Any feedback?
Literature summary for 3.1.1.74 extracted from
- High-level expression and characterization of Fusarium solani cutinase in Pichia pastoris (2009), Protein Expr. Purif., 68, 104-109.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Pichia pastoris expressing the native non-tagged cutinase exhibits about 2- and 3fold higher values of protein amount and cutinase activity in the culture supernatant, respectively, than those containing the C-terminal tagged cutinase | Fusarium solani |
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | high-level secretion of cutinase in Pichia pastoris may be a promising alternative to many expression systems previously used for the large-scale production of cutinase in Saccharomyces cerevisiae as well as Escherichia coli. The functional expression of a large amount of extracellular cutinase offers the opportunity for developing an efficient high-throughput screening procedure for the improvement of enzymatic property and the development of novel biocatalysis of cutinase | Fusarium solani |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into pPICZalphaA with the Saccharomyces cerevisiae alpha-factor signal sequence and methanol-inducible alcohol oxidase promoter, additional sequences of the c-myc epitope and (His)6-tag of the vector fused to the C-terminus of cutinase, while the other expression vector is constructed without any additional sequence. Expressed in Pichia pastoris strain X-33 | Fusarium solani |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium solani | P00590 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| non-tagged and the C-terminal tagged cutinases, by diafiltration | Fusarium solani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tributyrin + H2O | - |
Fusarium solani | ? | - |
? |  |
| tricaprylin + H2O | - |
Fusarium solani | ? | - |
? | |
| triolein + H2O | - |
Fusarium solani | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cutinase | - |
Fusarium solani |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 45 | non-tagged and the C-terminal tagged cutinases | Fusarium solani |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | non-tagged and the C-terminal tagged cutinases | Fusarium solani |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
