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Literature summary for 3.1.1.74 extracted from

  • Pedersen, S.; Nesgaard, L.; Baptista, R.P.; Melo, E.P.; Kristensen, S.R.; Otzen, D.E.
    pH-dependent aggregation of cutinase is efficiently suppressed by 1,8-ANS (2006), Biopolymers, 83, 619-629.
    View publication on PubMed
Search for more literature for 3.1.1.74

General Stability

General Stability Organism
ANS binds strongly to native cutinase as a noncompetitive inhibitor with up to 5 ANS per cutinase molecule. The first ANS molecule stabilizes cutinase. The last 4 ANS molecules decrease Tm by up to 7°C Fusarium solani

Inhibitors

Inhibitors Comment Organism Structure
ANS binds strongly to native cutinase as a noncompetitive inhibitor with up to 5 ANS per cutinase molecule. The first ANS molecule stabilizes cutinase. The last 4 ANS molecules decrease Tm by up to 7°C Fusarium solani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000121
-
 p-nitrophenyl butyrate 20°C, pH 8, no additive Fusarium solani

Organism

Organism UniProt Comment Textmining
Fusarium solani P00590 recombinantly expressed in Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl butyrate + H2O
-
 Fusarium solani p-nitrophenol + butyrate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 ANS binds strongly to native cutinase as a noncompetitive inhibitor with up to 5 ANS per cutinase molecule. The first ANS molecule stabilizes cutinase. The last 4 ANS molecules decrease Tm-value by up to 7°C Fusarium solani
40
-
 0.08 mM cutinase, pH 8.0, no additive, Tm-value is 40.3°C Fusarium solani
48
-
 0.08 mM cutinase or 0.005 mM cutinase, pH 8.0, 2 M urea, Tm-value 47.5°C Fusarium solani
52
-
 0.06 mM cutinase, pH 8.0, Tm-value is 52.0°C Fusarium solani
55
-
 0.08 mM cutinase, pH 8.0, 20 mM 0.5 M L-Arg, Tm-value Fusarium solani
56
-
 0.005 mM cutinase, pH 8.0, 20 mM 0.5 M L-Arg, Tm-value is 56.4°C Fusarium solani
57
-
 0.04 mM cutinase, pH 8.0, Tm-value is 57.3°C Fusarium solani
59
-
 0.0001 mM cutinase, pH 8.0, Tm-value is 59.3°C Fusarium solani
59
-
 0.005 mM cutinase, pH 8.0, Tm-value is 59.3°C Fusarium solani

pI Value

Organism Comment pI Value Maximum pI Value
Fusarium solani
-
-
 8