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Literature summary for 3.1.1.74 extracted from
- Identification and comparison of cutinases for synthetic polyester degradation (2012), Appl. Microbiol. Biotechnol., 93, 229-240.
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | biotechnological applications of cutinases for synthetic polyester degradation | Humicola insolens |
| degradation | biotechnological applications of cutinases for synthetic polyester degradation | Aspergillus oryzae |
| degradation | biotechnological applications of cutinases for synthetic polyester degradation | Fusarium solani |
| degradation | biotechnological applications of cutinases for synthetic polyester degradation | Aspergillus fumigatus |
| degradation | biotechnological applications of cutinases for synthetic polyester degradation | Alternaria brassicicola |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | comparison of kinetics of cutinases from different organisms, overview | Humicola insolens | |
| additional information | - |
additional information | comparison of kinetics of cutinases from different organisms, overview | Aspergillus oryzae | |
| additional information | - |
additional information | comparison of kinetics of cutinases from different organisms, overview | Fusarium solani | |
| additional information | - |
additional information | comparison of kinetics of cutinases from different organisms, overview | Aspergillus fumigatus | |
| additional information | - |
additional information | comparison of kinetics of cutinases from different organisms, overview | Alternaria brassicicola | |
| 0.00136 | - |
4-nitrophenyl butyrate | pH 7.5, 25°C | Fusarium solani |  |
| 0.00196 | - |
4-nitrophenyl butyrate | pH 7.5, 25°C | Alternaria brassicicola | |
| 0.0025 | - |
4-nitrophenyl butyrate | pH 7.5, 25°C | Humicola insolens | |
| 0.00896 | - |
4-nitrophenyl butyrate | pH 7.5, 25°C | Aspergillus fumigatus | |
| 0.029 | - |
4-nitrophenyl butyrate | pH 7.5, 25°C | Aspergillus oryzae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alternaria brassicicola | - |
- |
- |
| Aspergillus fumigatus | - |
- |
- |
| Aspergillus oryzae | - |
- |
- |
| Fusarium solani | - |
- |
- |
| Humicola insolens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl butyrate + H2O | - |
Humicola insolens | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Aspergillus oryzae | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Fusarium solani | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Aspergillus fumigatus | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Alternaria brassicicola | 4-nitrophenol + butyrate | - |
? | |
| additional information | the cutinase demonstrates enhanced poly(epsilon-caprolactone) hydrolysis at high temperatures and under all pH value. The cutinase shows activity on 4-nitrophenyl butyrate | Humicola insolens | ? | - |
? | |
| additional information | the cutinase demonstrates enhanced poly(epsilon-caprolactone) hydrolysis at high temperatures and under all pH value. The cutinase shows activity on 4-nitrophenyl butyrate | Aspergillus oryzae | ? | - |
? | |
| additional information | the cutinase demonstrates enhanced poly(epsilon-caprolactone) hydrolysis at high temperatures and under all pH value. The cutinase shows activity on 4-nitrophenyl butyrate | Aspergillus fumigatus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Humicola insolens |
| 25 | - |
assay at | Aspergillus oryzae |
| 25 | - |
assay at | Fusarium solani |
| 25 | - |
assay at | Aspergillus fumigatus |
| 25 | - |
assay at | Alternaria brassicicola |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme is unstable and functions poorly at high temperatures as well as at acidic pH conditions, differential scanning calorimetry thermograms of cutinase, overview | Humicola insolens |
| additional information | - |
the enzyme is unstable and functions poorly at high temperatures as well as at acidic pH conditions, differential scanning calorimetry thermograms of cutinase, overview | Aspergillus oryzae |
| additional information | - |
the enzyme is unstable and functions poorly at high temperatures as well as at acidic pH conditions, differential scanning calorimetry thermograms of cutinase, overview | Fusarium solani |
| additional information | - |
the enzyme is unstable and functions poorly at high temperatures as well as at acidic pH conditions, differential scanning calorimetry thermograms of cutinase, overview | Aspergillus fumigatus |
| additional information | - |
the enzyme is unstable and functions poorly at high temperatures as well as at acidic pH conditions, differential scanning calorimetry thermograms of cutinase, overview | Alternaria brassicicola |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0072 | - |
4-nitrophenyl butyrate | pH 7.5, 25°C | Fusarium solani | |
| 0.0163 | - |
4-nitrophenyl butyrate | pH 7.5, 25°C | Aspergillus oryzae | |
| 0.022 | - |
4-nitrophenyl butyrate | pH 7.5, 25°C | Alternaria brassicicola | |
| 0.072 | - |
4-nitrophenyl butyrate | pH 7.5, 25°C | Humicola insolens | |
| 0.0845 | - |
4-nitrophenyl butyrate | pH 7.5, 25°C | Aspergillus fumigatus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | assay at | Humicola insolens |
| 7.5 | 8 | assay at | Aspergillus oryzae |
| 7.5 | 8 | assay at | Fusarium solani |
| 7.5 | 8 | assay at | Aspergillus fumigatus |
| 7.5 | 8 | assay at | Alternaria brassicicola |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | - |
Humicola insolens |
| 3 | 8 | - |
Aspergillus oryzae |
| 3 | 8 | - |
Fusarium solani |
| 3 | 8 | - |
Aspergillus fumigatus |
| 3 | 8 | - |
Alternaria brassicicola |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme is unstable and functions poorly at high temperatures as well as at acidic pH conditions, biophysical parameters of cutinase as a function of pH, overview | Humicola insolens |
| additional information | - |
the enzyme is unstable and functions poorly at high temperatures as well as at acidic pH conditions, biophysical parameters of cutinase as a function of pH, overview | Aspergillus oryzae |
| additional information | - |
the enzyme is unstable and functions poorly at high temperatures as well as at acidic pH conditions, biophysical parameters of cutinase as a function of pH, overview | Fusarium solani |
| additional information | - |
the enzyme is unstable and functions poorly at high temperatures as well as at acidic pH conditions, biophysical parameters of cutinase as a function of pH, overview | Aspergillus fumigatus |
| additional information | - |
the enzyme is unstable and functions poorly at high temperatures as well as at acidic pH conditions, biophysical parameters of cutinase as a function of pH, overview | Alternaria brassicicola |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | biophysical parameters of cutinase as a function of pH, overview | Humicola insolens |
| additional information | biophysical parameters of cutinase as a function of pH, overview | Aspergillus oryzae |
| additional information | biophysical parameters of cutinase as a function of pH, overview | Fusarium solani |
| additional information | biophysical parameters of cutinase as a function of pH, overview | Aspergillus fumigatus |
| additional information | biophysical parameters of cutinase as a function of pH, overview | Alternaria brassicicola |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0053 | - |
4-nitrophenyl butyrate | pH 7.5, 25°C | Fusarium solani | |
| 0.0093 | - |
4-nitrophenyl butyrate | pH 7.5, 25°C | Aspergillus fumigatus | |
| 0.0162 | - |
4-nitrophenyl butyrate | pH 7.5, 25°C | Alternaria brassicicola | |
| 0.029 | - |
4-nitrophenyl butyrate | pH 7.5, 25°C | Humicola insolens | |
| 0.0567 | - |
4-nitrophenyl butyrate | pH 7.5, 25°C | Aspergillus oryzae | |
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