Cloned (Comment) | Organism |
---|---|
- |
Monilinia fructicola |
Protein Variants | Comment | Organism |
---|---|---|
H173L | 36% of wild-type activity | Monilinia fructicola |
S103A | 231% of wild-type activity | Monilinia fructicola |
S103T | 38% of wild-type activity | Monilinia fructicola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.23 | - |
p-nitrophenylbutanoate | 36°C, wild-type | Monilinia fructicola | |
1.45 | - |
p-nitrophenylbutanoate | 36°C, mutant H173L | Monilinia fructicola | |
1.5 | - |
p-nitrophenylbutanoate | 36°C, mutant S103T | Monilinia fructicola | |
1.68 | - |
p-nitrophenylbutanoate | 36°C, mutant S103A | Monilinia fructicola |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18134 | - |
x * 20227, precursor protein, x * 18134, mature form, calculated | Monilinia fructicola |
20227 | - |
x * 20227, precursor protein, x * 18134, mature form, calculated | Monilinia fructicola |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Monilinia fructicola | Q8TGB8 | enzyme precursor | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | sequence contains a 20 amino-acid secretory signal, one potential N-linked glycosilation site, a protein kinase C phosphorylation site, a lipase active serine and a cutinase active serine | Monilinia fructicola |
Purification (Comment) | Organism |
---|---|
His-tagged protein, expressed in Pichia pastoris | Monilinia fructicola |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenylbutanoate + H2O | - |
Monilinia fructicola | p-nitrophenol + butanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 20227, precursor protein, x * 18134, mature form, calculated | Monilinia fructicola |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
p-nitrophenylbutanoate | 36°C, mutant H173L | Monilinia fructicola | |
0.25 | - |
p-nitrophenylbutanoate | 36°C, mutant S103T | Monilinia fructicola | |
0.66 | - |
p-nitrophenylbutanoate | 36°C, wild-type | Monilinia fructicola | |
1.5 | - |
p-nitrophenylbutanoate | 36°C, mutant S103A | Monilinia fructicola |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Monilinia fructicola | mature form, calculated | - |
8.2 |
Monilinia fructicola | precursor protein, calculated | - |
8.4 |