Cloned (Comment) | Organism |
---|---|
transgenic Arabidopsis plants expressing GFP-CDEF1 fusion protein driven by the 35S promoter. CDEF1 expressed with a 6 x His tag at its C-terminus in suspension cultures of BY-2 tobacco cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | CDEF1-deficient mutant (SALK-014093) that carries a T-DNA insertion in the coding region of CDEF1, shows no abnormal phenotypes, such as reduced fertility or reduced lateral root emergence | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39200 | - |
sequence analysis | Arabidopsis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis | - |
- |
- |
Arabidopsis thaliana | Q9SZW7 | - |
- |
Purification (Comment) | Organism |
---|---|
on Ni2+ affinity column | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | CDEF1 is secreted to the extracellular space in leaves | Arabidopsis thaliana | - |
pollen | - |
Arabidopsis | - |
pollen | in mature pollen grains within non-dehiscent anthers | Arabidopsis thaliana | - |
pollen tube | pollen tubes that are germinating on the stigma | Arabidopsis thaliana | - |
root | at the zone of lateral root emergence | Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
45 | - |
at 30°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | - |
Arabidopsis thaliana | 4-nitrophenol + butyrate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39200, calculated from sequence | Arabidopsis |
Synonyms | Comment | Organism |
---|---|---|
CDEF1 | - |
Arabidopsis |
CDEF1 | - |
Arabidopsis thaliana |
cuticle destructing factor 1 | - |
Arabidopsis |
cuticle destructing factor 1 | - |
Arabidopsis thaliana |
cutinase | - |
Arabidopsis |
cutinase | - |
Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
activity is reduced to less than 10% of the maximum activity by heating for 10 min at 99°C | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | CDEF1 gene is not expressed in leaves of wild-type plants. Is expressed at low levels in roots | down |
Arabidopsis thaliana | CDEF1 gene is expressed in leaves of transgenic 35S::CDEF1 plants. CDEF1 gene is highly expressed in mature pollen | up |
General Information | Comment | Organism |
---|---|---|
physiological function | CDEF1 is a plant cutinase, recombinant CDEF1 protein has esterase activity. Ectopic expression of CDEF1 driven by the 35S promoter causes fusion of organs, including leaves, stems and flowers, and increased surface permeability. CDEF1 is involved in the penetration of the stigma by pollen tubes. CDEF1 degrades cell wall components to facilitate the emergence of the lateral roots | Arabidopsis thaliana |
physiological function | ectopic expression of CDEF1 driven by the 35S promoter causes fusion of organs, including leaves, stems and flowers, and increased surface permeability | Arabidopsis |