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Literature summary for 3.1.1.74 extracted from
- A novel protein elicitor (SsCut) from Sclerotinia sclerotiorum induces multiple defense responses in plants (2014), Plant Mol. Biol., 86, 495-511.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene SsCut, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21, expression in Nicotiana benthamiana leaves, quantitative real-time polymerase chain reaction analysis | Sclerotinia sclerotiorum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S117A | site-directed mutagenesis, the mutation causes a 99% reduction in enzyme activity and also completely abolishes the elicitor activity of the protein | Sclerotinia sclerotiorum |
| Y116A | site-directed mutagenesis, the mutation causes a 97% reduction in enzyme activity and also abolishes the elicitor activity of the protein | Sclerotinia sclerotiorum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| PMSF | - |
Sclerotinia sclerotiorum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20400 | - |
x * 20400, sequence calculation, x * 45000, recombinant His-tagged enzyme, SDS-PAGE | Sclerotinia sclerotiorum |
| 45000 | - |
x * 20400, sequence calculation, x * 45000, recombinant His-tagged enzyme, SDS-PAGE | Sclerotinia sclerotiorum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sclerotinia sclerotiorum | A7EQQ8 | gene SsCut | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity and anion exchange chromatography and gel filtration | Sclerotinia sclerotiorum |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 20400, sequence calculation, x * 45000, recombinant His-tagged enzyme, SDS-PAGE | Sclerotinia sclerotiorum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Sclerotinia sclerotiorum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Sclerotinia sclerotiorum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Sclerotinia sclerotiorum | sequence calculation | - |
5.47 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | residues Ser117, Asp169, and His182 form the active site | Sclerotinia sclerotiorum |
| physiological function | the enzyme is an elicitor that triggers defense responses in plants, recombinant His-tagged enzyme causes cell death in Arabidopsis thaliana, Glycine max, Brassica napus, Oryza sativa, Zea mays, and Triticum aestivum, indicating that both dicot and monocot species are responsive to the elicitor. The elicitation of Nicotiana tabacum is effective in the induction of the activities of hydrogen peroxide, phenylalanine ammonia-lyase, peroxides, and polyphenol oxidase. Phenotypes, detailed overview | Sclerotinia sclerotiorum |
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Release 2023.1 (January 2023)
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