondensation reaction, degree of polymerization is 61
-
-
?
1,4-cyclohexanedimethanol + suberic acid
?
show the reaction diagram
-
polycondensation reaction, degree of polymerization is 18
-
-
?
1,4-cyclohexanedimethanol + succinic acid
?
show the reaction diagram
-
polycondensation reaction, degree of polymerization is 4
-
-
?
1,8-octanediol + adipic acid
?
show the reaction diagram
-
polycondensation reaction, degree of polymerization is 47
-
-
?
2-hydroxyethyl benzoate + H2O
ethane-1,2-diol + benzoate
show the reaction diagram
4-nitrophenyl (16-methyl sulfone ester) hexadecanoate + H2O
?
show the reaction diagram
-
-
-
?
4-nitrophenyl (16-methyl sulfonyl ester) hexadecanoate + H2O
4-nitrophenol + (16-methyl sulfonyl ester) hexadecanoate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl (16-methyl sulfonyl ester) hexadecanoate + H2O
4-nitrophenol + 16-(methylsulfonyl)hexadecanoate
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate
4-nitrophenol + acetate
show the reaction diagram
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
4-nitrophenyl butanoate + H2O
4-nitrophenol + butanoate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl butyrate
4-nitrophenol + butyrate
show the reaction diagram
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
show the reaction diagram
4-nitrophenyl caprylate + H2O
4-nitrophenol + caprylate
show the reaction diagram
4-nitrophenyl decanoate + H2O
4-nitrophenol + decanoate
show the reaction diagram
4-nitrophenyl dodecanoate + H2O
4-nitrophenol + dodecanoate
show the reaction diagram
4-nitrophenyl hexanoate
4-nitrophenol + hexanoate
show the reaction diagram
4-nitrophenyl hexanoate + H2O
4-nitrophenol + hexanoate
show the reaction diagram
4-nitrophenyl laurate + H2O
4-nitrophenol + laurate
show the reaction diagram
4-nitrophenyl myristate + H2O
4-nitrophenol + myristate
show the reaction diagram
4-nitrophenyl octanoate + H2O
4-nitrophenol + octanoate
show the reaction diagram
4-nitrophenyl palmitate + H2O
4-nitrophenol + palmitate
show the reaction diagram
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl propionate + H2O
4-nitrophenol + propionate
show the reaction diagram
4-nitrophenyl valerate
4-nitrophenol + pentanoate
show the reaction diagram
4-nitrophenyl valerate + H2O
4-nitrophenol + pentanoate
show the reaction diagram
4-nitrophenyl valerate + H2O
4-nitrophenol + valerate
show the reaction diagram
4-nitrophenylbutyrate + H2O
4-nitrophenol + butyrate
show the reaction diagram
-
-
-
-
?
6-mercapto-1-butanol + methyl acrylate
6-mercaptobutyl acrylic ester
show the reaction diagram
-
besides two minor Michael-addition by-products, 6-mercaptobutyl acrylic ester is identified as the main product with the thiol as the functional end group
-
-
?
6-mercapto-1-butanol + methyl methacrylate
6-mercaptobutyl methacrylic ester
show the reaction diagram
-
-
-
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?
6-mercapto-1-hexanol + methyl acrylate
6-mercaptohexyl acrylic ester
show the reaction diagram
-
besides two minor Michael-addition by-products, 6-mercaptohexyl acrylic ester is identified as the main product with the thiol as the functional end group
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?
6-mercapto-1-hexanol + methyl methacrylate
6-mercaptohexyl methacrylic ester
show the reaction diagram
-
-
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-
?
6-mercapto-1-hexanol + methyl propionate
6-mercaptohexyl propionic ester
show the reaction diagram
-
-
-
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?
beta-butyrolactone
?
show the reaction diagram
-
ring-opening polymerizations
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-
?
birch bark suberin + H2O
?
show the reaction diagram
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-
-
?
bis(2-hydroxyethyl)terephthalate + H2O
?
show the reaction diagram
bis(benzoyloxyethyl)terephthalate + H2O
terephthalate + benzoic acid + 2-hydroxyethylbenzoate + mono-(2-hydroxyethyl)terephthalate + bis-(2-hydroxyethyl)terephthalate
show the reaction diagram
bisbenzoyloxyethyl terephthalate + H2O
terephthalic acid + mono(2-hydroxyethyl) terephthalate + bis(2-hydroxyethyl)terephthalate + benzoic acid + 2-hydroxyethyl benzoate
show the reaction diagram
cutin + H2O
16-hydroxyhexadecanoic acid + 10,16-dihydroxyhexadecanoic acid + 9,10,18-trihydroxyoctadecanoic acid + 9,10,18-trihydroxyoctadecanoic acid-9.enoic acid
show the reaction diagram
cutin + H2O
cutin monomers
show the reaction diagram
cyclohexyl hexadecanoate + H2O
hexadecanoic acid + cyclohexanol
show the reaction diagram
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-
-
-
?
delta-valerolactone
?
show the reaction diagram
-
ring-opening polymerizations
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?
dihexyl phthalate + H2O
1,3-isobenzofurandione + ?
show the reaction diagram
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-
-
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?
dihexylphthalate + H2O
?
show the reaction diagram
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degradation by cutinase is nearly 70% after 4 h, while 85% of the initial amount remains intact after 72 h of incubation with Candida cylindracea esterase. Products of cutinase-catalyzed hydrolysis are less toxic than those employing Candida cylindracea esterase
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?
dipentyl phthalate + H2O
?
show the reaction diagram
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degradation rate of fungal cutinase is high, i.e., almost 60% of the initial dipentyl phthalate is decomposed within 2.5 hours, and nearly 40% of the degraded dipentyl phthalate disappears within the initial 15 min
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?
dipropyl phthalate + H2O
1,3-isobenzofurandione + propanol
show the reaction diagram
-
-
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?
epsilon-caprolactone
?
show the reaction diagram
-
ring-opening polymerizations
-
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?
ethyl butyrate + H2O
butyric acid + ethanol
show the reaction diagram
-
-
-
?
ethyl caprylate + H2O
caprylic acid + ethanol
show the reaction diagram
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-
-
?
hexadecyl hexadecanoate + H2O
hexadecanoic acid + hexadecanol
show the reaction diagram
-
weak activity
-
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?
malathion + H2O
?
show the reaction diagram
-
-
-
-
?
malathion + H2O
malathion monoacid + malathion diacid + ethanol
show the reaction diagram
-
60% of initial 500 mg/l malathion are degraded within 0.5 h
diacid is the major degradation product
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?
methyl acetate + H2O
acetic acid + methanol
show the reaction diagram
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-
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?
methyl butyrate + H2O
butyric acid + methanol
show the reaction diagram
-
-
-
?
methyl caproate + H2O
caproic acid + methanol
show the reaction diagram
-
-
-
?
methyl caprylate + H2O
caprylic acid + methanol
show the reaction diagram
-
-
-
?
methyl decanoate + H2O
decanoic acid + methanol
show the reaction diagram
-
-
-
?
methyl hexadecanoate + H2O
hexadecanoic acid + methanol
show the reaction diagram
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-
-
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?
methyl laurate + H2O
lauric acid + methanol
show the reaction diagram
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-
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?
methyl myristate + H2O
myristic acid + methanol
show the reaction diagram
-
-
-
?
methyl propionate + H2O
propionic acid + methanol
show the reaction diagram
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-
-
?
n-butyl benzyl phthalate + H2O
1,3-isobenzofurandione + n-butanol + benzyl alcohol
show the reaction diagram
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-
major product, less than 5% of byproducts such as dimethyl phthalate, butyl methyl phthalate
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?
omega-pentadecalactone
?
show the reaction diagram
-
ring-opening polymerizations
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?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
show the reaction diagram
p-nitrophenyl butyrate + H2O
p-nitrophenol + butyrate
show the reaction diagram
p-nitrophenyl butyrate + H2O
phenol + butyrate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl caprate + H2O
p-nitrophenol + ?
show the reaction diagram
concentration of substrate dispersion is 5 mM
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-
?
p-nitrophenyl caproate + H2O
p-nitrophenol + ?
show the reaction diagram
concentration of substrate dispersion is 5 mM
-
-
?
p-nitrophenyl hexanoate + H2O
p-nitrophenol + hexanoate
show the reaction diagram
p-nitrophenyl laurate + H2O
p-nitrophenol + ?
show the reaction diagram
concentration of substrate dispersion is 5 mM
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-
?
p-nitrophenyl myristate + H2O
p-nitrophenol + ?
show the reaction diagram
concentration of substrate dispersion is 5 mM
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?
p-nitrophenyl palmitate + H2O
p-nitrophenol + ?
show the reaction diagram
concentration of substrate dispersion is 5 mM
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-
?
p-nitrophenyl propionate + H2O
p-nitrophenol + ?
show the reaction diagram
concentration of substrate dispersion is 5 mM
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-
?
p-nitrophenyl stearate + H2O
p-nitrophenol + ?
show the reaction diagram
a lower concentration of p-nitrophenyl stearate (2.5 mM) is used due to its lower solubility
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-
?
p-nitrophenyl valerate + H2O
p-nitrophenol + pentanoate
show the reaction diagram
p-nitrophenylbutanoate + H2O
p-nitrophenol + butanoate
show the reaction diagram
p-nitrophenyldecanoate + H2O
p-nitrophenol + decanoate
show the reaction diagram
p-nitrophenylhexadecanoate + H2O
p-nitrophenol + hexadecanoate
show the reaction diagram
-
-
-
-
?
p-nitrophenyltetradecanoate + H2O
p-nitrophenol + tetradecanoate
show the reaction diagram
-
-
-
-
?
poly(caprolactone) + H2O
?
show the reaction diagram
poly(ethylene terephthalate) + H2O
mono-(2-hydroxyethyl) terephthalate + terephthalic acid
show the reaction diagram
polyamide + H2O
?
show the reaction diagram
-
-
-
-
?
polyethylene terephthalate + H2O
terephthalate + ?
show the reaction diagram
-
-
-
-
?
polyethyleneterephthalate + H2O
terephthalate + benzoic acid + 2-hydroxyethylbenzoate + mono-(2-hydroxyethyl)terephthalate + bis-(2-hydroxyethyl)terephthalate
show the reaction diagram
suberin + H2O
?
show the reaction diagram
9,10-epoxy-18-hydroxy 5 octadecanoic acid
-
-
?
tributyrin + H2O
?
show the reaction diagram
tributyrin + H2O
butyric acid + 1,2-dibutyrylglycerol
show the reaction diagram
-
-
-
?
tricaprylin + H2O
?
show the reaction diagram
triglyceride + H2O
?
show the reaction diagram
-
triglycerides in which one of the primary acyl ester functions has been replaced by an alkyl grpup and the secondary acyl ester bond has been replaced by an acyl amino bond. The activity is very sensitive to the length and distribution of the acyl chains, the highest activity is found when the chains at position 1 and 3 contain three or four carbon atoms
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-
?
trioctanoin + H2O
?
show the reaction diagram
-
-
-
?
triolein + H2O
?
show the reaction diagram
tripalmitin + H2O
?
show the reaction diagram
-
-
-
?
tristearin + H2O
?
show the reaction diagram
-
-
-
?
tritiated apple cutin + H2O
?
show the reaction diagram
-
-
-
?
cutin + H2O
additional information
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cutin + H2O
16-hydroxyhexadecanoic acid + 10,16-dihydroxyhexadecanoic acid + 9,10,18-trihydroxyoctadecanoic acid + 9,10,18-trihydroxyoctadecanoic acid-9.enoic acid
show the reaction diagram
cutin + H2O
cutin monomers
show the reaction diagram
poly(caprolactone) + H2O
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
activates isozyme Tfu 0883 7% at 1 mM
K+
-
activates at 1 mM
KCl
-
activates 12% at 1 mM
NaCl
-
activates 15% at 1 mM; activates 20% at 1 mM
Ni2+
-
activates isozyme Tfu 0882 5% and isozyme Tfu 0883 7% at 1 mM
NiCl2
-
activates 11% at 1 mM
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(O,O)-diethyl-(3,5,6-trichloro)-2-pyridylphosphorothioate
-
1 mM, 90% inhibition
1-Heptanol
-
27% inhibition at 40% v/v; 28% inhibition at 40% v/v
1-Hexanol
-
68% inhibition at 40% v/v; 86% inhibition at 40% v/v
2,3,5-trichloropyridine-6-(O-methyl-O-n-butyl)-phosphate ester
-
i.e. MAT 9564
2-mercaptoethanol
-
slight inhibition at 10 mM
2-[(ethylsulfanyl)methyl]phenyl hydrogen methylcarbonimidate
-
-
3-(4-mercaptobutylthio)-1,1,1-trifluoro-2-propanone
-
-
3-n-octylthio-1,1,1-trifluoro-2-propanone
-
-
3-phenethylthio-1,1,1-trifluoropropan-2-one
3-phenylthio-1,1,1-trifluoropropan-2-one
-
-
4-nitrophenyl P-methyl-N-octylphosphonamidoate
-
-
6-mercaptohexyl acrylic ester
-
product inhibition
-
acetone
-
92% inhibition at 75% v/v
AgNO3
-
90% inhibition at 1 mM; 93% inhibition at 1 mM
alkylboronic acids
-
-
ANS
binds strongly to native cutinase as a noncompetitive inhibitor with up to 5 ANS per cutinase molecule. The first ANS molecule stabilizes cutinase. The last 4 ANS molecules decrease Tm by up to 7C
Ba2+
-
inhibits isozyme Tfu 0882 7%
BaCl2
-
14% inhibition at 1 mM; 8% inhibition at 1 mM
Benzene
-
77% inhibition at 40% v/v; 79% inhibition at 40% v/v
butanol
-
10% inhibition at 40% v/v; 17% inhibition at 40% v/v
Carbaryl
-
-
carbofuran
-
-
chlorpyrifos-methyl
-
upon chloroperoxidase oxidation, chlorpyrifos-methyl shows a very strong cutinase inhibition as compared to the corresponding oxon standard
chlorpyrifos-methyl oxon
-
-
CrCl2
-
99% inhibition at 1 mM; 99% inhibition at 1 mM
CuSO4
-
26% inhibition at 1 mM; 32% inhibition at 1 mM
D-glucose
Thcut1 mRNA is repressed by glucose
deoxycholate
-
24% inhibition at 10 mM; 25% inhibition at 10 mM
Dichloromethane
Diethyl p-nitrophenyl phosphate
diethyl-p-nitrophenyl phosphate
-
-
diethyldicarbonate
-
54% inhibition at 1 mM; 56% inhibition at 1 mM
diisopropyl fluorophosphate
dioxan
-
23% inhibition at 75% v/v
DMSO
-
49% inhibition at 75% v/v
ethanol
-
97% inhibition at 75% v/v
FeSO4
-
55% inhibition at 1 mM; 55% inhibition at 1 mM
glycerol
guanidine hydrochloride
hexyl acetate
-
63% inhibition at 40% v/v; 83% inhibition at 40% v/v
HgCl2
-
99% inhibition at 1 mM; 99% inhibition at 1 mM
Isopropanol
-
complete inhibition at 75% v/v
methanol
methomyl
-
-
MgCl2
-
15% inhibition at 1 mM; 8% inhibition at 1 mM
Mn2+
-
slight inhibition at 1 mM
n-hexane
Ni2+
-
inhibits 7% at 1 mM
O-(4-nitrophenyl) S-octyl methylphosphonothioate
-
-
O-methyl-O-(p-nitrophenyl)octylphosphonate
-
-
O-octyl-O-(p-nitrophenyl)ethylphosphonate
-
-
O-octyl-O-(p-nitrophenyl)methylphosphonate
-
-
oxidized malathion
-
oxidized malathion, contrarily to malaoxon, reveals cutinase inhibition
Paraoxon
paraoxon-methyl
-
-
PbCl2
-
53% inhibition at 1 mM; 54% inhibition at 1 mM
phenylboronic acid
-
5 mM, 63% inhibition, competitive
primicarb
-
-
propoxur
-
-
RbCl
-
93% inhibition at 1 mM; 95% inhibition at 1 mM
sodium bis(2-ethylhexyl)ester sulfosuccinic acid
-
pseudo-competitive inhibitor
Sodium deoxycholate
-
-
sodium dioctyl sulfosuccinate
-
-
Sodium dodecyl sulfate
-
competitive, detailed study of interaction with enzyme. At molar ratio of SDS:enzyme of about 10, formation of aggregates which include more than one protein molecule. At higher concentration of SDS, denaturation of protein, denatured state of enzyme is unusually compact
triethylamine
-
24% inhibition at 40% v/v
Triton X-100
-
35% inhibition at 1 mM; 55% inhibition at 1 mM
Tween-20
-
28% inhibition at 1 mM; 35% inhibition at 1 mM
Tween-80
-
24% inhibition at 1 mM; 60% inhibition at 1 mM
ZnSO4
-
59% inhibition at 1 mM; 65% inhibition at 1 mM
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
16-hydroxyhexadecanoic acid
is strongly induced in vitro by cutin monomer 16-hydroxyhexadecanoic acid
DMF
-
activates 10% at 40% v/v; activates 6% at 40% v/v
DMSO
-
activates 14% at 40% v/v; activates 9% at 40% v/v
N,N-diethyl-2-phenylacetamide
-
increases hydrolysis rates of semi-crystalline poly(ethylene terephthalate) films and fabrics for cutinase. The outer layers of the polymer will be better exposed to the enzyme in the presence of N,N-diethyl-2-phenylacetamide. Overall enhancement in colour depth of 300%
olive oil
Thcut1 mRNA is strongly induced in vitro by olive oil
-
sodium dioctyl sulfosuccinate
-
in the absence of surfactant the S54D variant catalytic activity is similar to that of the wild type cutinase, whereas L153Q and T179C variants show a lower activity
sodium taurodeoxychlate
-
activates 10% at 10 mM; activates 24% at 10 mM
-
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00067 - 63
4-nitrophenyl acetate
0.00021 - 59.2
4-nitrophenyl butyrate
7.24
4-nitrophenyl caprylate
-
pH 8.0, 25C, recombinant enzyme
0.00029 - 0.89
4-nitrophenyl hexanoate
7.14
4-nitrophenyl laurate
-
pH 8.0, 25C, recombinant enzyme
7.25
4-nitrophenyl myristate
-
pH 8.0, 25C, recombinant enzyme
0.207 - 1.7
4-nitrophenyl octanoate
2.246
4-nitrophenyl palmitate
-
pH 7.5, 30C, recombinant enzyme
0.00004 - 55.3
4-nitrophenyl valerate
0.33
p-nitrophenyl acetate
-
0.000121 - 4.33
p-nitrophenyl butyrate
0.085
p-nitrophenyl palmitate
-
0.82
p-nitrophenyl valerate
-
0.9 - 9.7
p-Nitrophenylacetate
0.27 - 1.68
p-nitrophenylbutanoate
0.36 - 3.98
p-nitrophenyldecanoate
0.45 - 3.55
p-nitrophenyldodecanoate
4.54
p-nitrophenylhexadecanoate
-
-
2.27
p-nitrophenyltetradecanoate
-
-
additional information
additional information